Evaluating the potential of immobilized bacterial consortium for black liquor biodegradation

Abstract

Two indigenous bacterial strains, Bacillus megaterium ETLB-1 (accession no. KC767548) and Pseudomonas plecoglossicida ETLB-3 (accession no. KC767547), isolated from soil contaminated with paper mill effluent, were co-immobilized on corncob cubes to investigate their biodegradation potential against black liquor (BL). Results exhibit conspicuous reduction in color and lignin of BL upto 913.46 Co-Pt and 531.45 mg l(-1), respectively. Reduction in chlorophenols up to 12 mg l(-1) was recorded with highest release of chloride ions, i.e., 1290 mg l(-1). Maximum enzyme activity for lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (LAC) was recorded as 5.06, 8.13, and 8.23 U ml(-1), respectively, during the treatment. Scanning electron microscopy (SEM) revealed successful immobilization of bacterial strains in porous structures of biomaterial. Gas chromatography/mass spectroscopy (GC/MS) showed formation of certain low molecular weight metabolites such as 4-hydroxy-benzoic acid, 3-hydroxy-4-methoxybenzaldehyde, ferulic acid, and t-cinnamic acid and removal of majority of the compounds (such as teratogenic phthalate derivatives) during the period of treatment. Results demonstrated that the indigenous bacterial consortium possesses excellent decolorization and lignin degradation capability which enables its commercial utilization in effluents treatment system.

Analysis of the composition of culturable airborne microorganisms in the archaeological excavation protection site of the Nanhai No. 1 Ancient Shipwreck

“…1 has some parts contaminated by fungi, and the long-term biodegradation of destructive fungi can lead to the destruction of the wooden structure of the hull. Some bacteria such as Bacillus and Pseudomonas ( Paliwal et al, 2015 ; Takeuchi et al, 2017 ) can also degrade cellulose and lignin and destroy the hull structure. Therefore, bacteria were added as a new indicator in the 2019–2020 period.…”

Black Liquor Valorization by Using Marine Protist Thraustochytrium striatum and the Preliminary Metabolic Mechanism Study

“…Black liquor has primarily been treated by the thermochemical process for fuel production and alkali recovery, such as concentration and incineration, gasification, , and hydrothermal liquefaction. , In addition to these thermochemical strategies, detoxification of black liquor through biological treatment has attracted increasing research interest. White rot fungi have been successfully applied due to their powerful extracellular ligninolytic enzyme system, , bacteria were also studied for their compelling environmental adaptability and biochemical versatility. , The organic matter in black liquor can be a potential carbon source for the biosynthesis of microbial products. For instance, sugars separated from black liquor have been used for biobutanol production. , …”

“…White rot fungi have been successfully applied due to their powerful extracellular ligninolytic enzyme system, 7,8 bacteria were also studied for their compelling environmental adaptability and biochemical versatility. 9,10 The organic matter in black liquor can be a potential carbon source for the biosynthesis of microbial products. For instance, sugars separated from black liquor have been used for biobutanol production.…”

Performance characteristics of single-stage biohythane production by immobilized anaerobic bacteria

“…Other than the fixed bed, there are many methods that could be applied to improve the bacterial growth such as cell immobilization. This immobilized-cell system has shown many reactor performance advantages over suspended culture such as help avoiding cell-washout [10], has easy product recovery [11], could enlarge cell density [12] and give cost effective since the cells could be used several times without losing significant activities [13]. Moreover, the polymer matrix gel can protect the cells from harsh conditions and prevent them from nutrient competition and overgrowth [14].…”