Evaluating the antidiabetic effects of Chinese herbal medicine: Xiao-Ke-An in 3T3-L1 cells and KKAy mice using both conventional and holistic omics approaches

Yang, Zhenzhong; Wang, Linli; Zhang, Feng; Li, Zheng

doi:10.1186/s12906-015-0785-2

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…By manually text-mining the published literatures in Pubmed, the anti-CCVD effects of these constituents in XST were summarized. The anti-CCVD effect was evaluated by Reverse Rate (RR) 48 , 49 , which was calculated by equation ( 1 ). where N j , M j and T j indicate the performances of normal, model and constituent treated group in report j .…”

Section: Methodsmentioning

confidence: 99%

A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study

Yang

Shao

et al. 2017

Sci Rep

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Current chemical markers based quality assessment methods largely fail to reflect intrinsic chemical complexity and multiple mechanisms of action of botanical drugs (BD). The development of novel quality markers is greatly needed. Here we propose bioactive chemical markers (BCM), defined as a group of chemo-markers that exhibit similar pharmacological activities comparable to the whole BD, which can therefore be used to effectively assess the quality of BD. As a proof-of-concept, a BCM-based strategy was developed and applied to Xuesaitong Injection (XST) for assessing the efficacy and consistency of different batches. Firstly, systemic characterization of chemical profile of XST revealed a total number of 97 compounds. Secondly, notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and Rd were identified as BCM of XST on treating cardiovascular and cerebrovascular diseases according to Adjusted Efficacy Score following an in vivo validation. Analytical method for quantification of BCM was then developed to ensure the efficacy of XST. Finally, chemical fingerprinting was developed and used to evaluate the batch-to-batch consistency. Our present case study on XST demonstrates that BCM-based strategy offers a rational approach for quality assessment of BD and provides a workflow for chemistry, manufacturing, and controls (CMC) study of BD required by regulatory authority.

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Section: Methodsmentioning

confidence: 99%

A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study

Yang

Shao

et al. 2017

Sci Rep

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“…The neologism “-omics,” including genomics, proteomics, and metabolomics, refers to the collective characterization and quantification of pools of biological molecules that translate into the structure, function, and dynamics of an organism ( Kedaigle and Fraenkel, 2018 ). The current “-omics” studies provide new opportunities for the potential future development of the CHM ( Yang et al, 2015 ). In the last decade, significant developments have been achieved in epigenomics studies, focusing on the complete set of epigenetic modifications.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic Studies of Chinese Herbal Medicine: Pleiotropic Role of DNA Methylation

Guo

Wang

et al. 2021

Front. Pharmacol.

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Accumulating knowledge has been achieved on DNA methylation participating in numerous cellular processes and multiple human diseases; however, few studies have addressed the pleiotropic role of DNA methylation in Chinese herbal medicine (CHM). CHM has been used worldwide for the prevention and treatment of multiple diseases. Newly developed epigenetic techniques have brought great opportunities for the development of CHM. In this review, we summarize the DNA methylation studies and portray the pleiotropic role of DNA methylation in CHM. DNA methylation serves as a mediator participating in plant responses to environmental factors, and thus affecting CHM medicinal plants growth and bioactive compound biosynthesis which are vital for therapeutic effects. Furthermore, DNA methylation helps to uncover the pharmaceutical mechanisms of CHM formulae, herbs, and herbal-derived compounds. It also provides scientific validation for constitution theory and other essential issues of CHM. This newly developed field of DNA methylation is up-and-coming to address many complicated scientific questions of CHM; it thus not only promotes disease treatment but also facilitates health maintenance.

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“…Studies have shown that Ber offers potential for the treatment of type 2 diabetes mellitus (T2DM) (2-7), whereas CCE is primarily one of the components of a Chinese traditional formula for the treatment of diabetes mellitus (DM) via the prevention of associated vascular and cardiac damage, renal fibrosis and lipid metabolism (8)(9)(10). Ber is a classical natural medicine, the applications of which have attracted interest in investigations focused on the treatment of DM (11)(12)(13)(14)(15). The pharmacological effects of Ber on glucose metabolism…”

Section: Introductionmentioning

confidence: 99%

Protective role of berberine and Coptischinensis extract on T2MD rats and associated islet Rin-5f cells

Jiang

Cui

Wang

et al. 2017

Molecular Medicine Reports

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The aim of the present study was to compare the different effects of berberine (Ber) and Coptischinensis extract (CCE) on a rat model of type 2 diabetes mellitus (T2DM), and the islet Rin‑5f cell line was used to examine the differences between Ber and CCE and the underlying mechanisms. CCE was extracted and purified prior to analysis. Male Sprague‑Dawley rats were provided with a high‑fat diet to induce insulin resistance prior to injecting with streptozotocinto establish the T2DM model, the T2DM rats were treated with Ber and CCE, and blood samples and pancreatic tissues were obtained and compared to examine T2DM metabolic syndromes among the groups of rats, which included healthy rats, model rats, and model rats treated with Ber and CCE at different doses between 0 and 8 weeks. The protective effects of Ber and CCE on the Rin‑5f islet cell line were also evaluated. The effects on Rin‑5f cell proliferation and cell cycle, glucose‑stimulated insulin release test (GSIS), the anti‑apoptotic effects caused by fat induction, and protein expression levels of poly ADP‑ribose polymerase (PARP‑1) were evaluated. The results showed that the content of the prepared CCE was 96.07% for five alkaloids. When it was used for treatment of the T2DM rats, compared with Ber, metformin and rosiglitazone, the fasting blood glucose, glucosylated serum protein (GSP) and glucose infusion rate indicesin the fasting rats were ameliorated, compared with those in the T2MD rats, with no significant differences between treatment with Ber or CCE and metformin or rosiglitazone. The indices of mean optical density and fasting β‑cell function index (FBCI) were different following treatment with Ber and CCE, compared with those in the model rats, which may have stimulated the pancreatic secretion of insulin. When Ber and CCE were used to examine the protective effects on Rin‑5F cells, it was found that the Rin‑5f cell GSIS, cell cycle, lipotoxic islet cell proliferation and protein expression of PARP‑1 were altered and improved, which may have protected pancreatic islet β‑cells by improving islet β‑cell proliferation and the protein expression of PARP‑1. CCE and Ber exerted similar effects when used for the treatment of T2MD rats, and may have stimulated the pancreatic secretion of insulin through the protective effect on islet β‑cells via improving islet β‑cell proliferation and the protein expression of PARP‑1.

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Evaluating the antidiabetic effects of Chinese herbal medicine: Xiao-Ke-An in 3T3-L1 cells and KKAy mice using both conventional and holistic omics approaches

Cited by 9 publications

References 24 publications

A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study

A Bioactive Chemical Markers Based Strategy for Quality Assessment of Botanical Drugs: Xuesaitong Injection as a Case Study

Epigenetic Studies of Chinese Herbal Medicine: Pleiotropic Role of DNA Methylation

Protective role of berberine and Coptischinensis extract on T2MD rats and associated islet Rin-5f cells

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