Evaluating the relationship of autumn buttercup (<i>Ranunculus acriformis</i> var. <i>aestivalis</i>) to some close congeners using random amplified polymorphic DNA

Buren, Renée Van; Harper, Kimball T.; Andersen, William R.; Stanton, David J.; Seyoum, Seifu; England, J. L.

doi:10.1002/j.1537-2197.1994.tb15476.x

Cited by 37 publications

(2 citation statements)

References 27 publications

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“…The most widely used methodology of molecular systematics involves PCR amplification and comparative alignment of orthologous DNA sequences (Kimura 198 1 ;Taberlet et al 199 l), but other PCR-based marker techniques have also become popular. Thus, the amplification of anonymous fragments from genomic DNA using single arbitrary primers (random amplified polymorphic DNA (RAPD) analysis; Williams et al 1990) is increasingly applied for evaluating relationships at the species level and below (e.g., Adams and Demeke 1993;Williams and St. Clair 1993;Van Buren et al 1994;Stammers et al 1995;Ramser et al 1996). In a related technique, (un)anchored microsatellite-complementary primers are used to amplify inter-repeat regions (Gupta et al 1994;Zietkiewicz et al 1994;Weising et al 1995a).…”

Section: Introductionmentioning

confidence: 99%

Molecular marker based taxonomy and phylogeny of Guinea yam (Dioscorea rotundata–D.cayenensis)

Ramser¹,

Weising²,

Terauchi³

et al. 1997

Genome

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Four different molecular techniques were used to assess relationships among 21 accessions of Guinea yam (Dioscorea rotundata and Dioscorea cayenensis) and 21 accessions belonging to seven putative progenitor species. Random amplified polymorphic DNA (RAPD) and microsatellite-primed PCR (MP-PCR) analysis yielded 246 informative characters that were transformed into a matrix of pairwise distances and analyzed by neighbor joining or split decomposition. Both methods gave congruent results. Well-separated groups were formed that corresponded to their species designation. Dioscorea rotundata and D. cayenensis accessions were clearly separated from each other, supporting the concept that both are distinct species. Two morphological intermediates grouped together with D. rotundata. All investigated species fell into two main clusters, one comprising D. rotundata, D. cayenensis, Dioscorea abyssinica, Dioscorea liebrechtsiana, and Dioscorea praehensilis, the other comprising Dioscorea smilacifolia, Dioscorea minutiflora, Dioscorea burkilliana, and Dioscorea togoensis. The same grouping was also obtained by comparative sequence analysis of chloroplast DNA, which supports earlier studies of nuclear rDNA variation and chloroplast restriction fragment length polymorphisms. We also analyzed the same set of Dioscorea samples with the recently developed random amplified microsatellite polymorphism (RAMPO) technique. A series of diagnostic RAMPO bands was identified that clearly distinguished between D. rotundata and D. cayenensis. Some of these bands could also be traced back to the putative progenitors of both species. The evolutionary origin of Guinea yam is discussed in light of the present results.

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Section: Introductionmentioning

confidence: 99%

Molecular marker based taxonomy and phylogeny of Guinea yam (Dioscorea rotundata–D.cayenensis)

Ramser¹,

Weising²,

Terauchi³

et al. 1997

Genome

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“…(Wolff & Peters-van Rijn, 1993), to reveal taxonomic relationships within e.g. Ranunculus taxa (Van Buren et al, 1994), for characterization of varieties in Thobrama (cocoa) clones (Wilde, Waugh & Powell, 1992) and in Brassica oleraceu L. (Kresovich et al, 1992). It has also been used to devise conservation strategies in the rare and endangered Orevillea scapigera (Rossetto, Weaver & Dixon, 1995) and in the island endemic Malacothamnus fasciculatus var.…”

Section: Introductionmentioning

confidence: 99%

RAPD and morphological analysis of the rare plant species Vicia pisiformis (Fabaceae)

Black-Samuelsson

Eriksson

Gustafsson

et al. 1997

Biological Journal of the Linnean Society

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The amount and pattern of genetic variation was surveyed in two Swedish and three Czech populations of the rare perennial forest plant &pisifotmi~. This species has a mainly easterlycontinental European distribution and has few and small populations in Sweden. It is classified as 'vulnerable' on the Swedish Red Data lit. Seeds from natural populations were collected and grown under controlled conditions in growth chambers. The variation was estimated in growth and fecundity traits and with Random Amplified Polymorphic DNA, RAPD. Low inter-and intra-population variation in R4PD-markers was found using 11 primers, with a similarity index (Wetton) for the families of 0.98. In contrast, multivariate analysis of variance showed significant morphological differences withim and between populations. Also in the univariate ANOVAs, a number of the traits showed si@cant betweenand/or within population differentiation. Cluster analysis for the morphological traits and RAPDs (UPGMA) did not structure the variation of families in accordance with their geographical distance. A Mantel test based on comparisons between Mahalonobis and Jaccard distance for morphological and RAPD data, respectively, did not reveal any significant correlation between the two matrices. It is concluded that if a genetic conservation program is to be applied on Vicia pi$mzi, different sampling strategies are needed to capture morphological vs RAPD variation. This is, to our knowledge, the first investigation that compares RAPD and morphological variation in a threatened plant species. 0 1997 The Linnean Society of London ADDITIONAL

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Genetic diversity of the narrow endemicAllium aaseae(Alliaceae)

Smith

Pham

1996

American J of Botany

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Low levels of genetic variability are common for a number of geographically restricted plants: these data are consistent with theoretical expectations that small populations should be genetically depauperate. However, in some species, high levels of variability have been found in rare species. Allium aaseae is a rare, narrow endemic in the foothills of the Boise Front of southwestern Idaho. Genetic variation in the rare endemic A. aaseae, and nearby populations of the more common species, A. simillimum, was examined with randomly amplified polymorphic DNA (RAPD) data. Eight populations of A. aaseae and six populations of A. simillimum (three near the range of A. aaseae, and three distant populations) of 25 individuals each were examined for this analysis. Genetic diversity as determined with RAPD markers of both species examined in this study is largely found within and not among populations. Levels of genetic diversity are high, especially for a narrow endemic species such as A. aaseae. Proportion of RAPD loci polymorphic was high in both species, although slightly higher in the more common A. simillimum. Because these higher levels of genetic variability run counter to theory, alternative explanations beyond population size must be invoked to explain the levels of genetic diversity found in this study. Possible explanations are (1) A. aaseae is only recently derived from A. simillimum, (2) hybridization between A. aaseae and A. simillimum is occurring, (3) multiple origins of A. aaseae, (4) populations of A. simillimum included in the analysis are all A. aaseae, (5) A. aaseae and A. simillimum are conspecific, and (6) an artifact of RAPD data.

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Evaluating the relationship of autumn buttercup (Ranunculus acriformis var. aestivalis) to some close congeners using random amplified polymorphic DNA

Cited by 37 publications

References 27 publications

Molecular marker based taxonomy and phylogeny of Guinea yam (Dioscorea rotundata–D.cayenensis)

Molecular marker based taxonomy and phylogeny of Guinea yam (Dioscorea rotundata–D.cayenensis)

RAPD and morphological analysis of the rare plant species Vicia pisiformis (Fabaceae)

Genetic diversity of the narrow endemicAllium aaseae(Alliaceae)

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