Evaluating the Relationship Between Serum Level of Interleukin-6 and Rheumatoid Arthritis Severity and Disease Activity

Rajaei, Elham; Mowla, Karim; Hayati, Qodratollah; Ghorbani, Ali; Dargahi-Malamir, Mehrdad; Hesam, Saeed; Zayeri, Zeinab Deris

doi:10.2174/1573397115666190206144223

Cited by 12 publications

(11 citation statements)

References 20 publications

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“…As expected, in comparison with parameters for RA disease activity, VEGF and IL-6 levels were correlated with TJC, SJC, ESR, CRP, and DAS28 (Fig. 3b), consistent with earlier reports [16][17][18]34,35 . In addition, as shown in the heat map and correlation plots in Fig.…”

Section: Resultssupporting

confidence: 92%

Angiogenic cytokines can reflect the synovitis severity and treatment response to biologics in rheumatoid arthritis

et al. 2020

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Angiogenesis and synoviocyte hyperplasia, called 'pannus,' are pathologic hallmarks of rheumatoid arthritis (RA). To determine the clinical significance of angiogenic cytokines in RA, the levels of pro-angiogenic cytokines, including VEGF, placenta growth factor (PlGF), and IL-6, were measured in the synovial fluid (SF, n = 54) and sera of RA patients (n = 157) using ELISA. Patients (n = 103) with disease activity score 28 (DAS28) > 3.2, which indicates moderate to high RA activity, underwent follow-up blood sampling at 6 months after treatment with conventional disease-modifying anti-rheumatic drugs (c-DMARD) or biologic DMARD (b-DMARD) including an anti-TNFα antibody, an anti-IL-6 antibody, and abatacept. Ultrasonography (US) was performed on affected joints to define the synovitis severity at the time of sampling. Consequently, in the SF of RA patients, PlGF and IL-6 levels correlated well with synovitis severity determined by US. In RA sera, VEGF and IL-6 levels were elevated in proportion to synovitis severity, correlating with conventional markers for disease activity, including ESR, CRP, and DAS28. In c-DMARD users (n = 53), serially monitored levels of serum VEGF, IL-6, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) all decreased in good and moderate responders but not in nonresponders. In b-DMARD users (n = 49), only serum VEGF well represented the treatment response, while CRP nonspecifically decreased irrespective of the treatment outcome. By multivariable analysis, serum ΔVEGF, but not ΔESR or ΔCRP, was an independent factor associated with good and moderate responses to DMARD. In summary, the angiogenic cytokines PlGF and VEGF represent the synovitis severity of RA assessed by US. In patients receiving b-DMARD, serum VEGF may be more valuable than CRP in reflecting the treatment response.

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Section: Resultssupporting

confidence: 92%

Angiogenic cytokines can reflect the synovitis severity and treatment response to biologics in rheumatoid arthritis

et al. 2020

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“…As IL-6 is necessary for the induction of IL-17A production by neutrophils [28, 29], we measured the serum levels of IL-16, which were increased in RA patients (7.99 ± 16.7 pg/ml in RA vs 0.67 ± 1.18 pg/ml in HC). Additionally, there was a positive correlation between IL-6 serum levels and DAS28-CRP ( p < 0.0001, r = 0.39), as others have observed [30–32], but IL-6 levels did not correlate with an increased numbers of IL-17A+ neutrophils (data not shown).…”

Section: Resultssupporting

confidence: 75%

Endogenous stimulation is responsible for the high frequency of IL-17A-producing neutrophils in patients with rheumatoid arthritis

González-Orozco

Barbosa-Cobos

Santana-Sanchez

et al. 2019

Allergy Asthma Clin Immunol

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Background: Neutrophils play an important role in the pathogenesis of rheumatoid arthritis (RA). It has recently been reported that in addition to T helper (Th) 17 cells, other cells, including neutrophils, produce IL-17A, an important inflammatory cytokine involved in the pathogenesis of RA. The purpose of this study was to examine the presence of interleukin 17A-producing neutrophils in patients with RA. Methods: We performed a cross-sectional study including 106 patients with RA and 56 healthy individuals. Whole peripheral blood cells were analyzed by flow cytometry to identify CD66b+ CD177+ IL-17A+ neutrophils and CD3+ CD4+ IL-17A+ T cells. Serum levels of IL-17A and IL-6 were measured by means of cytometry bead array (CBA). In purified neutrophils, mRNA levels of IL-17 and RORγ were measured by RT-PCR. In addition, purified neutrophils from patients and healthy controls were stimulated with the cytokines IL-6 and IL-23 to evaluate differences in their capacity to produce IL-17A. Results: Neutrophils from RA patients expressed IL-17 and RORγ mRNA. Consequently, these cells also expressed IL-17A. Serum IL-17A levels but not Th17 cell numbers were increased in RA patients. Neutrophils positive for cytoplasmic IL-17A were more abundant in patients with RA (mean 1.2 ± 3.18%) than in healthy individuals (mean 0.07 ± 0.1%) (p < 0.0001). Although increased IL-17A+ neutrophil numbers were present in RA patients regardless of disease activity (mean 6.5 ± 5.14%), they were more frequent in patients with a more recent diagnosis (mean time after disease onset 3.5 ± 4.24 years). IL-6 and IL-23 induced the expression of RORγ but failed to induce IL-17A expression by neutrophils from RA patients and healthy individuals after a 3 h stimulation. Conclusion: IL-17A-producing neutrophils are increased in some RA patients, which are not related to disease activity but have an increased frequency in patients with recent-onset disease. This finding suggests that IL-17Aproducing neutrophils play an early role in the development of RA.

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“…It is also associated with increased susceptibility of diseases such as diabetes mellitus and rheumatoid arthritis. [ 41 42 ] Leptin plays a dual role as a hormone and as a cytokine, affecting endocrine functions, bone metabolism, energy homeostasis, and of inflammatory responses. [ 43 ] Therefore, it appears that the interaction of obesity and periodontitis increases the levels of some IM to a greater extent than when these conditions act separately, suggesting that obese patients with periodontitis may be at an even increased risk for systemic complication related to systemic inflammatory burden.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers in biological fluids in adults with periodontitis and/or obesity: A meta-analysis

Brum

Duarte

Canto

et al. 2020

J Indian Soc Periodontol

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Evaluating the Relationship Between Serum Level of Interleukin-6 and Rheumatoid Arthritis Severity and Disease Activity

Cited by 12 publications

References 20 publications

Angiogenic cytokines can reflect the synovitis severity and treatment response to biologics in rheumatoid arthritis

Angiogenic cytokines can reflect the synovitis severity and treatment response to biologics in rheumatoid arthritis

Endogenous stimulation is responsible for the high frequency of IL-17A-producing neutrophils in patients with rheumatoid arthritis

Biomarkers in biological fluids in adults with periodontitis and/or obesity: A meta-analysis

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