Evaluating the efficiency of humic acid to remove micro‐organisms from denture base material

Meriç, Gökçe; Güvenir, Meryem; Süer, Kaya

doi:10.1111/ger.12175

Cited by 4 publications

(4 citation statements)

References 37 publications

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“…C. albicans , S . aureus , E. faecalis (gram‐positive) and P. aeruginosa (gram‐negative) are some of the microorganisms evaluated from the microbiota of old complete dentures and that were also found in implant‐supported fixed complete dentures from our study 30,31 …”

Section: Discussionmentioning

confidence: 59%

“…30 C. albicans, S. aureus, E. faecalis (gram-positive) and P. aeruginosa (gram-negative) are some of the microorganisms evaluated from the microbiota of old complete dentures and that were also found in implant-supported fixed complete dentures from our study. 30,31 The observed Candida genera did not vary among the evaluated patients: Candida albicans was the most verified species checkerboard method. In some studies, C. albicans was the main species isolated in complete denture wearers, 27,32 followed by Candida tropicalis.…”

Section: Discussionmentioning

confidence: 85%

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Clinical study of the biofilm of implant‐supported complete dentures in healthy patients

et al. 2021

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Objective The purpose of this study was to quantify the area covered by biofilm and identify bacteria and yeasts present in mandibular acrylic resin full‐arch implant‐supported fixed prostheses. Background Biofilm control of implant‐supported fixed prosthesis is hampered by their design, and it can cause oral and systemic problems, mainly in immunocompromised patients like the elder. Knowledge about microbiota reinforces the awareness about the need for periodic professional cleaning maintenance. Materials and methods Twenty prostheses were unscrewed, washed in 0.89% sodium chloride, stained with eosin 1% and photographed. The area covered by biofilm was digitally delimited and quantified. Biofilm samples were collected, diluted up to 1:107, seeded in chromogenic agar media and incubated for 48 hours, at 37°C, for counting of colony‐forming units (CFU/mL). DNA hybridization was performed to complement the identification and quantification of microorganisms. Data were analyzed using Mann‐Whitney test, Spearman correlation and Fisher's exact test (α = .05). Results An average of 62% of the gingival surface of the prostheses was covered by biofilm. Enterococcus spp. (5.82 ± 1.38 log10CFU/mL) and Staphylococcus aureus (5.75 ± 2.02 log10CFU/mL) showed higher prevalence in cultures. Patients with five implants had less biofilm compared to those with four implants (P = .031) but had higher Escherichia coli counts (P = .039). In DNA hybridization, Streptococcus pneumoniae, Veillonella parvula and Fusobacterium nucleatum presented higher quantification and were present in all the samples; patients over 65 years old contained more Candida tropicalis (P = .049); prostheses on five implants presented lower quantification for several species. Conclusion Biofilm was present on all prostheses, containing potentially pathogenic microorganisms. The number of implants may play a role in quantification of biofilm and in microorganism counts.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 85%

Clinical study of the biofilm of implant‐supported complete dentures in healthy patients

et al. 2021

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“…The previous study [14] showed that humic acid solution significantly reduced the microorganisms on dentures. However, beside the effectiveness of the introduced experimental solution, the side effects of the solution, such as discoloration or surface deterioration of dentures, were not evaluated in the study.…”

Section: Discussionmentioning

confidence: 94%

“…Humic acid has recently been introduced as an alternative denture-cleaning agent. The effect of humic acid to remove microorganisms from denture base material was reported [14] . Humus is that organic portion of soil that remains after millions of years of microbial decomposition of plants and vegetation.…”

Section: Evaluating the Efficiency Of Humic Acid To Remove Stain Frommentioning

confidence: 99%

Evaluating the Efficiency of Humic Acid to Remove Stain form Artificial Teeth

Merä°Ã‡¹,

Aktore²,

Ongun³

et al. 2015

JDOC

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Objective: to assess the color stability of artificial teeth after immersion in different staining solutions and to determine the effect humic acid on removing stain from teeth. Background: Old denture wearer needs effective, easy-use and safe denture-cleaning material. Material and Methods: Sixty maxillary incisors were divided into the three groups according to staining solution as tea (T), cherry juice (CJ) and cola (C). Baseline color measurements were performed with a spectrophotometer. Specimens were immersedin a staining solutions for 14 h (2 h _ 7 days) and then second color measurements were performed. Each group was further divided into four subgroups according to denture cleanser as Corsodyl (Cr), Corega tablet (Ct), humic acid (H) and distilled water (W) (n=5). Specimens were immersed in denture cleansers for 8h and third color measurements were made. Thus, the weekly simulation period was completed. This cycle was repeated 12 times to simulate a 3-month time period and measurements were performed. Color differences were calculated and data were analyzed with 2-way repeated measures ANOVA and Tukey tests. Results: The highest color difference after staining was noted in T. After the 12-times repeated immersion beverage and cleanser cycle, discoloration of teeth stained in beverages and cleaned with different solutionswas demonstrated. H and W were more effective than others for the teeth stained in T and Cj. However there was no difference among the cleaning solutions for teeth stained in C. Conclusions: Humic acid could be used as an alternative "natural" solution for denture-cleaning agent.

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Antifungal effect of tea extracts on <i>Candida albicans </i>

Huang

Hong

et al. 2020

Dent. Mater. J.

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Removable dentures are a common treatment for complete and partial tooth loss 1,2). Owing to decreased dexterity with age, the majority of elderly people with dentures fail to keep their denture clean and generally have poor oral hygiene 3). Denture-related stomatitis (atrophic chronic candidiasis), an inflammatory lesion of the denture-bearing mucosa, is the most common oral fungal infection in denture wearers 4,5). Although there may be systemic factors related to denture-related stomatitis, microbial plaques that form on the denture surface have been considered a critical factor favoring denture-related stomatitis 6). Candida is a commensal fungus that inhabits the oral cavity in approximately 50% of people, and Candida albicans (C. albicans) was found in 81.7% of denture plaques 7,8). Thus, denturerelated stomatitis is, at least to some extent, attributed to the proliferation of C. albicans in plaques attached to the dentures. Currently, there are 3 methods for removing plaque and debris from dentures: mechanical, chemical, and thermal methods 9). The mechanical methods include the use of denture brushes and ultrasonic devices. As the most widely used mechanical method, brushing with a soft denture brush and formulated toothpaste may be able to remove C. albicans biofilms from the dentures 10). However, the inappropriate motion of the denture brush, especially when using toothpaste, might scratch the dentures and cause surface irregularities, which could further accelerate adherence of the microflora 11). The chemical method involves immersion of the denture in chemical solutions, such as alkaline peroxides, sodium hypochlorite, acids, enzymes, and neutral enzymatic peroxides solutions, for a certain period of time 12). Vieira et al. 13) compared the effects of alkaline peroxides, 0.5% sodium hypochlorite (NaClO) and distilled water on the removal of C. albicans biofilms in a laboratory study. The results suggested that NaClO was the only treatment that removed biofilms efficiently. However, NaClO may corrode metal and alter color, increase the surface roughness, reduce the flexural strength and cause color changes in acrylic resins 14-16). In addition, such solutions may have a disagreeable smell and taste for patients 17). Recently, a thermal method that uses microwave energy and ultraviolet C light ovens has been proven effective in denture cleaning 9). As a newly established thermal cleaning method, the use of microwave energy was found to be useful in removing C. albicans biofilms from dentures. However, dentures are likely to undergo harmful dimensional changes, particularly after repeated, long-term microwave energy exposure 18). Therefore, it is of interest to find alternative antifungal methods for removing C. albicans from dentures. After water, tea (Camellia sinensis) is the second most popular drink worldwide and has recently received the attention of the pharmaceutical and scientific communities due to the plethora of associated natural therapeutic compounds 19,20). Tea is generally harmless and ...

show abstract

Evaluating the efficiency of humic acid to remove micro‐organisms from denture base material

Abstract: Humic acid could be used as an alternative 'natural' solution for denture-cleaning agent.

Cited by 4 publications

References 37 publications

Clinical study of the biofilm of implant‐supported complete dentures in healthy patients

Clinical study of the biofilm of implant‐supported complete dentures in healthy patients

Evaluating the Efficiency of Humic Acid to Remove Stain form Artificial Teeth

Antifungal effect of tea extracts on <i>Candida albicans </i>

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