Evaluating the role of astrocytes on β-estradiol effect on seizures of Pilocarpine epileptic model

Sarfi, Masoumeh; Salmani, Mahmoud Elahdadi; Goudarzi, Iran; Boluki, Taghi Lashkar; Abrari, Kataneh

doi:10.1016/j.ejphar.2017.01.005

Cited by 13 publications

(9 citation statements)

References 66 publications

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“…The regulation of GLT-1 by estradiol is very well established in non-neuronal brain cells 37 , 77 , 78 . Interestingly, it has been also shown that stimulation with β-estradiol results in an increased hippocampal GLT-1 expression 79 . These observations are in agreement with the improved MWM performance as well as with the enhancement of LTP and with the observed reduced protein levels of GLT-1 found here specifically in FLRT2 +/− females.…”

Section: Discussionmentioning

confidence: 98%

Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice

Cicvaric

Yang

Bulat

et al. 2018

Sci Rep

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The Fibronectin Leucine-Rich Transmembrane protein 2 (FLRT2) has been implicated in several hormone -and sex-dependent physiological and pathological processes (including chondrogenesis, menarche and breast cancer); is known to regulate developmental synapses formation, and is expressed in the hippocampus, a brain structure central for learning and memory. However, the role of FLRT2 in the adult hippocampus and its relevance in sex-dependent brain functions remains unknown. We here used adult single-allele FLRT2 knockout (FLRT2+/−) mice and behavioral, electrophysiological, and molecular/biological assays to examine the effects of FLRT2 haplodeficiency on synaptic plasticity and hippocampus-dependent learning and memory. Female and male FLRT2+/− mice presented morphological features (including body masses, brain shapes/weights, and brain macroscopic cytoarchitectonic organization), indistinguishable from their wild type counterparts. However, in vivo examinations unveiled enhanced hippocampus-dependent spatial memory recall in female FLRT2+/− animals, concomitant with augmented hippocampal synaptic plasticity and decreased levels of the glutamate transporter EAAT2 and beta estrogen receptors. In contrast, male FLRT2+/− animals exhibited deficient memory recall and decreased alpha estrogen receptor levels. These observations propose that FLRT2 can regulate memory functions in the adulthood in a sex-specific manner and might thus contribute to further research on the mechanisms linking sexual dimorphism and cognition.

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Section: Discussionmentioning

confidence: 98%

Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice

Cicvaric

Yang

Bulat

et al. 2018

Sci Rep

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“…In contrast, blockade of NMDA receptors with antagonists attenuates the impact of estrogens on neural cells that correlate of memory, such as long-term potentiation 319 . It is noteworthy that estrogens act on both neural cells and astrocytes to mediate neuroprotective effects in the hippocampus of estradiol-treated female rats 320 . Specifically, estradiol treatment improves density of neural cells, upregulates glutamine synthetase activity, and increases astrocyte glutamine transporter expression in the hippocampus.…”

Section: Effects Of Estrogens On Cns Neurtransmissionmentioning

confidence: 99%

“…Specifically, estradiol treatment improves density of neural cells, upregulates glutamine synthetase activity, and increases astrocyte glutamine transporter expression in the hippocampus. Therefore, neuroprotective properties of estrogens are reasonably linked to astrocytic activity 320 .…”

Section: Effects Of Estrogens On Cns Neurtransmissionmentioning

confidence: 99%

Effects of Estrogens on Central Nervous System Neurotransmission: Implications for Sex Differences in Mental Disorders

Krolick

Zhu

Shi

2018

Progress in Molecular Biology and Translational Science

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Nearly one of every five US individuals aged twelve years old or older live with certain types of mental disorders. Men are more likely to use various types of substances, while women tend to be more susceptible to mood disorders, addiction, and eating disorders, all of which are risks associated with suicidal attempts. Fundamental sex differences exist in multiple aspects of functions and activities of neurotransmitter-mediated neural circuits in the central nervous system (CNS). Dysregulation of these neural circuits would lead to various types of mental disorders. The potential mechanisms of sex differences in the CNS neural circuitry regulating mood, reward, and motivation are only beginning to be understood, although they have been largely attributed to the effects of sex hormones on CNS neurotransmission pathways. Understanding this topic is important for developing prevention and treatment of mental disorders that should be tailored differently between men and women. Studies using animal models have provided important insights into pathogenesis, mechanisms, and new therapeutic approaches of human diseases, but some concerns remain to be addressed. The purpose of this chapter is to integrate human and animal studies involving the effects of sex hormone estrogens on CNS neurotransmission, reward processing, and associated mental disorders. We provide an overview of existing evidence for the physiological, behavioral, cellular and molecular actions of estrogens in the context of controlling neurotransmission in the CNS circuits regulating mood, reward and motivation, and discuss related pathology that leads to related mental disorders.

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“…Some studies demonstrated similar changes in EAAT2 expression in different animal models of epilepsy [25,26,27]. However, other studies did not confirm these findings [28,29].…”

Section: Introductionmentioning

confidence: 99%

Ceftriaxone Treatment Affects EAAT2 Expression and Glutamatergic Neurotransmission and Exerts a Weak Anticonvulsant Effect in Young Rats

Zaitsev

Malkin

Postnikova

et al. 2019

IJMS

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Epilepsy is a common neurological disorder. Despite the availability of a wide range of antiepileptic drugs, these are unsuccessful in preventing seizures in 20–30% of patients. Therefore, new pharmacological strategies are urgently required to control seizures. Modulation of glutamate uptake may have potential in the treatment of pharmacoresistant forms of epilepsy. Previous research showed that the antibiotic ceftriaxone (CTX) increased the expression and functional activity of excitatory amino acid transporter 2 (EAAT2) and exerted considerable anticonvulsant effects. However, other studies did not confirm a significant anticonvulsant effect of CTX administration. We investigated the impacts of CTX treatment on EAAT expression and glutamatergic neurotransmission, as well its anticonvulsant action, in young male Wistar rats. As shown by a quantitative real-time polymerase chain reaction (qPCR) assay and a Western blot analysis, the mRNA but not the protein level of EAAT2 increased in the hippocampus following CTX treatment. Repetitive CTX administration had only a mild anticonvulsant effect on pentylenetetrazol (PTZ)-induced convulsions in a maximal electroshock threshold test (MEST). CTX treatment did not affect the glutamatergic neurotransmission, including synaptic efficacy, short-term facilitation, or the summation of excitatory postsynaptic potentials (EPSPs) in the hippocampus and temporal cortex. However, it decreased the field EPSP (fEPSP) amplitudes evoked by intense electrical stimulation. In conclusion, in young rats, CTX treatment did not induce overexpression of EAAT2, therefore exerting only a weak antiseizure effect. Our data provide new insight into the effects of modulation of EAAT2 expression on brain functioning.

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Evaluating the role of astrocytes on β-estradiol effect on seizures of Pilocarpine epileptic model

Cited by 13 publications

References 66 publications

Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice

Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice

Effects of Estrogens on Central Nervous System Neurotransmission: Implications for Sex Differences in Mental Disorders

Ceftriaxone Treatment Affects EAAT2 Expression and Glutamatergic Neurotransmission and Exerts a Weak Anticonvulsant Effect in Young Rats

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