Preventing man-in-the-middle (MiTM) attack using Artificial Neural Network refers to an in depth analysis of how calls are made vis-a-viz the structure of the inter-related operations that binds the respective subsystems within the GSM Architecture during calls. Calls in the GSM network is a request from aMobile Station (MS). This request has faced severe attacks due to the network’s access to Internet presence that has made its way into cellular telephony, creating a vulnerable and susceptible network attack such as Man-in-the-middle. This paper proffer solution to Man-in-the-middle attack during GSM calls by using Artificial Neural Network which can be embedded into the Protocol Stack to detect network intrusion and prevent Man-in-the-middle attack to obtain hitch-free local and international calls.