Evaluation and comparison of efficacy of three different storage media, coconut water, propolis, and oral rehydration solution, in maintaining the viability of periodontal ligament cells

Abstract

Background:Two of the most critical factors affecting the prognosis of an avulsed tooth after replantation are extra oral dry time and the storage medium in which the tooth is placed before treatment is rendered. However, the ability of a storage/transport medium to support cell viability can be more important than the extra oral time to prevent ankylosis and replacement resorption.Aim:Purpose of this study was evaluation and comparison of efficacy of a new storage medium, oral rehydration solution (ORS) with coconut water, and propolis in maintaining the viability of periodontal ligament (PDL) cells by using a collagenase-dispase assay.Materials and Methods:40 teeth were selected with intact crown which were advised for Orthodontic extraction having healthy PDL. Teeth were then randomly divided into three experimental storage solution groups. Other 10 were divided into positive and negative control groups (5 each).Statistical Analysis and Result:The results were statistically analyzed with analysis of variance and multiple range by using post hoc tests. The results of the prevailing study indicated that coconut water group demonstrated a significantly higher number of viable PDL cells than propolis 50%, and ORS. There was no significant difference between coconut water and propolis 50% groups.

Coconut milk and probiotic milk as storage media to maintain periodontal ligament cell viability: an in vitro study

“…In the current study, trypan blue dye exclusion technique for determination of cell viability was used as it is quick and easy to perform (5,8). In the current study, trypan blue dye exclusion technique for determination of cell viability was used as it is quick and easy to perform (5,8).…”

“…However, most commonly, studies (5,(8)(9)(10)(11) involving measurements of PDL cell activity have concentrated on measuring cell viability as an indicator of cell survival due to its simplicity and ease of implementation. However, most commonly, studies (5,(8)(9)(10)(11) involving measurements of PDL cell activity have concentrated on measuring cell viability as an indicator of cell survival due to its simplicity and ease of implementation.…”

Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

“…However, the osmolality values did not vary with the addition of neutralizing agents. Most studies evaluating CW as a storage medium for avulsed teeth did not investigate the pH neutralization 18,24 . To date, only three studies have been concerned with adjusting the pH to physiological conditions 11,14,15 .…”

“…However, these studies have not compared the effects of other neutralizer agents, potentially applicable for this purpose, in order to better preserve the CW properties. In spite of the influence of pH and the industrialization process on CW characteristics, its ability to replace fluids and nutrients justifies the great interest in this solution in recent years 11,17,18,24 .…”

“…Despite the favorable characteristics of CW, studies involving this solution as a storage medium have shown contradictory results. Some studies report that CW showed better performance than other storage media such as HBSS or milk in terms of maintaining PDL cell viability after avulsion [11][12][13]15,18 ; another study addressed low capability to preserve cells 14 . The pH adjustment of this solution seems to be an indispensable stage for its production at an industrial scale 9 .…”