Evaluasi Sitotoksik Alfa Mangostin Pada Kultur Sel Leukosit Manusia Secara In Vitro dan Uji Aktivitas Antioksidan

Wahyuni, Fatma Sri; Sudji, Ikhwan Resmala; Amaliyah, Rizki Amaliyah

doi:10.25077/jsfk.5.3.201-206.2018

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…The isolated cells were counted using hemocytometer by taking 10 µL of leukocyte cell suspension, put in a microtube, added with 10 µL of trypan blue, and then pipetting to make it homogeneous. After that, take 10 µL of the mixture, placed in counting chamber, then counted under an inverted microscope [11].…”

Section: Cell Linesmentioning

confidence: 99%

In Vitro Effect of Tetraprenyltoluquinone on Normal Human Leukocyte Cell

Hefni¹,

Wahyuni²,

Dharma³

et al. 2021

Advances in Health Sciences Research

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Tetraprenyltoluquinone is a compound that was successfully isolated from the hexane fraction of stem bark Garcinia cowa, Roxb. In previous studies, tetraprenyltoluquinone is known to have anticancer and antiinflammatory activity. The aim of the study is to investigate the impact of tetraprenyltoluquinone on normal human leukocyte cell cultures. Tetraprenyltoluquinone with a concentration between 3.125-1000 μg/mL was tested for cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and measured at λ 570 nm using a microplate reader. The results showed that tetraprenyltoluquinone was not toxic to human leukocyte cell cultures and had an IC50 of 2588.938 μg/mL.

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Section: Cell Linesmentioning

confidence: 99%

In Vitro Effect of Tetraprenyltoluquinone on Normal Human Leukocyte Cell

Hefni¹,

Wahyuni²,

Dharma³

et al. 2021

Advances in Health Sciences Research

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“…α-mangostin is a yellow amorphous crystal with a melting point of 180-182°C, giving it absorption in UV light at αmax of 215, 243, and 317 nm. The α-mangostin compound has anticancer, antituberculosis, antihistamine, and antioxidant activity (Sri Wahyuni et al, 2018;Maligan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

α-Mangostin Content of Mangosteen Leaves (Garcinia mangostana L.) Based on Different Growing Conditions

Rahmiyani,

Yuliana,

Rukmana

et al. 2023

Planta Tropika

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Environmental factors, such as soil height, plant age, soil conditions, maintenance, and plant seeds influence the content of secondary metabolites in an organism. The growing conditions can also affect the content of active compounds in plants. Xanthone is an active compound in Garcinia mangostana Linn. One of its derivatives is α-mangostin, which has antioxidant, anticancer, antituberculosis and antihistamine effects. This study aimed to determine the effects of growing conditions on the levels of α-mangosteen in mangosteen leaf extract using Thin Layer Chromatography-Densitometry. Standard solution of α-mangosteen and ethyl acetate extract of mangosteen leaves (A and B samples) were analyzed with Camag TLC Scanner 3 using silica gel 60 F254 stationary phase and chloroform-ethyl acetate mobile phase (9:1). The results showed that the Rf values for standard solution of α-mangostin, sample A, and sample B were 0.65, 0.62, and 0.62, respectively. Meanwhile, the levels obtained from samples A and B were 2.10% ± 0.0755 and 2.07% ± 0.0321, respectively. Different growing conditions did not affect the level of α-mangostin ethyl acetate extract of mangosteen leaves.

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The Effectiveness of Herbal Mouthwash with Mangosteen Peel Extract in Inhibiting Dental Plaque Formation

Hendiani,

Fitriani,

Prasetyo

et al. 2024

European Journal of General Dentistry

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Objective Dental plaque control is important for preventing periodontal tissue diseases. Dental plaque control therapy is enhanced when supported by adjunctive therapy, including the use of mangosteen peel extract mouthwash. Mangosteen peel extract contains α-mangostin, saponins, alkaloids, tannins, flavonoids, quinones, and triterpenoids, which have antibacterial properties against bacteria that cause dental plaque. This study aims to determine the effectiveness of mangosteen peel extract mouthwash at concentrations of 2, 4, and 6% in inhibiting plaque formation. Materials and Methods The study used a quasi-experimental design with pre- and posttreatment examinations. Samples were taken using purposive sampling on 32 patients of Periodontology Clinic of Padjadjaran University Dental Hospital. The patients underwent prophylactic treatment (scaling), then the dental plaque index was measured using the Q-ray Cam Pro and the Loe and Silness Index before (day 1) and after (day 3) gargling with distilled water or mangosteen peel extract mouthwash at concentrations of 2, 4, and 6% for 2 days without oral hygiene in the maxillary area. The data were analyzed using the Wilcoxon test, analysis of variance (ANOVA), and the Kruskal–Wallis test. Results A phytochemical analysis revealed that the mangosteen peel extract contains antibacterial compounds such as flavonoids, saponins, polyphenols, quinones, and triterpenoids. The mangosteen peel extract mouthwash group exhibited lower mean differences in plaque index compared with the aquades group. The 2% mangosteen peel extract mouthwash shows the smallest mean difference of 0.25 in the Q-ray Cam Pro examination and 0.062 in the Loe and Silness Index examination. Conclusion Mouthwash with 2, 4, and 6% mangosteen peel extract has an effect in inhibiting dental plaque formation, with 2% concentration exhibiting the best inhibitory effect on dental plaque formation.

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Evaluasi Sitotoksik Alfa Mangostin Pada Kultur Sel Leukosit Manusia Secara In Vitro dan Uji Aktivitas Antioksidan

Cited by 3 publications

References 4 publications

In Vitro Effect of Tetraprenyltoluquinone on Normal Human Leukocyte Cell

In Vitro Effect of Tetraprenyltoluquinone on Normal Human Leukocyte Cell

α-Mangostin Content of Mangosteen Leaves (Garcinia mangostana L.) Based on Different Growing Conditions

The Effectiveness of Herbal Mouthwash with Mangosteen Peel Extract in Inhibiting Dental Plaque Formation

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