RISC-V offers a modular technical approach combined with an open, royalty-free instruction set architecture (ISA). However, despite its advantages as a fundamental building block for many embedded systems, the escalating complexity and functional demands of real-time applications have made adhering to response time deadlines challenging. For real-time applications of RISC-V, real-time performance analysis is required for various ISAs. In this paper, we analyze the real-time performance of RISC-V through two real-time approaches based on processor architectures. For real-time operating system (RTOS) applications, we adopted FreeRTOS and evaluated its performance on HiFive1 Rev B (RISC-V) and STM3240G-EVAL (ARM M). For real-time Linux, we utilized Linux with the Preempt-RT patch and tested its performance on VisionFive 2 (RISC-V), MIO5272 (x86-64), and Raspberry Pi 4 B (ARM A). Through these experiments, we examined the response times on the real-time mechanisms of each operating system. Additionally, in the Preempt-RT experiments, scheduling latencies were evaluated by means of the cyclictest. These are very important parameters for implementing real-time applications comprised of multi-tasking. Finally, in order to show the real-time capabilities of RISC-V practically, we implemented motion control of a six-axis collaborative robot, which was performed on the VisionFive 2. This implementation provided a comparative result of RISC-V’s performance against the x86-64 architecture. Ultimately, the results indicated that the real-time performance of RISC-V for real-time applications was feasible. A noticeable achievement of this research is its first implementation of an EtherCAT master on RISC-V designed for real-time applications. The successful implementation of the EtherCAT master on RISC-V shows real-time capabilities for a wide range of real-time applications.