Evaluating the Effect of Force Feedback in Cell Injection

Pillarisetti, Anand; Pekarev, Maxim; Brooks, Ari D.; Desai, Jaydev P.

doi:10.1109/tase.2006.888051

Cited by 117 publications

(52 citation statements)

References 24 publications

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“…This approach is crucial to addressing the fundamental analysis of cellular functions, mechanisms in living systems, genetic transformation, and cell-based assays [2]. Microinjection is one of the most widely used techniques for delivering biomolecules [6,7]. In this method, a glass micropipette with a diameter of around 1 µm is used to penetrate a cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Intracellular delivery method based on a combination of electrokinetic forces and vibration-assisted cell membrane perforation

Shibata

Ito

Ozawa

et al. 2014

AIP Conference Proceedings

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Abstract. The introduction of biological macromolecules such as DNA, RNA, and proteins into living cells plays a crucial role in the fundamental analysis of cellular functions and mechanisms in living systems. Therefore, we have been developing an effective platform for the in vitro manipulation and analysis of biological cells at the single-cells. In this paper, we successfully demonstrated a novel intracellular delivery method of DNA into living HeLa cells via a glass micropipette based on DC-biased AC electrokinetically driven forces. We also proposed a vibration-assisted insertion method for penetrating a cell membrane to reduce cell damage. Preliminary insertion tests on homemade SICM system and FEM simulations revealed that the application of the mechanical oscillation can reduce the deformation of cells probably due to an increase in their viscous resistance. Moreover, we also found that a change in the ion current during the insertion process allows us to detect the instant when the micropipette tip penetrates the cell membrane.

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Section: Introductionmentioning

confidence: 99%

Intracellular delivery method based on a combination of electrokinetic forces and vibration-assisted cell membrane perforation

Shibata

Ito

Ozawa

et al. 2014

AIP Conference Proceedings

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“…Various cell injection systems have been developed to provide more controllable manipulation of biological cells [2], [3], [4]. If visual servoing is a necessary condition for accurate micro-injection operations, the knowledge of interactive forces acting during pipette insertion plays an important role too since it can be used to provide force feedback for precise regulation of needle penetration speed and strength [5]. Reducing cell deformation and needle deviation will also lower the risk of cell damage.…”

Section: Introductionmentioning

confidence: 99%

“…Reducing cell deformation and needle deviation will also lower the risk of cell damage. However, when manipulating deformable biological objects, force sensor measurement will provide only the local forces at the pipette puncture point which limits strongly the operator haptic rendering [5]. In order to learn, train and analyze basic cell injection procedures, most operators are aiming to use artificial cells because practicing on human oocyte has ethical concerns and potential risks [6].…”

Section: Introductionmentioning

confidence: 99%

Micro-to-Nano Biomechanical Modeling for Assisted Biological Cell Injection

Ladjal

Hanus

Ferreira

2013

IEEE Trans. Biomed. Eng.

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Abstract-To facilitate training of biological cell injection operations, we are developing an interactive virtual environment to simulate needle insertion into biological cells. This paper presents methodologies for dynamic modeling, visual/haptic display and model validation of cell injection. We first investigate the challenging issues in the modeling of the biomechanical properties of living cells. We propose two dynamic models to simulate cell deformation and puncture. The first approach is based on the assumptions that the mechanical response of living cells is mainly determined by the cytoskeleton and that the cytoskeleton is organized as a tensegrity structure including microfilaments, microtubules and intermediate filaments.Equivalent microtubules struts are represented with a linear mass-tensor finite element model and equivalent microfilaments and intermediate filaments with viscoelastic Kelvin-Voigt elements. The second modeling method assumes the overall cell as an homogeneous hyperelastic model (St-Venant-Kirchhoff). Both graphic and haptic rendering are provided in real-time to the operator through a 3D virtual environment. Simulated responses are compared to experimental data to show the effectiveness of the proposed physically-based model.

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“…Consequently, there is a need for quantification of forces during biological cell injection and for an automated cell injection system. Providing force feedback to the operator will improve the success rate of the injection task (2). Teleoperation technology has also been investigated for the development of emerging microsurgery systems (3,4).…”

Section: Introductionmentioning

confidence: 99%

Development of a piezo‐actuated micro‐teleoperation system for cell manipulation

Zareinejad

Rezaei

Abdullah

et al. 2009

Robotics Computer Surgery

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Background Intracytoplasmic sperm injection (ICSI) requires long training and has low success rates, primarily due to poor control over the injection force. Making force feedback available to the operator will improve the success rate of the injection task. A macro-micro-teleoperation system bridges the gap between the task performed at the micro-level and the macroscopic movements of the operator. The teleoperation slave manipulator should accurately position a needle to precisely penetrate a cell membrane. Piezoelectric actuators are widely used in micromanipulation applications; however, hysteresis non-linearity limits the accuracy of these actuators.

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Evaluating the Effect of Force Feedback in Cell Injection

Cited by 117 publications

References 24 publications

Intracellular delivery method based on a combination of electrokinetic forces and vibration-assisted cell membrane perforation

Intracellular delivery method based on a combination of electrokinetic forces and vibration-assisted cell membrane perforation

Micro-to-Nano Biomechanical Modeling for Assisted Biological Cell Injection

Development of a piezo‐actuated micro‐teleoperation system for cell manipulation

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