Evaluating the Efficiency of Entomopathogenic Fungi Against the Asian Longhorned Beetle,Anoplophora glabripennis(Coleoptera: Cerambycidae), by Using Cages in the Field

Environmental contamination withMetarhizium anisopliaefrom fungal bands for control of the Asian longhorned beetle,Anoplophora glabripennis(Coleoptera: Cerambycidae)

“…Significant reductions in adult A. glabripennis longevity, in fungal-treated compared with control plots, were observed for most strains tested. A sublethal effect was also observed in the field, as fungal band treatments resulted in reduced oviposition (Dubois et al 2004a;Dubois, Li, Jiafu and Hajek 2004b;Hajek, Huang, Dubois, Smith and Li 2006). Oviposition reduction as a sublethal effect of fungal bands has been shown in the past for other tree-boring cerambycids (Ninomiya and Higuchi 1998;Tsutsumi 1998), and contributes to the efficacy of this method of control.…”

“…Under favorable conditions, fungal bands retain infectivity in the field for over 40 days (Higuchi et al 1997;Matsuura, Fukui and Adachi 1997). In contrast, conidial viability is significantly reduced 10 days after spraying fungal spores onto tree trunks (Dubois, Hajek, Jiafu and Li 2004a). Bands can be stored at low temperatures for long periods of time (400 days at 58C), and conidial viability of dried band cultures can be restored by adding water (Higuchi et al 1997).…”

Microbial control of wood-boring insects attacking forest and shade trees

“…Trials began with a comparison of adults caged with tree trunks that had been sprayed with either B. bassiana or B. brongniartii or caged with fungal bands of these fungal species (Dubois et al, 2004a) (Figure 3). Longevity and oviposition of adults receiving the two treatments were similar.…”

“…To produce fungal bands, fungal cells are grown in liquid media on shakers (Dubois et al, 2004a). Once cell number is maximal, additional media and molten agar are added.…”

Desenvolvimento de armadilha de auto-inoculação para o controle de Hypothenemus hampei (Ferrari, 1867) (Coleoptera: Curculionidae) com Beauveria bassiana (Bals.) Vuil (Ascomycota: Hypocreales) em tecido sintético

“…As pupas inicialmente são brancas se tornando amareladas com a formação dos apêndices do inseto (FERNÁNDEZ;CORDERO, 2007 (BRUN et al 1989;BRUN et al, 1990 Cerambycidae), obtendo resultados satisfatórios (DUBOIS et. al., 2004a;2004b;HAJEK et. al., 2006;HAJEK, 2008;SHANLEY et.…”