Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples

He, Maozhang; Fang, Shouguo; Huang, Xiaowan; Zhao, Yuanzhang; Ke, Shanlin; Yang, Hui; Li, Zhuojun; Gao, Jun; Chen, Congying; Huang, Lusheng

doi:10.3389/fmicb.2016.02108

Cited by 56 publications

(49 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, a higher abundance of Bacteroides spp., Lachnospiraceae spp., and Ruminococcacecae spp. was linked to obese animals [57][58][59]. All these arguments are in line with the better performances observed at the end of the experimental period in our experiment.…”

Section: Colonic Microbiome Analysis By 16s Rrna Profilingsupporting

confidence: 92%

Effect of increasing levels of rice distillers’ by-product on growth performance, nutrient digestibility, blood profile and colonic microbiota of weaned piglets

Cong

Taminiau

Kim

et al. 2020

Asian-Australas J Anim Sci

View full text Add to dashboard Cite

Objective: This study was conducted to evaluate the effects of diets containing different wet rice distillers’ by-product (RDP) levels on growth performance, nutrient digestibility, blood profiles and gut microbiome of weaned piglets.Methods: A total of 48 weaned castrated male crossbred pigs, initial body weight 7.54±0.97 kg, and age about 4 wks, were used in this experiment. The piglets were randomly allocated into three iso-nitrogenous diet groups that were fed either a control diet, a diet with 15% RDP, or a diet with 30% RDP for a total of 35 days. Chromium oxide was used for apparent digestibility measurements. On d 14 and d 35, half of the piglets were randomly selected for hemato-biochemical and gut microbiota evaluations.Results: Increasing inclusion levels of RDP tended to linearly increase (p≤0.07) average daily gain on d 14 and d 35, and decreased (p = 0.08) feed conversion ratio on d 35. Empty stomach weight increased (p = 0.03) on d 35 while digestibility of diet components decreased. Serum globulin concentration decreased on d 14 (p = 0.003) and red blood cell count tended to decrease (p = 0.06) on d 35, parallel to increase RDP levels. Gene amplicon profiling of 16S rRNA revealed that the colonic microbiota composition of weaned pigs changed by inclusion of RDP over the period. On d 14, decreased proportions of Lachnospiraceae_ge, Ruminococcaceae_ge, Ruminococcaceae_UCG-005, and Bacteroidales_ge, and increased proportions of Prevotellaceae_ge, Prevotella_2, and Prevotella_9 were found with inclusion of RDP, whereas opposite effect was found on d 35. Additionally, the proportion of Lachnospiraceae_ge, Ruminococcaceae_ge, Ruminococcaceae_UCG-005, and Bacteroidales_ge in RDP diets decreased over periods in control diet but increased largely in diet with 30% RDP.Conclusion: These results indicate that RDP in a favorable way modulate gastrointestinal microbiota composition and improve piglet performance despite a negative impact on digestibility of lipids and gross energy.

show abstract

Section: Colonic Microbiome Analysis By 16s Rrna Profilingsupporting

confidence: 92%

Effect of increasing levels of rice distillers’ by-product on growth performance, nutrient digestibility, blood profile and colonic microbiota of weaned piglets

Cong

Taminiau

Kim

et al. 2020

Asian-Australas J Anim Sci

View full text Add to dashboard Cite

show abstract

“…The α-diversity of microbial community of cecum lumen and feces was described in our previous publication [17]. Here we focused on the identification of bacterial genera enriched in cecum lumen and feces, respectively.…”

Section: Resultsmentioning

confidence: 99%

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

Fang

Xiong

et al. 2017

BMC Microbiol

Self Cite

View full text Add to dashboard Cite

BackgroundIntramuscular fat (IMF) that deposits among muscle fibers or within muscle cells is an important meat quality trait in pigs. Previous studies observed the effects of dietary nutrients and additives on improving the pork IMF. Gut microbiome plays an important role in host metabolism and energy harvest. Whether gut microbiota exerts effect on IMF remains unknown.ResultsIn this study, we investigated the microbial community structure of 500 samples from porcine cecum and feces using high-throughput 16S rRNA gene sequencing. We found that phylogenetic composition and potential function capacity of microbiome varied between two types of samples. Bacteria wide association study identified 119 OTUs significantly associated with IMF in the two types of samples (FDR < 0.1). Most of the IMF-associated OTUs belong to the bacteria related to polysaccharide degradation and amino acid metabolism (such as Prevotella, Treponema, Bacteroides and Clostridium). Potential function capacities related to metabolisms of carbohydrate, energy and amino acids, cell motility, and membrane transport were significantly associated with IMF content. FishTaco analysis suggested that the shifts of potential function capacities of microbiome associated with IMF might be caused by the IMF-associated microbial taxa.ConclusionsThis study firstly evaluated the contribution of gut microbiome to porcine IMF content. The results presented a potential capacity for improving IMF through modulating gut microbiota.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1055-x) contains supplementary material, which is available to authorized users.

show abstract

“…The contribution of microbiome to lung lesion was similarly estimated as described previously (Fu et al, 2015;He et al, 2016). We split the data randomly into a 70% discovery set and a 30% validation set.…”

Section: Estimating the Contribution Of Lung Microbiome To Lung Lesionsmentioning

confidence: 99%

Microbial communities in swine lungs and their association with lung lesions

Huang

Zhang

Tong

et al. 2018

Microbial Biotechnology

Self Cite

View full text Add to dashboard Cite

Summary Under natural farming, environmental pathogenic microorganisms may invade and affect swine lungs, further resulting in lung lesions. However, few studies on swine lung microbiota and their potential relationship with lung lesions were reported. Here, we sampled 20 pigs from a hybrid herd raised under natural conditions; we recorded a lung‐lesion phenotype and investigated lung microbial communities by sequencing the V3‐V4 region of 16S rRNA gene for each individual. We found reduced microbial diversity but more biomass in the severe‐lesion lungs. Methylotenera , Prevotella , Sphingobium and Lactobacillus were the prominent bacteria in the healthy lungs, while Mycoplasma , Ureaplasma , Sphingobium , Haemophilus and Phyllobacterium were the most abundant microbes in the severe‐lesion lungs. Notably, we identified 64 lung‐lesion‐associated OTU s, of which two classified to Mycoplasma were positively associated with lung lesions and 62 showed negative association including thirteen classified to Prevotella and six to Ruminococcus . Cross‐validation analysis showed that lung microbiota explained 23.7% phenotypic variance of lung lesions, suggesting that lung microbiota had large effects on promoting lung healthy. Furthermore, 22 KEGG pathways correlated with lung lesions were predicted. Altogether, our findings improve the knowledge about swine lung microbial communities and give insights into the relationship between lung microbiota and lung lesions.

show abstract

Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples

Cited by 56 publications

References 75 publications

Effect of increasing levels of rice distillers’ by-product on growth performance, nutrient digestibility, blood profile and colonic microbiota of weaned piglets

Effect of increasing levels of rice distillers’ by-product on growth performance, nutrient digestibility, blood profile and colonic microbiota of weaned piglets

16S rRNA gene-based association study identified microbial taxa associated with pork intramuscular fat content in feces and cecum lumen

Microbial communities in swine lungs and their association with lung lesions

Contact Info

Product

Resources

About