Evaluating the gene expression level of IL-12 in the fibrous layer of hepatic hydatid cysts isolated from slaughtered animals

Faridnia, Roghiyeh; Tolouei, Sepideh; Khanahmad-Shahreza, Hosein; Kalani, Hamed; Yousefi-Darani, H

doi:10.1007/s00580-014-2059-7

Cited by 5 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With their work, Amri et al, suggested that protoscoleces’ excretory/secretory components allegedly play a crucial role in the stimulation of the immune system by leading to IL-12 production. Our results are not in agreement with these observations from human or bovine infections as CE-infected animals did not show significant levels of IFN-γ and IL-12 [ 28 , 50 ]. Nonetheless, our findings are similar to the study described by Fardinia et al [ 51 ], which did not observe differences in IL-12 expression between normal and infected sheep.…”

Section: Discussioncontrasting

confidence: 99%

Evaluation of the Local Immune Response to Hydatid Cysts in Sheep Liver

Biase

Prisco

Pepe

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

In order to characterize the inflammatory phenotype of livers of sheep naturally infected by cystic echinococcosis, 100 sheep livers have been macroscopically assessed for the presence of hydatid cysts and sampled for histopathological and molecular analysis. According to gross and microscopic examination, livers were subsequently classified into three groups: normal liver (Group A), liver with the presence of fertile hydatid cysts (Group B), and liver with the presence of sterile hydatid cysts (Group C). Immunohistochemical analyses were accomplished using primary antibodies anti-Iba1, anti-CD3, anti-CD20, anti-TGF-β, and anti-MMP9. Finally, real-time PCR was performed in order to estimate the concentration levels of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleukin (IL)-12, IL-10, and TGF-β. Immunohistochemical analysis showed a diffuse immunolabelling of mononuclear cells for Iba-1 and TGF-β and a higher amount of CD20+ B cells compared to CD3+ T cells in both Groups B and C. The expression levels of Th-1-like immune cytokines TNF-α, INF-γ, and IL-12 did not show significant statistical differences. However, we found a significant increase in expression levels of Th-2 immune cytokines TGF-β and IL-10 in Groups B and C compared to Group A. Taken together, our findings suggest that macrophages have a predominant role in the local immune response to cystic echinococcosis. Moreover, we can speculate that Th2 immunity may be dominant, corroborating the idea that B cells are decisively essential in the control of the immune response during parasitic infection and that the immunomodulatory role of IL-10 and TGF-β may ensure the persistence of the parasite within the host.

show abstract

Section: Discussioncontrasting

confidence: 99%

Evaluation of the Local Immune Response to Hydatid Cysts in Sheep Liver

Biase

Prisco

Pepe

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

“…So probably this liquid can be a curative material for cancer treatment. Regulation of the immune system by hydatid cyst [26,27] may be considered as a mechanism for anti-cancer effect of this parasite. In this context it has been shown that cytokine secretion and dendritic cell differentiation is modulated by the hydatid cyst fluid of Echinococcus granulosus [28].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Hydatid Cyst Liquid on Melanoma Tumor Growth in Mouse Model

Darani

Sharafi

Mokarian

et al. 2016

BJMMR

View full text Add to dashboard Cite

Introduction: Hydatid cyst is the larval stage of Echinococcus granulosus which develops in various human and livestock animals tissues. This hydatid cyst composed of several layers. Previous investigations have shown that the prevalence of hydatid cyst in cancer patients is significantly lower than in healthy individuals. In animal models preventive effect of the hydatid cyst antigens in reduction of tumor growth has been shown. Due to the effect of live protosculex and Original Research Articlehydatid cyst antigens on reducing the growth of cancer cells in culture medium, in this study therapeutic effect of hydatid cyst liquid on the growth of melanoma tumor in mice (C57 black) has been investigated. Methods: In this Experimental study, the population studies were C57 black mice. Six groups of mice were injected with 10 6 melanoma cells in their chest site subcutaneously. Then the mice were injected with hydatid cyst fluid either with or without albendazole. From the second week, the tumor size was measured using a calipers and tumor area was calculated and the mean tumor area compared with those of control groups using One-way ANOVA test. Results: The mean tumor area, in groups which injected with hydatid cyst fluid (case groups), was less than the mean tumor area in the control groups and this difference was statistically significant. Conclusion:This study showed that hydatid cyst fluid may have therapeutic effect on melanoma cancer growth.

show abstract