This study aims to prevail the digital information literacy skills among library and information professionals working in different universities of Sindh, Pakistan. For data collection, survey method, and a questionnaire comprised of close-ended questions were used. The population of the study consisted of 138 library professionals working in different public and private universities and institutes of Sindh, a province of Pakistan. The results of study show that the greater part of respondents had good digital information literacy skills regarding the use, understanding, evaluation and communication the digital information. With this, majority of the participants were either self-taught or learn through conferences, workshops, friends and colleagues. The barriers in obtaining digital information literacy skills were found; lack of funds, un-updated LIS curriculum, tough working routine, lack of support from higher authorities. Moreover, this study provides an understanding as to how universities and institutes can strength and up-skill their library professionals.