Evaluating the potential of marine Bacteriovorax sp. DA5 as a biocontrol agent against vibriosis in Litopenaeus vannamei larvae

Wen, Chongqing; Xue, Mei; Liang, Hang; Zhou, Shining

doi:10.1016/j.vetmic.2014.07.022

Cited by 28 publications

(21 citation statements)

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“…The fact that the experimental results concerned native predators in NWs adds relevance to the findings. Other investigators using different experimental models have also reported a reduction of prey bacteria by Halobacteriovorax and the potential of the predators to affect the mortality of susceptible bacterial populations in aquatic systems (Richards et al, 2012;Li et al, 2014;Wen et al, 2014). Richards et al (2012) used several strains of V. parahaemolyticus and a strain of V. vulnificus as amendments to a natural seawater sample and observed a decline in their numbers along with an increase in Halobacteriovorax as we observed in the experiments described in this report.…”

Section: Discussionsupporting

confidence: 78%

“…The findings of this and other studies demonstrating the impact of BALO predation in aquatic systems (Li et al, 2011;Richards et al, 2012;Wen et al, 2014) argues for recognition of their potential role as biological agents of bacterial mortality along with viruses and protists. Johnke et al (2014) have made a strong case for assessing the potential of multiple micro-predators rather than focusing on a single predator, as many previous studies have done.…”

Section: Discussionmentioning

confidence: 63%

“…To establish the microcosms, the filtered water samples were amended with a high concentration (10 8 ml -1 ) of V. parahaemolyticus prey cells to efficiently elicit and amplify the predators' responses. Others have used this same approach in Halobacteriovorax predation experiments (Richards et al, 2012;Wen et al, 2014) and achieved the same pattern of reduction of the prey and increase in predator numbers. Some studies using viral microcosms have also involved high numbers of total bacteria (up to 3 × 10 7 ml -1 ) (Guixa-Boixereu et al, 1999).…”

Section: Discussionmentioning

confidence: 91%

“…The fact that Halobacteriovorax are also abundant in the surface microlayer (Williams, 1987) suggests that they prey on the large numbers of bacteria present. Although high concentrations of prey were used in this study, it should be noted that BALOs are typically capable of reducing prey concentrations via population dynamics similar to those shown in Figures 2 and 3 at prey concentrations as low as of 1 × 10 4 ml -1 , albeit over a longer time period (Ahmed, 2007;Richards et al, 2012;Li et al, 2014;Wen et al, 2014).…”

Section: Discussionmentioning

confidence: 97%

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Halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality

et al. 2015

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Predation on bacteria and accompanying mortality are important mechanisms in controlling bacterial populations and recycling of nutrients through the microbial loop. The agents most investigated and seen as responsible for bacterial mortality are viruses and protists. However, a body of evidence suggests that predatory bacteria such as the Halobacteriovorax (formerly Bacteriovorax), a Bdellovibrio-like organism, contribute substantially to bacterial death. Until now, conclusive evidence has been lacking. The goal of this study was to better understand the contributors to bacterial mortality by addressing the poorly understood role of Halobacteriovorax and how their role compares with that of viruses. The results revealed that when a concentrated suspension of Vibrio parahaemolyticus was added into microcosms of estuarine waters, the native Halobacteriovorax were the predators that responded first and most rapidly. Their numbers increased by four orders of magnitude, whereas V. parahaemolyticus prey numbers decreased by three orders of magnitude. In contrast, the extant virus population showed little increase and produced little change in the prey density. An independent experiment with stable isotope probing confirmed that Halobacteriovorax were the predators primarily responsible for the mortality of the V. parahaemolyticus. The results show that Halobacteriovorax have the potential to be significant contributors to bacterial mortality, and in such cases, predation by Halobacteriovorax may be an important mechanism of nutrient recycling. These conclusions add another dimension to bacterial mortality and the recycling of nutrients.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 97%

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Halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality

et al. 2015

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“…Halobacteriovorax has been used to reduce mortalities from vibrios in shrimp larvae (18) and from Aeromonas veronii in fish (19). Richards et al (20) determined that the low levels of Halobacteriovorax naturally present in seawater were capable of significantly reducing Vibrio parahaemolyticus and Vibrio vulnificus levels in both seawater and oysters.…”

mentioning

confidence: 99%

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Richards

Fay

Uknalis

et al. 2016

Appl Environ Microbiol

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c Halobacteriovorax (formerly Bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. Four strains of Halobacteriovorax originally isolated in Vibrio parahaemolyticus O3:K6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. This technique was essential, since 0.45-m filtration alone was unable to remove infectious Vibrio minicells, as determined by scanning electron microscopy and cultural methods. Purified Halobacteriovorax strains were screened for predation against other V. parahaemolyticus strains and against Vibrio vulnificus, Vibrio alginolyticus, Escherichia coli O157:H7, and Salmonella enterica serovar Typhimurium DT104, all potential threats to seafood safety. They showed high host specificity and were predatory only against strains of V. parahaemolyticus. In addition, strains of Halobacteriovorax that were predatory for E. coli O157:H7 and S. Typhimurium DT104 were isolated from a tidal river at 5 ppt salinity. In a modified plaque assay agar, they killed their respective prey over a broad range of salinities (5 to 30 ppt). Plaques became smaller as the salinity levels rose, suggesting that the lower salinities were optimal for the predators' replication. These species also showed broader host specificity, infectious against each other's original hosts as well as against V. parahaemolyticus strains. In summary, this study characterized strains of Halobacteriovorax which may be considered for use in the development of broad-based biocontrol technologies to enhance the safety of commercially marketed shellfish and other foods. Bdellovibrio and like organisms (BALO) are Gram-negative, aerobic bacteria which are predatory toward other Gram-negative bacteria and consist of freshwater/terrestrial and marine forms. Bdellovibrio and like organisms are divided into four genera, Bdellovibrio, Bacteriolyticum, Peredibacter, and Bacteriovorax, which was recently renamed Halobacteriovorax (1). These organisms enter into a susceptible host bacterium and reside within the periplasmic space where they utilize the cytoplasmic nutrients of the host to support growth and replication. The replicative form, known as a bdelloplast, elongates within the host and septates into progeny cells as the host is lysed, releasing cells capable of attacking more prey. Halobacteriovorax organisms are the marine forms, which are small, polar flagellated, highly motile, intracellular predators in the class Deltaproteobacteria family Halobacteriovoraceae (1).Bdellovibrio and like organisms, particularly the nonhalophilic Bdellovibrio bacteriovorus, have been proposed for use in reducing the levels of phytopathogens associated with bacterial blights in soybeans and rice (2, 3) and reducing the levels of Salmonella enterica serovar Typhimurium on freshwater fish fillets (4). Other uses involve the treatment of various infections in animals, including bovine keratoconjunctivitis caused by Mora...

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Antibacterial Activities of Bdellovibrio and like Organisms in Aquaculture

Najnine

Cao

Cai

2020

The Ecology of Predation at the Microscale

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Evaluating the potential of marine Bacteriovorax sp. DA5 as a biocontrol agent against vibriosis in Litopenaeus vannamei larvae

Cited by 28 publications

References 28 publications

Halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality

Halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality

Purification and Host Specificity of Predatory Halobacteriovorax Isolates from Seawater

Antibacterial Activities of Bdellovibrio and like Organisms in Aquaculture

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