Evaluating different approaches for the quantification of oomycete apple replant pathogens, and their relationship with seedling growth reductions

Moein, S.; Mazzola, Mark; Spies, Chris; McLeod, A.

doi:10.1007/s10658-018-01652-4

Cited by 15 publications

(5 citation statements)

References 29 publications

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“…In a previous study, we sequenced field soil supporting different adult densities in the study site and found that greater pathogen frequency was significantly associated with reduced seedling performance for almost all focal species ( 24 ). Similar results were also reported in recent studies [e.g., ( 25 )], which confirmed that relative and absolute pathogen DNA quantities in seedling roots were significantly correlated with the proportion of roots infected and that infection reduced seedling growth. Hence, the accumulation of plant pathogens signified by higher relative abundance of pathogenic OTU reads led to more negative effects on seedling performance at low SR and PD (Figs.…”

Section: Discussionsupporting

confidence: 92%

Soil microbes drive phylogenetic diversity-productivity relationships in a subtropical forest

et al. 2019

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The relationship between plant diversity and productivity and the mechanisms underpinning that relationship remain poorly resolved in species-rich forests. We combined extensive field observations and experimental manipulations in a subtropical forest to test how species richness (SR) and phylogenetic diversity (PD) interact with putative root-associated pathogens and how these interactions mediate diversity-productivity relationships. We show that (i) both SR and PD were positively correlated with biomass for both adult trees and seedlings across multiple spatial scales, but productivity was best predicted by PD; (ii) significant positive relationships between PD and productivity were observed in nonsterile soil only; and (iii) root fungal diversity was positively correlated with plant PD and SR, while the relative abundance of putative pathogens was negatively related to plant PD. Our findings highlight the key role of soil pathogenic fungi in tree diversity-productivity relationships and suggest that increasing PD may counteract negative effects of plant-soil feedback.

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Section: Discussionsupporting

confidence: 92%

Soil microbes drive phylogenetic diversity-productivity relationships in a subtropical forest

et al. 2019

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“…Long-term replant cropping will lead to an imbalance in the rhizosphere microbial population, reducing beneficial microbes and increasing the abundance of soil-borne fungal pathogens, ultimately leading to a decrease in crop yield ( Mazzola, 1998 ; Yim et al, 2013 ; Franke-Whittle et al, 2015 ; Spath et al, 2015 ). At present, T-RFLP, qPCR, and DNA stable isotope probing (DNA-SIP) techniques are often used to study soil microbial community structure ( Cretoiu et al, 2013 ; Gómez Expósito et al, 2017 ; Moein et al, 2019 ). Shennan et al (2018) found that when rice bran was utilized as the ASD carbon input, significant changes in bacterial and fungal community composition were observed in the soil as characterized by T-RFLP analysis.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

et al. 2021

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Apple replant disease (ARD) is a common problem in major apple planting areas, and biological factors play a leading role in its etiology. Here, we isolated the bacterial strain QSB-6 from the rhizosphere soil of healthy apple trees in a replanted orchard using the serial dilution method. Strain QSB-6 was provisionally identified as Bacillus amyloliquefaciens based on its morphology, physiological and biochemical characteristics, carbon source utilization, and chemical sensitivity. Maximum likelihood analysis based on four gene sequences [16S ribosomal RNA gene (16S rDNA), DNA gyrase subunit A (gyrA), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB)] from QSB-6 and other strains indicated that it had 100% homology with B. amyloliquefaciens, thereby confirming its identification. Flat standoff tests showed that strain QSB-6 had a strong inhibitory effect on Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Fusarium oxysporum, Alternaria alternata, Aspergillus flavus, Phoma sp., Valsa mali, Rhizoctonia solani, Penicillium brasilianum, and Albifimbria verrucaria, and it had broad-spectrum antibacterial characteristics. Extracellular metabolites from strain QSB-6 showed a strong inhibitory effect on Fusarium hyphal growth and spore germination, causing irregular swelling, atrophy, rupture, and cytoplasmic leakage of fungal hyphae. Analysis of its metabolites showed that 1,2-benzenedicarboxylic acid and benzeneacetic acid, 3- hydroxy-, methyl ester had good inhibitory effects on Fusarium, and increased the length of primary roots and the number of lateral roots of Arabidopsis thaliana plantlet. Pot experiments demonstrated that a QSB-6 bacterial fertilizer treatment (T2) significantly improved the growth of Malus hupehensis Rehd. seedlings. It increased root length, surface area, tips, and forks, respiration rate, protective enzyme activities, and the number of soil bacteria while reducing the number of soil fungi. Fermentation broth from strain QSB-6 effectively prevented root damage from Fusarium. terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (qPCR) assays showed that the T2 treatment significantly reduced the abundance of Fusarium in the soil and altered the soil fungal community structure. In summary, B. amyloliquefaciens QSB-6 has a good inhibitory effect on Fusarium in the soil and can significantly promote plant root growth. It has great potential as a biological control agent against ARD.

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“…According to reports, G. sylvaticum can infect more than ten different crops, including apples, carrots, lettuce, and soybeans [34][35][36]. The infection of pumpkin, eggplant, spring onions, and green beans by G. sylvaticum has not been previously reported; however, the infection of these plants was identified from the plant samples used in this study.…”

Section: Host Plants Of G Sylvaticum In Guizhou Chinamentioning

confidence: 76%

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

Zhang

Sun

et al. 2023

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Globisporangium, especially G. sylvaticum, causes devastating root rot, blight, and other diseases in various species of cash crops. To investigate the distribution and host range of G. sylvaticum in Guizhou, a suitable habitat for this pathogen, we collected 156 root-diseased samples, isolated the pathogens, and found that G. sylvaticum is widespread and has eleven host plants, including four novel hosts. Furthermore, to effectively identify G. sylvaticum, we developed a simple and dependable method based on loop-mediated isothermal amplification (LAMP), which used a primer set designed from the internal transcribed spacer sequences with high specificity and sensitivity of 1 pg/μL. Additionally, to perform field identification, we used the “Plant-LAMP” method with crude DNA extraction to detect the pathogen in 45 root samples from nine species of plants. Our results showed that this method could effectively detect G. sylvaticum in diseased roots. Therefore, our findings not only enrich existing research on the diversity of pathogenic Globisporangium in Guizhou but also present an efficient LAMP field detection method that could significantly contribute to plant disease management and prevention.

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Evaluating different approaches for the quantification of oomycete apple replant pathogens, and their relationship with seedling growth reductions

Cited by 15 publications

References 29 publications

Soil microbes drive phylogenetic diversity-productivity relationships in a subtropical forest

Soil microbes drive phylogenetic diversity-productivity relationships in a subtropical forest

Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

Host Range and Loop-Mediated Isothermal Amplification Detection of Globisporangium sylvaticum from Guizhou, China

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