2023
DOI: 10.3390/genes14050994
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Evaluating the Usefulness of Human DNA Quantification to Predict DNA Profiling Success of Historical Bone Samples

Abstract: This study assessed the usefulness of DNA quantification to predict the success of historical samples when analyzing SNPs, mtDNA, and STR targets. Thirty burials from six historical contexts were utilized, ranging in age from 80 to 800 years postmortem. Samples underwent library preparation and hybridization capture with two bait panels (FORCE and mitogenome), and STR typing (autosomal STR and Y-STR). All 30 samples generated small (~80 bp) autosomal DNA target qPCR results, despite mean mappable fragments ran… Show more

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Cited by 5 publications
(9 citation statements)
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“…Replicate amplifications were carried out for extracts B-7Ac, B-7A1, and B-28Ac due to suspected inhibition based on previous research ( Table S2 ). 13 Burials 3 and 4 failed to generate reportable profiles (<4 loci), as shown in Table S5 . Burial 3 had the lowest quantification result of the four DNA extracts that generated a value for the Y-DNA target ( Table 2 ); therefore, a lower recovery was expected.…”
Section: Resultsmentioning
confidence: 99%
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“…Replicate amplifications were carried out for extracts B-7Ac, B-7A1, and B-28Ac due to suspected inhibition based on previous research ( Table S2 ). 13 Burials 3 and 4 failed to generate reportable profiles (<4 loci), as shown in Table S5 . Burial 3 had the lowest quantification result of the four DNA extracts that generated a value for the Y-DNA target ( Table 2 ); therefore, a lower recovery was expected.…”
Section: Resultsmentioning
confidence: 99%
“…A reduced total dsDNA input was used for extract B-7Ac due to signs of inhibition observed in previous research. 13 At adapter ligation the stock concentration (15 μM) of UDI adapter was used for all samples and RBs. NCs and PCs received a 1.5 μM dilution due to a dsDNA input ≤1ng.…”
Section: Methodsmentioning
confidence: 99%
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“…While the use of microarrays has long been the state of the art for SNP genotyping, the requirement for high amounts of pure and intact DNA limits its application for several types of challenging samples [10,18]. The recent progress in sequencing techniques has enabled the analysis of low quantities of degraded DNA [19][20][21][22] and the successful generation of genotypes from aged samples [1, 10,14,[23][24][25][26][27]. The possibility of re-analyzing difficult DNA samples with more sophisticated methods has also spurred the continuation and reopening of unsolved investigations of "cold cases" and unidentified human remains [14,24,26].…”
Section: Introductionmentioning
confidence: 99%