Evaluation and Comparison of the Antibacterial Activity againstStreptococcus mutansof Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: Anin vitroStudy

Abstract

IntroductionStreptococcus mutans has been implicated as primary microorganisms which cause dental caries in humans. There has been an increased interest in the therapeutic properties of some medicinal plants and natural compounds which have demonstrated antibacterial activities. Grape is one of the plants of this group which contains tannin and polyphenolic compound.AimTo evaluate and compare antibacterial activity of grape seed extract at different concentrations with chlorhexidine gluconate against S. mutans.Materials and methodsGrape seeds were extracted with ethanol/water ratio of 70:30 volume/volume. The extracts were filtered through Whatman No. 1 filter paper until it becomes colorless. Streptococcus mutans strains were taken. To check the antimicrobial properties of grape seed extract at different concentration and chlorhexidine gluconate, they were added to S. mutans strain and incubated for 48 hours than colony-forming units/mL were checked.ResultsGrape seed extract at higher concentration were found to be more potent against S. mutans. Chlorhexidine gluconate was found to have most potent antibacterial action compared to all different concentrations of grape seed extract.ConclusionGrape seed extract as a natural antimicrobial compound has inhibitory effect against S. mutans.How to cite this articleSwadas M, Dave B, Vyas SM, Shah N. Evaluation and Comparison of the Antibacterial Activity against Streptococcus mutans of Grape Seed Extract at Different Concentrations with Chlorhexidine Gluconate: An in vitro Study. Int J Clin Pediatr Dent 2016;9(3):181-185.

Prevention of dental caries by grape seed extract supplementation: A systematic review

“…Particularly in the case of S. mutans, studies reviewed give support to the idea that dental GSE treatment may inhibit biofilm formation and bacterial colonization in the dental environment (Furiga et al, 2009(Furiga et al, , 2014Swadas et al, 2016;Thimothe et al, 2007;Zhao et al, 2014). Of the five studies cited, three found significant inhibition of S. mutans biofilm formation in single strain (Singla et al, 2018;Zhao et al, 2014) and multiorganism (Furiga et al, 2014) conditions by GSE.…”

Efecto antimicrobiano de extractos acuosos de la cáscara, pulpa y semilla de uva (vitis vinifera) sobre streptococcus mutans, estudio in vitro

“…En los países latinoamericanos pertenecientes al área andina como el Ecuador, se presentan una variedad de plantas, cuya estructura cuenta con diferentes aceites esenciales y polifenoles (19) que incitan a buscar a partir de extractos vegetales y fuentes naturales de fácil acceso, a elementos antioxidantes con propiedades biológicas y antibacterianas (16) , sin efectos colaterales, que consigan actuar sobre cepas de microrganismos responsables de diferentes enfermedades (20) .…”

“…La uva se muestra como un fruto de uso frecuente yfácil obtención, sin embargo cuando se trata de obtener extractos a partir de ella, nos encontramos ante una gran variedad de técnicas (19) , lo cual dificulta la obtención de un resultado conclusivo (18) , la técnica de obtención de extracto empleada en este estudio si bien es aceptada, desencadena resultados que merecen ser probados ejecutando otras técnicas de obtención (20) , sin embargo, los resultados demostraron que los extractos de semilla al 100% cuentan con una actividad elevada que podría explicarse por la capacidad de antioxidantes relacionada al contenido de polifenoles totales (21) .…”

Comparison of Efficacy of Diode Laser and Grape Seeds Extract as Cavity Disinfectants in Primary Teeth- an in vitro study

“…[14,15] Grape Seed extract (GSE) is one of the natural products that was found to be rich in polyphenolic compounds such as epicatechin and proanthocyanidins which are known for their antibacterial effect. [16] This study was, thus, conducted to compare the antibacterial effects of diode laser and GSE to CHX and investigate the effects of using them as cavity disinfectants on bond strength of glass ionomer restorations to dentine in primary teeth. In the present work, the null hypotheses tested were (1) no difference existed in antibacterial activity against Streptococcus mutans (S.mutans) between diode laser, GSE and CHX and (2) the tested cavity disinfectants had no effect on shear bond strength of glass ionomer to dentin in primary teeth.…”