Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites

Abstract

Abstract:In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K.

Potent Anti-Inflammatory Activity of Pyrenocine A Isolated from the Marine-Derived FungusPenicillium paxilliMa(G)K

“…The CH 2 Cl 2 fraction (130 mg) was separated by HPLC using a C 18 reversed phase column Inertsil ODS-3 (4.6 × 250 mm; 5  μ m) with a gradient of 1 : 1 MeOH : MeCN in H 2 O plus 0.1% formic acid (flow rate: 1.0 mL/min; detection: UV absorption at λ max 254 nm). Pyrenocine A (42.7 mg) was identified by analyzing the spectroscopic data and comparing them with the data in the literature [18]. …”

Biotransformation of methyl parathion by marine-derived fungi isolated from ascidian Didemnum ligulum

“…Mirna H. Regali Seleghim. The methodology for the ascidian collection, isolation and preservation of fungi strains are described in Kossuga et al, (2012).…”

Effect of cultural conditions on antimicrobial activity of marine-derived fungus Penicillium chrysogenum

“…In fact, culture conditions have a major impact on the growth of microbes and the production of microbial products. As far as culture conditions are concerned, there is usually a dilemma between achieving maximal growth rates and maximal antibiotic yields because conditions that allow fast cell growth could be unfavorable to metabolite production (Fiedurek et al, 1996;Miao et al, 2006;Xu et al, 2008;Mohanty, Prakash, 2009;Kossuga et al, 2012). Changes in pH, temperature, incubation period, shaking and inoculum size of the antagonistic fungal strain can greatly influence antibiotic biosynthesis (Calvo et al, 2002;Llorens et al, 2004).…”