Evaluating antimicrobial activities of Acanthus ilicifolius L. and Heliotropium curassavicum L against bacterial pathogens: an in-vitro study

Pothiraj, C.; Paulraj, Balaji; Shanthi, R.; Gobinath, M.; Babu, R. Suresh; Munirah, Abdullah Al-Dosary; Hatamleh, Ashraf Atef; Kumar, Kamatchi Ramesh; Veeramanikandan, V.; Arumugam, R.

doi:10.1016/j.jiph.2021.10.013

Cited by 23 publications

(12 citation statements)

References 27 publications

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“…Further, this extract showed antimicrobial activity against Klebsiella pneumoniae and Staphylococcus aureus. Similar observations have been reported [19] though they also showed a significant effect against E coli which was not observed in our study. Methanolic extracts have also shown effects against Salmonella typhi and Candida albicans [16].…”

Section: Discussionsupporting

confidence: 92%

Evaluation of Antioxidant, Antimicrobial, Anticancer, and Wound Healing Properties of Leaf Extracts of Acanthus Ilicifolius L.

JOHNEY

RAGUNATHAN

2023

Int J Curr Pharm Sci

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Objective: To investigate the in vitro antioxidant, antimicrobial, anticancer, and wound healing properties of leaf extracts of Acanthus ilicifolius. Methods: Acanthus ilicifolius leaves were dried and extracts were prepared using different solvents using cold extraction method. The preliminary phytochemical analysis and quantitative methods were carried out using standard procedures. In vitro antioxidant activity was evaluated using DPPH assay. Disc diffusion method was employed to determine the antimicrobial activities of the extracts. The methanolic extract was subjected to FTIR and GC-MS analysis for characterization of phytochemicals. This extract was further subjected to HPLC analysis for characterization and quantification. The antiproliferative effects of methanolic extract was determined using MTT assay and wound healing properties were determined using Cell migration assay. Results: The highest levels of phenol and flavonoids were detected in aqueous extracts, followed by chloroform. Methanolic extract showed the highest antioxidant activity as well as significant antibacterial activity against Klebsiella pneumoniae and Staphylococcus aureus. Squalene, a metabolite with known pharmaceutical applications, was identified in GC-MS, which was further confirmed and quantified using HPLC analysis. The methanolic extract showed cytotoxic activity against the cervical cancer cell line and showed a significant beneficial effect on wound healing in the L929 cell line. Conclusion: The results obtained in this study revealed that the leaf extracts of Acanthus ilicifolius possess potent antioxidant, antimicrobial, cytotoxic and wound-healing properties. These activities are due to bioactive components present in the extracts whose identification could lead to the development of new therapeutic agents.

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Section: Discussionsupporting

confidence: 92%

Evaluation of Antioxidant, Antimicrobial, Anticancer, and Wound Healing Properties of Leaf Extracts of Acanthus Ilicifolius L.

JOHNEY

RAGUNATHAN

2023

Int J Curr Pharm Sci

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“…The detected amounts of HBOA in the roots, stems, and leaves were 1,226, 557, and 205 µg/g, respectively. A. ilicifolius Linnaeus is one of the medicinal mangrove plants used as a traditional drug in China and other countries in the Asia-Pacific region (1,36). Meanwhile, 2-benzoxazolone alkaloids are important active components (7).…”

Section: Validation Of the Mispe Protocol In Real Samplesmentioning

confidence: 99%

Molecularly imprinted polymer-specific solid-phase extraction for the determination of 4-hydroxy-2(3H)benzoxazolone isolated from Acanthus ilicifolius Linnaeus using high-performance liquid chromatography-tandem mass spectrometry

Lin

Yong

et al. 2022

Front. Nutr.

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The minor constituent found in Acanthus ilicifolius Linnaeus, 4-hydroxy-2 (3H) benzoxazolone alkaloid (HBOA), has a range of versatile applications. Herein, a quick and straightforward method for extracting HBOA from A. ilicifolius Linnaeus was proposed. HBOA was used as a template, whereas methacrylic acid, ethylene glycol dimethacrylate, and acetonitrile were used as functional monomers, cross-linkers, and porogens, respectively. Molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization, and their adsorption isotherms, dynamics, and selective binding ability were characterized and analyzed. The results showed that the adsorption amount of the template was 90.18 mg/g. The MIPs were used as solid-phase extraction fillers and actual sample extraction columns, with a linear range of 0–100 μg/L, average recovery of 78.50–101.12%, and a relative standard deviation of 1.20–3.26%. The HBOA concentrations in the roots, stems, and leaves were 1,226, 557, and 205 μg/g, respectively. In addition, MIP–SPE was successfully used in isolating and purifying HBOA from different parts of A. ilicifolius Linnaeus, indicating its effectiveness in extracting and determining HBOA in other herbs.

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“…The presence of phytochemicals in the leaves may be the cause of the extracts' capacity to lower the diabetic rats' blood glucose levels. According to the findings, the extract's potency was most pronounced at doses of 100 mg/kg and 200 mg/kg (Pothiraj et al, 2021). This could be seen in the results obtained in all the assay carried out and across all the compartments of the treated animals.…”

Section: Resultsmentioning

confidence: 68%

“…Compared to control and glibenclamidetreated rats, the GSH and SOD values in the rats' liver, kidney, testicles, and plasma were considerably higher in the extract-treated animals (Tables 2 and 3). Pothiraj et al (2021) observed a high concentration of phenols, alkaloids, tannins, saponins, flavonoids and carotenoids in the young leaves of C. acuminata. The presence of phytochemicals in the leaves may be the cause of the extracts' capacity to lower the diabetic rats' blood glucose levels.…”

Section: Resultsmentioning

confidence: 85%

Antidiabetic and Antioxidative Potentials of Aqueous Extract of Cola Acuminata Leaves in Alloxan-Induced Diabetes Mellitus in Male Albino Rats

Victoria¹,

Stephen²,

Ruth³

et al. 2023

AAVS

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Numerous body organs (eyes, liver, kidney and testes) eventually suffer damage as a result of elevated blood glucose levels, which are a defining feature of the metabolic disorder known as diabetes mellitus. Researchers are in search for safe drugs that can bring a total recovery from diabetes. Thirty (35) male wistar rats were divided into six (7) groups of five animals each to study the antidiabetic and antioxidative properties of young Cola acuminata leaves aqueous extract. Diabetes was induced using alloxan monohydrate.5 ml of normal saline was given to group I , group 2 received 5 mg/kg/ body weight of the standard drug glibenclamide, group 3 was given 140 mg/kg of alloxan while groups Young C. acuminata leaves aqueous extract was administered orally in graded doses to the diabetic animals (50mg/kg, 100mg/kg, 200mg/kg and 400mg/kg bodyweight) and glibenclamide. Results showed a significant reduction of blood glucose level in the extract-treated rats (p <0.5) especially at 200 mg/kg and 400 mg/kg body weight. Samples were obtained for some biochemical (parameters) related to oxidant and antioxidants such as antioxidant enzymes, Reduced glutathione (GSH) activity, Superoxide dismutase (SOD) activity and Malondialdehyde (MDA) activity. When compared control and glibenclamide-treated rats, the antioxidant activity of the extract increases with advancing the dose of treatment. According to the findings, Cola acuminata young leaves have comparable antidiabetic efficacy to that of a glibenclamide administration. This suggests a promising prospect in developing novel drugs for treating diabetes mellitus.

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Evaluating antimicrobial activities of Acanthus ilicifolius L. and Heliotropium curassavicum L against bacterial pathogens: an in-vitro study

Cited by 23 publications

References 27 publications

Evaluation of Antioxidant, Antimicrobial, Anticancer, and Wound Healing Properties of Leaf Extracts of Acanthus Ilicifolius L.

Evaluation of Antioxidant, Antimicrobial, Anticancer, and Wound Healing Properties of Leaf Extracts of Acanthus Ilicifolius L.

Molecularly imprinted polymer-specific solid-phase extraction for the determination of 4-hydroxy-2(3H)benzoxazolone isolated from Acanthus ilicifolius Linnaeus using high-performance liquid chromatography-tandem mass spectrometry

Antidiabetic and Antioxidative Potentials of Aqueous Extract of Cola Acuminata Leaves in Alloxan-Induced Diabetes Mellitus in Male Albino Rats

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