Evaluation and comparison of biocontrol and conventional fungicides for control of postharvest potato tuber diseases

Gachango, Esther; Kirk, W. W.; Schafer, Robert L.; Wharton, P. S.

doi:10.1016/j.biocontrol.2012.07.005

Cited by 44 publications

(26 citation statements)

References 19 publications

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“…The algaecide and azoxystrobin were the only two active ingredients where mycelial growth of both Phytophthora species occurred at all concentrations. This was a surprising result for azoxystrobin as this active ingredient has been shown to be effective against Phytophthora species in several studies (Gachango, Kirk, Schafer, & Wharton, 2012;Matheron & Porchas, 2000;Rebollar-Alviter et al, 2007). Qi et al (2012) also found that in vitro mycelial growth of Phytophthora capsici was less sensitive to the strobilurins, azoxystrobin and trifloxystrobin than to other fungicides, such as manipropamid, dimethomorph and flumorph.…”

Section: Discussionmentioning

confidence: 99%

Chemical control of two Phytophthora species infecting the canopy of Monterey pine (Pinus radiata)

et al. 2017

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Twelve active ingredients were screened for their ability to control foliage disease caused by Phytophthora kernoviae and Phytophthora pluvialis. Inhibition of mycelial growth and sporangial production of both pathogens were assessed in in vitro assays after exposure to three concentrations of each active ingredient. While most of these active ingredients inhibited mycelial growth in vitro, due to their widespread use, phosphite, copper oxychloride and metalaxyl-M were selected for further study. Four rates of each active ingredient were applied to two Pinus radiata genotypes, and detached needle assays at 6 and 90 days post-treatment were used to determine treatment efficacy and persistence. Untreated needles showed significantly larger lesions than all fungicide-treated needles after exposure to P. pluvialis or P. kernoviae on both sampling dates. Efficacy and persistence of the three active ingredients did not increase with increase in concentration. Larger lesions were formed on more susceptible genotype after inoculation with both Phytophthora species, even when higher rates of active ingredients were applied. Phosphite, copper oxychloride and metalaxyl-M have potential to protect commercially planted P. radiata from these two Phytophthora species.|

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Section: Discussionmentioning

confidence: 99%

Chemical control of two Phytophthora species infecting the canopy of Monterey pine (Pinus radiata)

et al. 2017

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“…GOD antifungal activity could be attributed to the presence of hydrogen peroxide generated by the action of the GOD. Gachango et al [35] and Miller et al [36] indicated that hydrogen peroxide moderately reduced the development of tuber late blight, pink rot and Pythium leak.…”

Section: Discussionmentioning

confidence: 99%

Purification, biochemical characterization and antifungal activity of a novel Aspergillus tubingensis glucose oxidase steady on broad range of pH and temperatures

Kriaa

Hammami

Sahnoun

et al. 2015

Bioprocess Biosyst Eng

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This study was carried out to evaluate the in vitro and in vivo antifungal efficiency of Aspergillus tubingensis CTM 507 glucose oxidase (GOD) against plant pathogenic fungi. GOD displayed a wide inhibitory spectrum toward different fungi at a concentration of 20 AU. The GOD had a strong inhibitor effect on mycelia growth and spore germination of Pythium ultimum. Interestingly, the GOD exhibited a potent in vivo antifungal effect against P. ultimum responsible for potato plants disease. The antifungal GOD was purified 13-fold with 27 % yield and a specific activity of 3435 U/mg. The relative molecular mass of the GOD was 180 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The GOD activity was optimum at pH 4.5 and 60 °C. It was found to be stable over a large pH range (3-9). It also displayed a marked thermostability with a 50-min half-life at 65 °C. The 10 residues of the N-terminal sequence of the purified GOD (S-K-G-S-A-V-T-T-P-D) showed no homology to the other reported GOD, identifying a novel GOD. FTIR spectroscopic analysis revealed the presence of C-O and C=O groups corresponding to a D-glucono-lactone. The findings indicated that GOD is the first A. tubingensis-produced fungicide ever reported to exhibit such promising biological properties. It could become a natural alternative to synthetic fungicides to control certain important plant microbial diseases.

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“…For Fusarium species that cause dry rot of potato tubers or Phytophthora infestans that causes late blight in potato plants, there are no known biocontrol agents as efficacious as synthetic chemical pesticides (Stephan et al, 2005;Gachango et al, 2012). Furthermore, there is little information on interactions between such potato pathogens and other microbes encountered in the field (or on the stored tuber) which may have potential as biocontrol agents (Schisler et al, 1995;Slininger et al, 2007;Clermont et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol agents promote growth of potato pathogens, depending on environmental conditions

Cray

Connor

Stevenson

et al. 2016

Microbial Biotechnology

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SummaryThere is a pressing need to understand and optimize biological control so as to avoid over‐reliance on the synthetic chemical pesticides that can damage environmental and human health. This study focused on interactions between a novel biocontrol‐strain, Bacillus sp. JC12GB43, and potato‐pathogenic Phytophthora and Fusarium species. In assays carried out in vitro and on the potato tuber, the bacterium was capable of near‐complete inhibition of pathogens. This Bacillus was sufficiently xerotolerant (water activity limit for growth = 0.928) to out‐perform Phytophthora infestans (~0.960) and challenge Fusarium coeruleum (~0.847) and Fusarium sambucinum (~0.860) towards the lower limits of their growth windows. Under some conditions, however, strain JC12GB43 stimulated proliferation of the pathogens: for instance, Fusarium coeruleum growth‐rate was increased under chaotropic conditions in vitro (132 mM urea) by >100% and on tubers (2‐M glycerol) by up to 570%. Culture‐based assays involving macromolecule‐stabilizing (kosmotropic) compatible solutes provided proof‐of‐principle that the Bacillus may provide kosmotropic metabolites to the plant pathogen under conditions that destabilize macromolecular systems of the fungal cell. Whilst unprecedented, this finding is consistent with earlier reports that fungi can utilize metabolites derived from bacterial cells. Unless the antimicrobial activities of candidate biocontrol strains are assayed over a full range of field‐relevant parameters, biocontrol agents may promote plant pathogen infections and thereby reduce crop yields. These findings indicate that biocontrol activity, therefore, ought to be regarded as a mode‐of‐behaviour (dependent on prevailing conditions) rather than an inherent property of a bacterial strain.

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Evaluation and comparison of biocontrol and conventional fungicides for control of postharvest potato tuber diseases

Cited by 44 publications

References 19 publications

Chemical control of two Phytophthora species infecting the canopy of Monterey pine (Pinus radiata)

Chemical control of two Phytophthora species infecting the canopy of Monterey pine (Pinus radiata)

Purification, biochemical characterization and antifungal activity of a novel Aspergillus tubingensis glucose oxidase steady on broad range of pH and temperatures

Biocontrol agents promote growth of potato pathogens, depending on environmental conditions

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