Evaluate the Shelf Life of Rhizobium Carrier Based Biofertilizer Stored at Different Temperatures at Different Intervals

Thirumal, G.; Reddy, R. Subhash; Triveni, S.; Damodarachari, K.; Bhavya, K.

doi:10.20546/ijcmas.2017.607.094

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…However, the selection of suitable supporting materials for bacterial inoculant preparation remains challenging [34]. For this reason, assessment of inoculated bacteria in supporting materials is one strategy for the enhancement of crop productivity [35]. In this study, PGP Ensifer adhaerens MSN12 and Bacillus cereus MEN8 exhibited their inherent potential to synthesize IAA.…”

Section: Discussionmentioning

confidence: 91%

Bioformulation Containing Cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8 for the Nutrient Enhancement of Cicer arietinum L.

Baliyan

Qureshi

Jaremko

et al. 2022

Plants

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Here we examine the effects of different carrier based bioinoculants on the growth, yield and nutritional value of chickpea and on associated soil nutrients. A consortium of two taxonomically distinct endophytic bacteria—Ensifer adhaerens MSN12 and Bacillus cereus MEN8—have promising plant growth promoting (PGP) attributes. We demonstrate their delivery from the laboratory to the field via the formulation of an effective bioinoculant with economic and accessible carriers. Sugarcane straw ash (SCSA) was found to be an efficient carrier and bioformulation for enhancing viability and shelf-life of strains up to 12 months. A bioformulation containing an SCSA-based consortium (MSN12 + MEN8) increased seed germination by 7%, plant weight by 29%, length by 17%, seed-yield by 12%, harvesting index by 14% and proximate nutritional constituents by 20% over consortium treatment without SCSA. In addition, the bioformulation of post-harvest treated soil improved the physico-chemical properties of the soil in comparison to a pre-sowing SCSA-based bioformulation treated crop, being fortified in different proximate nutritional constituents including dry matter (30%), crude protein (45%), crude fiber (35%), and ether extract (40%) in comparison to the control. Principal component analysis and scattered matrix plots showed a positive correlation among the treatments, which also validates improvement in the soil nutrient components and proximate constituents by T6 treatment (MSN12 + MEN8 + SCSA). The above results suggest efficiency of SCSA not only as a carrier material but also to support microbial growth for adequate delivery of lab strains as a substitute for chemi-fertilizers.

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Section: Discussionmentioning

confidence: 91%

Bioformulation Containing Cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8 for the Nutrient Enhancement of Cicer arietinum L.

Baliyan

Qureshi

Jaremko

et al. 2022

Plants

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“…Pada penyimpanan carrier based bio-innoculant pada suhu 4°C dan 28°C selama 8 bulan diperoleh bahwa populasi rhizobium menurun. Tingkat kelangsungan hidup lebih tinggi pada penyimpanan suhu 4°C dibandingkan dengan 28±2°C karena kadar air yang rendah pada carrier innocullant yang disimpan pada temperature 28±2°C (Thirumal et al, 2017).…”

Section: Metode Penelitianunclassified

Pembuatan Pupuk Padat Dari Hasil Samping Biogas Di Gunungkidul

Praharasti¹,

Febrisiantosa²,

Jatmiko³

et al. 2023

JPPT

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Pemanfaatan hasil samping biogas diharapkan dapat meningkatkan aspek manfaat dari keberadaan reaktor biogas. Selain aspek ketersediaan energi, keberadaan teknologi biogas menjadi solusi yang sesuai untuk memanfaatkan kotoran ternak menjadi sumber input pada pertanian skala kecil. Selain menghasilkan gas metan, reaktor biogas juga menghasilkan hasil samping berupa padatan dan cairan dari pipa keluaran sistem reaktor biogas. Pada kegiatan ini, dilakukan 2 fokus kegiatan yakni formulasi pupuk padat dari limbah padat biogas dan pelatihan formulasi pupuk limbah padat biogas kepada Kelompok Tani dan Ternak Sedyo Mulyo. Untuk formulasinya, limbah padat dan cair dari reaktor biogas tersebut dipisahkan untuk diproses lebih lanjut(slurry/sludge) menjadi pupuk atau nutrisi untuk tanaman. Pupuk padat dengan menggunakan limbah padat biogas dicampur dengan biomassa lain. Hasil pengujian formula pupuk menunjukkan nilai pH yakni sekitar 6-7. Untuk nilai kadar airnya yakni sebesar 52-67%. Perkembangan lebih lanjut diperlukan upaya untuk penyesuaian kadar air pada formulasi yang dikembangkan. Kadar air diperkirakan mempengaruhi umur simpan, kaitannya dengan jumlah mikroba hidup sebagai kontrol kualitas.

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“…Thirumal et al [22] reported that the high viable cell of Rhizobium biofertilizer was observed in SA formulation at room temperature after eight months of storage. Similarly, Rhodopseudomonas palustris was also found to survive well and maintain at around 10 log CFU/mL in a growth medium amended with 0.5% SA [23].…”

Section: Introductionmentioning

confidence: 99%

Sustainable Agronomic Valorization of Unsulfured Molasses and Defatted Soybean Meal as an Optimized Formulation of Bio-Organic Fertilizer Enriched with High Cell Density P-Solubilizing Bacteria

et al. 2021

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The application of plant beneficial bioinoculants such as phosphate solubilizing bacteria is a sustainable approach to expanding crop performance in agriculture. However, bioinoculant strains, particularly non-sporulating bacteria are often exposed to detrimental conditions throughout the production process and a long period of storage. This will negatively influence their viable cell density and eventually limit its efficacy in the field. To overcome such a scenario, an optimal formulation of biofertilizer should be prioritized. In this report, a sustainable valorization of molasses and defatted soybean meal as formulation of biofertilizer enriched with Enterobacter hormaechei 40a was proposed. Through the two-level factorial design and central composite design, the optimal formulation and fermentation conditions of bio-organic fertilizer to achieve maximum cell density of strain 40a were achieved. The highest cell density of strain 40a in the optimized molasses-DSM (OMD) medium was 12.56 log CFU/mL after 24 h which was 99.7% accuracy towards the predicted value. Interestingly, the solubilized P was increased by 62.4% in the OMD medium (174.07 µg/mL P) as compared to the standard P medium (65.38 µg/mL P). The shelf life of strain 40a after 180 days of storage was improved significantly around 10 log CFU/mL, when the OMD medium was amended with 0.1% sodium alginate. The strategy described here offers opportunities for agronomic formulation and large-scale bio-organic fertilizer production in the agriculture industry.

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Evaluate the Shelf Life of Rhizobium Carrier Based Biofertilizer Stored at Different Temperatures at Different Intervals

Cited by 8 publications

References 12 publications

Bioformulation Containing Cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8 for the Nutrient Enhancement of Cicer arietinum L.

Bioformulation Containing Cohorts of Ensifer adhaerens MSN12 and Bacillus cereus MEN8 for the Nutrient Enhancement of Cicer arietinum L.

Pembuatan Pupuk Padat Dari Hasil Samping Biogas Di Gunungkidul

Sustainable Agronomic Valorization of Unsulfured Molasses and Defatted Soybean Meal as an Optimized Formulation of Bio-Organic Fertilizer Enriched with High Cell Density P-Solubilizing Bacteria

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