2020
DOI: 10.1007/s00442-020-04743-4
|View full text |Cite
|
Sign up to set email alerts
|

Evaluating environmental DNA as a tool for detecting an amphibian pathogen using an optimized extraction method

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

0
16
0

Year Published

2020
2020
2025
2025

Publication Types

Select...
6
3
1

Relationship

0
10

Authors

Journals

citations
Cited by 14 publications
(16 citation statements)
references
References 43 publications
0
16
0
Order By: Relevance
“…Earlier work showed that Batrachochytrium DNA was persistent in soil for months (Stegen et al 2017). In a comparative field study of DNA from frog skin swabs with environmental DNA from water and sediment samples (collected in the ponds the frogs inhabited), Brannelly et al (2020) found that sediment samples generally had lower Batrachochytrium DNA concentrations than water samples, and limited seasonality relative to the other sample matrices. A logical next step would be to extract a core from a site where longterm data exist on Batrachochytrium infections to evaluate how congruent the sediment archive is with the epidemiological data.…”
Section: Targeted Approaches A) Fishesmentioning
confidence: 98%
“…Earlier work showed that Batrachochytrium DNA was persistent in soil for months (Stegen et al 2017). In a comparative field study of DNA from frog skin swabs with environmental DNA from water and sediment samples (collected in the ponds the frogs inhabited), Brannelly et al (2020) found that sediment samples generally had lower Batrachochytrium DNA concentrations than water samples, and limited seasonality relative to the other sample matrices. A logical next step would be to extract a core from a site where longterm data exist on Batrachochytrium infections to evaluate how congruent the sediment archive is with the epidemiological data.…”
Section: Targeted Approaches A) Fishesmentioning
confidence: 98%
“…Stockwell et al, 2008). Researchers have used water filtration (Hyman & Collins, 2012;Kirshtein et al, 2007) and eDNA detection (Brannelly et al, 2020;Chestnut et al, 2014;Kamoroff & Goldberg, 2017) to screen water samples for Bd DNA. These methods are effective, but are generally limited as they are expensive, time consuming, and require specialized skills and training.…”
Section: Introductionmentioning
confidence: 99%
“…Testing for the presence and concentration of microbial pathogens such as Bd using eDNA methods is appealing because it relies on noninvasive sampling, and free-living stages persistent over several weeks can be detected (Brannelly et al 2020). Similarly, amphibian diversity can be assessed without the need to find and capture animals in the environment, thus avoiding harming animals and introducing sampling biases (Valentini et al 2016).…”
Section: Introductionmentioning
confidence: 99%