Evaluation and comparison of population genetics software in Rabari Tribe of Gujarat population

Abstract

Background Today, when forensic experts talk about quantifiable hereditary traits, they do not just depend on the assessment and examination of DNA profiles but also relate them to the population structures. The use of high-throughput molecular marker technologies and advanced statistical and software tools have improved the accuracy of human genetic diversity analysis in many populations with limited time and resources. The present study aimed to investigate the genomic diversity in Gujarat’s Rabari population, using 20 autosomal genetic markers. Numerous bio-statistical software programs are available for the interpretation of population data in forensics. These statistics deal with the measurement of uncertainty and also provides a probability of a random match. The present paper aims to provide a practical guide to the analysis of population genetics data. Three statistical software packages named Cervus, Genepop, and Fstat are compared and contrasted. The comparison is performed on the profiles obtained from fifty unrelated blood samples of healthy male individuals. DNA was extracted using the organic extraction method, 20 autosomal STR loci were amplified using PowerPlex 21 kit (Promega, Madison, WI, USA) and detected on 3100 Genetic Analyser (Life Technologies Corporation, Carlsbad, CA, USA). Results A total of 170 alleles were observed in the Rabari Tribe of Gujarat population, and allele frequencies ranged from 0.010 to 0.480. The highest allele frequency detected was 0.480 for allele 9 at locus TH01. Based on heterozygosity and the polymorphism information content, FGA may be considered as the most informative markers. Both the combined power of discrimination (CPD) and the combined power of exclusion (CPE) for the 20 analyzed loci were higher than 0.999999. The combined match probability (CPM) for all 20 loci was 2.5 × 10−22. Conclusions With respect to the results, the 20 STR loci are highly polymorphic and discriminating in the Gujarat population and could be used for forensic practice and population genetics studies. However, Fstat demonstrated better genetic software for analysis of the demographic structure of a specific or set of populations.

Effect of Optimism, Happiness and Self-esteem on Quality of Life among Yemeni University Students

“…These results are similar to the results of the study of Abdul-Khalek (2017), which found that happiness and self-esteem predict the quality of life. It is also similar to the results of Mishra's (2014) study, which found that optimism predicted quality of life. The quality of life is affected by a person's view on life in general and his vision of the future in particular.…”

Allele frequencies of 15 Autosomal STR loci in Some of Iraqi population

“…Allelic frequency data obtained from the unrelated individual in a population is essential. It is the key to obtain reliable results in an analysis of DNA profiles [12]. Specificity of Short tandem repeat (STR) genetic locus is highly polymorphic repeat sequences.…”