Evaluating the potential for resistance development in Gram-negative bacteria to antimicrobial blue light (at 405 nm): in vitro and in vivo studies

Leanse, Leon G.; Harrington, Olivia D.; Fang, Yanyan; Ahmed, Imran; Goh, Xueping Sharon

doi:10.1117/12.2506910

Cited by 6 publications

(7 citation statements)

References 16 publications

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“…A common hypothesis is that aBL can photoexcite endogenous photosensitizers (e.g., iron-free porphyrins) in bacterial cells and subsequently leads to the production of reactive oxidative species (ROS) (18)(19)21). We previously reported that aBL is capable of inactivating a range of gram-positive and gram-negative bacteria (18,22,23), including multidrug-resistant strains (24,25).…”

Section: Introductionmentioning

confidence: 99%

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Liu

Chang

Ferrer‐Espada

et al. 2020

Photochem & Photobiology

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Moraxella catarrhalis is one of the major otopathogens of otitis media (OM) in childhood. M. catarrhalis tends to form biofilm, which contributes to the chronicity and recurrence of infections, as well as resistance to antibiotic treatment. In this study, we aimed to investigate the effectiveness of antimicrobial blue light (aBL; 405 nm), an innovative nonpharmacological approach, for the inactivation of M. catarrhalis OM. M. catarrhalis either in planktonic suspensions or 24‐h old biofilms were exposed to aBL at the irradiance of 60 mW cm−2. Under an aBL exposure of 216 J cm−2, a >4‐log10 colony‐forming units (CFU) reduction in planktonic suspensions and a >3‐log10 CFU reduction in biofilms were observed. Both transmission electron microscopy and scanning electron microscopy revealed aBL‐induced morphological damage in M. catarrhalis. Ultraperformance liquid chromatography results indicated that protoporphyrin IX and coproporphyrin were the two most abundant species of endogenous photosensitizing porphyrins. No statistically significant reduction in the viability of HaCaT cells was observed after an aBL exposure of up to 216 J cm−2. Collectively, our results suggest that aBL is potentially an effective and safe alternative therapy for OM caused by M. catarrhalis. Further in vivo studies are warranted before this optical approach can be moved to the clinics.

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Section: Introductionmentioning

confidence: 99%

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Liu

Chang

Ferrer‐Espada

et al. 2020

Photochem & Photobiology

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“…Similar to aPDI , aBL inactivation of microorganisms is thought to be a multi‐target damaging process . As a consequence, the likelihood for the development of aBL‐resistance by microorganisms is less than that of antibiotic resistance .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Blue Light Inactivation of Microbial Isolates in Biofilms

et al. 2019

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Background and Objectives Biofilms cause more than 80% of infections in humans, including more than 90% of all chronic wound infections and are extremely resistant to antimicrobials and the immune system. The situation is exacerbated by the fast spreading of antimicrobial resistance, which has become one of the biggest threats to current public health. There is consequently a critical need for the development of alternative therapeutics. Antimicrobial blue light (aBL) is a light‐based approach that exhibits intrinsic antimicrobial effect without the involvement of exogenous photosensitizers. In this study, we investigated the antimicrobial effect of this non‐antibiotic approach against biofilms formed by microbial isolates of multidrug‐resistant bacteria. Study Design/Materials and Methods Microbial isolates of Acinetobacter baumannii, Candida albicans, Escherichia coli, Enterococcus faecalis, MRSA, Neisseria gonorrhoeae, Pseudomonas aeruginosa, and Proteus mirabilis were studied. Biofilms were grown in microtiter plates for 24 or 48 hours or in the CDC biofilm reactor for 48 hours and exposed to aBL at 405 nm (60 mW/cm2, 60 or 30 minutes). The anti‐biofilm activity of aBL was measured by viable counts. Results The biofilms of A. baumannii, N. gonorrhoeae, and P. aeruginosa were the most susceptible to aBL with between 4 and 8 log10 inactivation after 108 J/cm2 (60 mW/cm2, 30 minutes) or 216 J/cm2 (60 mW/cm2, 60 minutes) aBL were delivered in the microplates. On the contrary, the biofilms of C. albicans, E. coli, E. faecalis, and P. mirabilis were the least susceptible to aBL inactivation (−0.30, −0.24, −0.84, and −0.68 log10 inactivation, respectively). The same aBL treatment in biofilms developed in the CDC biofilm reactor, caused −1.68 log10 inactivation in A. baumannii and −1.74 and −1.65 log10 inactivation in two different strains of P. aeruginosa. Conclusions aBL exhibits potential against pathogenic microorganisms and could help with the significant need for new antimicrobials in clinical practice to manage multidrug‐resistant infections. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

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“…The accepted mechanism of aBL inactivation is through photoexcitation of endogenous porphyrins that result in the generation of reactive oxygen species and apoptosis [5]. It is an especially attractive antimicrobial strategy, because previous studies have demonstrated that resistance development to aBL by bacteria through serial exposure is highly unlikely [6,7]. However, the antimicrobial efficacy of aBL is dependent on the infecting agent, with certain bacterial species being comparatively more tolerant of aBL-mediated killing than others [8].…”

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confidence: 99%

Quinine Enhances Photo-Inactivation of Gram-Negative Bacteria

Leanse

Dong

Goh

et al. 2019

The Journal of Infectious Diseases

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Background Antimicrobial resistance is a significant concern to public health, and there is a pressing need to develop novel antimicrobial therapeutic modalities. Methods In this study, we investigated the capacity for quinine hydrochloride (Q-HCL) to enhance the antimicrobial effects of antimicrobial blue light ([aBL] 405 nm wavelength) against multidrug-resistant (MDR) Gram-negative bacteria in vitro and in vivo. Results Our findings demonstrated the significant improvement in the inactivation of MDR Pseudomonas aeruginosa and Acinetobacter baumannii (planktonic cells and biofilms) when aBL was illuminated during Q-HCL exposure. Furthermore, the addition of Q-HCL significantly potentiated the antimicrobial effects of aBL in a mouse skin abrasion infection model. In addition, combined exposure of aBL and Q-HCL did not result in any significant apoptosis when exposed to uninfected mouse skin. Conclusions In conclusion, aBL in combination with Q-HCL may offer a novel approach for the treatment of infections caused by MDR bacteria.

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Evaluating the potential for resistance development in Gram-negative bacteria to antimicrobial blue light (at 405 nm): in vitro and in vivo studies

Cited by 6 publications

References 16 publications

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Antimicrobial Blue Light Inactivation of Microbial Isolates in Biofilms

Quinine Enhances Photo-Inactivation of Gram-Negative Bacteria

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