Evaluating optical coherence tomography for surgical margin assessment of canine mammary tumours

Fabelo, Carolina; Selmic, Laura E.; Huang, Pin‐Cheh; Samuelson, Jonathan; Reagan, Jennifer K.; Kalamaras, A; Wavreille, Vincent A; Monroy, Guillermo L.; Marjanović, Marina; Boppart, Stephen A.

doi:10.1111/vco.12632

Cited by 6 publications

(14 citation statements)

References 33 publications

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“…Surgery is the primary treatment in the control of CMTs; the goal is to remove the tumor(s) with clean margins and, depending on the case, to prevent the development of new tumors in the remaining glands ( 4 ). Clean margins have been found to be predictive of the median survival time (MST) in dogs with stages I–III ( 19 ), and very recent publications have elucidated new strategies for the intraoperative assessment of margins using near-infrared light waves to generate real-time, high-resolution images on the microscopic scale, similar to low-power histopathology ( 47 – 49 ).…”

Section: Conventional and New Clinical And Histological Approachesmentioning

confidence: 99%

From Conventional to Precision Therapy in Canine Mammary Cancer: A Comprehensive Review

et al. 2021

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Canine mammary tumors (CMTs) are the most common neoplasm in intact female dogs. Canine mammary cancer (CMC) represents 50% of CMTs, and besides surgery, which is the elective treatment, additional targeted and non-targeted therapies could offer benefits in terms of survival to these patients. Also, CMC is considered a good spontaneous intermediate animal model for the research of human breast cancer (HBC), and therefore, the study of new treatments for CMC is a promising field in comparative oncology. Dogs with CMC have a comparable disease, an intact immune system, and a much shorter life span, which allows the achievement of results in a relatively short time. Besides conventional chemotherapy, innovative therapies have a large niche of opportunities. In this article, a comprehensive review of the current research in adjuvant therapies for CMC is conducted to gather available information and evaluate the perspectives. Firstly, updates are provided on the clinical–pathological approach and the use of conventional therapies, to delve later into precision therapies against therapeutic targets such as hormone receptors, tyrosine kinase receptors, p53 tumor suppressor gene, cyclooxygenases, the signaling pathways involved in epithelial–mesenchymal transition, and immunotherapy in different approaches. A comparison of the different investigations on targeted therapies in HBC is also carried out. In the last years, the increasing number of basic research studies of new promising therapeutic agents on CMC cell lines and CMC mouse xenografts is outstanding. As the main conclusion of this review, the lack of effort to bring the in vitro studies into the field of applied clinical research emerges. There is a great need for well-planned large prospective randomized clinical trials in dogs with CMC to obtain valid results for both species, humans and dogs, on the use of new therapies. Following the One Health concept, human and veterinary oncology will have to join forces to take advantage of both the economic and technological resources that are invested in HBC research, together with the innumerable advantages of dogs with CMC as a spontaneous animal model.

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Section: Conventional and New Clinical And Histological Approachesmentioning

confidence: 99%

From Conventional to Precision Therapy in Canine Mammary Cancer: A Comprehensive Review

et al. 2021

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“…To date, OCT imaging has been evaluated in a limited capacity for surgical margin assessment in dogs. 5,21,22,40,41 Initial studies demonstrated differences in microstructural appearance and textural characteristics of different tissues at surgical margins. 5,21,22,40,41 The microstructural features were used to train observers of different specialties (radiology, pathology, surgery and OCT imaging) in a recent study.…”

Section: Discussionmentioning

confidence: 99%

“…5,21,22,40,41 Initial studies demonstrated differences in microstructural appearance and textural characteristics of different tissues at surgical margins. 5,21,22,40,41 The microstructural features were used to train observers of different specialties (radiology, pathology, surgery and OCT imaging) in a recent study. 40 Observers showed good accuracy of interpretation for ex vivo images, with an overall sensitivity of 83% and specificity of…”

Section: Discussionmentioning

confidence: 99%

Intraoperative assessment of canine soft tissue sarcoma by deep learning enhanced optical coherence tomography

Sun

et al. 2021

Vet Comparative Oncology

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Soft tissue sarcoma (STS) is a locally aggressive and infiltrative tumour in dogs. Surgical resection is the treatment of choice for local tumour control. Currently, postoperative pathology is performed for surgical margin assessment. Spectral-domain optical coherence tomography (OCT) has recently been evaluated for its value for surgical margin assessment in some tumour types in dogs. The purpose of this study was to develop an automatic diagnosis system that can assist clinicians in real-time for OCT image interpretation of tissues at surgical margins. We utilized a ResNet-50 network to classify healthy and cancerous tissues. A patch-based approach was adopted to achieve accurate classification with limited training data (80 cancer images, 80 normal images) and the validation set (20 cancer images, 20 normal images). The proposed method achieved an average accuracy of 97.1% with an excellent sensitivity of 94.3% on the validation set; the quadratic weighted κ was 0.94 for the STS diagnosis. In an independent test data set of 20 OCT images (10 cancer images, 10 normal images), the proposed method correctly differentiated all the STS images. Furthermore, we proposed a diagnostic curve, which could be evaluated in real-time to assist clinicians in detecting the specific location of a lesion. In short, the proposed method is accurate, operates in real-time and is non-invasive, which could be helpful for future surgical guidance.

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“…Conversely, OCT usually operates in reflection mode, and is well suited to imaging thick tissue specimens. OCT has shown potential in label-free tissue block imaging 11 , 24 , surgical margin assessment 25 , 26 , and biopsy examination 27 , 28 . However, OCT uses optical scattering contrast which highlights predominately morphological information.…”

Section: Introductionmentioning

confidence: 99%

Virtual histological staining of label-free total absorption photoacoustic remote sensing (TA-PARS)

Boktor

Ecclestone

Pekar

et al. 2022

Sci Rep

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Histopathological visualizations are a pillar of modern medicine and biological research. Surgical oncology relies exclusively on post-operative histology to determine definitive surgical success and guide adjuvant treatments. The current histology workflow is based on bright-field microscopic assessment of histochemical stained tissues and has some major limitations. For example, the preparation of stained specimens for brightfield assessment requires lengthy sample processing, delaying interventions for days or even weeks. Therefore, there is a pressing need for improved histopathology methods. In this paper, we present a deep-learning-based approach for virtual label-free histochemical staining of total-absorption photoacoustic remote sensing (TA-PARS) images of unstained tissue. TA-PARS provides an array of directly measured label-free contrasts such as scattering and total absorption (radiative and non-radiative), ideal for developing H&E colorizations without the need to infer arbitrary tissue structures. We use a Pix2Pix generative adversarial network to develop visualizations analogous to H&E staining from label-free TA-PARS images. Thin sections of human skin tissue were first virtually stained with the TA-PARS, then were chemically stained with H&E producing a one-to-one comparison between the virtual and chemical staining. The one-to-one matched virtually- and chemically- stained images exhibit high concordance validating the digital colorization of the TA-PARS images against the gold standard H&E. TA-PARS images were reviewed by four dermatologic pathologists who confirmed they are of diagnostic quality, and that resolution, contrast, and color permitted interpretation as if they were H&E. The presented approach paves the way for the development of TA-PARS slide-free histological imaging, which promises to dramatically reduce the time from specimen resection to histological imaging.

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Evaluating optical coherence tomography for surgical margin assessment of canine mammary tumours

Cited by 6 publications

References 33 publications

From Conventional to Precision Therapy in Canine Mammary Cancer: A Comprehensive Review

From Conventional to Precision Therapy in Canine Mammary Cancer: A Comprehensive Review

Intraoperative assessment of canine soft tissue sarcoma by deep learning enhanced optical coherence tomography

Virtual histological staining of label-free total absorption photoacoustic remote sensing (TA-PARS)

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