Evaluation and Interlaboratory Validation of a Selective Agar for Phosphatidylinositol-Specific Phospholipase C Activity Using a Chromogenic Substrate To Detect Listeria monocytogenes from Foods

Jinneman, Karen C.; Hunt, Jan M.; Eklund, Cheryl A.; Wernberg, Jane S.; Sado, Patricia N.; Johnson, Janelle M.; Richter, Richelle S.; Torres, Selene T.; Ayotte, Eugene D.; Eliasberg, Stacey J.; Istafanos, Phillip; Bass, Deborah; Kexel-Calabresa, Nancy; Lin, Wen; Barton, Curtis N.

doi:10.4315/0362-028x-66.3.441

Cited by 13 publications

(9 citation statements)

References 10 publications

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“…by revealing esculinase (b-glucosidase) activity, a metabolic enzyme common to all Listeria species, but do not distinguish L. monocytogenes from other species (Leclercq, 2004). Typical colonies are selected from these selective differential agars and submitted to further biochemical tests and hemolytic activity to determine the identity of the species (Jinneman et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Performance of a chromogenic medium for the isolation of Listeria monocytogenes in food

et al. 2008

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Section: Introductionmentioning

confidence: 99%

“…The genus Listeria is composed by six species, with Listeria monocytogenes being the only significant human pathogen (Jinneman et al, 2003). L. monocytogenes is responsible for sporadic cases and outbreaks of listeriosis associated with a variety of foods, including meat products, raw vegetables and dairy products (Leclercq, 2004).…”

Section: Introductionmentioning

confidence: 99%

Performance of a chromogenic medium for the isolation of Listeria monocytogenes in food

et al. 2008

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“…Previous studies have demonstrated that ALOA compares favourably with Oxford agar for the recovery of L. monocytogenes (Vlaemynck et al. 2000; Jinneman et al. 2003; Sacchetti et al.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a new chromogenic agar for the detection of Listeria in food

Willis¹,

Baalham²,

Greenwood³

et al. 2006

J Appl Microbiol

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Aim: Rapid identification of Listeria in food is important in protecting consumers from infection. The development of chromogenic media such as agar Listeria according to Ottaviani and Agosti (ALOA) has allowed more rapid detection of Listeria monocytogenes, with presumptive identification of this pathogenic species after only 24 h of incubation. The aim of this study was to evaluate Oxoid chromogenic Listeria agar (OCLA) in comparison with ALOA and a traditional, nonchromogenic medium, Oxford agar. Methods and Results: Media were compared using pure cultures, spiked food samples and naturally contaminated samples. Whilst development of typical colony morphology took 48 h on Oxford agar, Listeria spp. were frequently detected after 24 h of incubation on OCLA and ALOA. There was no significant difference in recovery between the two chromogenic media. Conclusions: Results indicate that OCLA gives equivalent recovery of Listeria spp. compared with ALOA. Whilst L. monocytogenes was frequently detected after 24 h of incubation, a 48‐h incubation time was necessary to ensure detection of both L. monocytogenes and other Listeria spp. Significance and Impact of the Study: This study has shown that a commercially available chromogenic medium other than ALOA is appropriate for use in the international standard method. The commercial availability of more than one medium will facilitate the more widespread use of the method, thus increasing confidence in the ability to detect L. monocytogenes in food in the presence of other Listeria spp.

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“…Evaluations of chromogenic media for the isolation of L. monocytogenes have been reported previously (Jinneman et al. 2003; Sacchetti et al.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of chromogenic media for the detection of Listeria species in food

Greenwood¹,

Willis²,

Doswell³

et al. 2005

J Appl Microbiol

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Aims: The aim of this study was to evaluate the performance of chromogenic agars, Agar Listeria according to Ottaviani and Agosti (ALOA) and Rapid L. mono agar, compared with Oxford agar for the enumeration and detection of Listeria species in food. Methods and Results: A total of 170 food samples were examined using the three plating media. Listeria species were isolated from 63 samples. In contrast to Oxford agar, detection of Listeria colonies on chromogenic media was as good after 24 h of incubation of plates as after 48 h. While there was no significant difference in recovery of Listeria monocytogenes on the three media, recovery of other Listeria species was significantly poorer on Rapid L. mono agar compared with Oxford and ALOA agars. Recovery of species other than L. monocytogenes was significantly improved by including a secondary enrichment stage in the detection method. Conclusions: Using chromogenic agars, presumptive identification of L. monocytogenes is possible after 24 h, compared with 3-4 days using Oxford agar. However, the poor detection of species other than L. monocytogenes on Rapid L. mono agar is a disadvantage of this medium. Significance and Impact of the Study: This study provides new information regarding the isolation of Listeria species other than L. monocytogenes from food using chromogenic plating media. This is important, as nonpathogenic Listeria species act as markers for the likelihood of presence of L. monocytogenes and allow preventive action to be taken to avoid its presence.

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Evaluation and Interlaboratory Validation of a Selective Agar for Phosphatidylinositol-Specific Phospholipase C Activity Using a Chromogenic Substrate To Detect Listeria monocytogenes from Foods

Cited by 13 publications

References 10 publications

Performance of a chromogenic medium for the isolation of Listeria monocytogenes in food

Performance of a chromogenic medium for the isolation of Listeria monocytogenes in food

Evaluation of a new chromogenic agar for the detection of Listeria in food

Evaluation of chromogenic media for the detection of Listeria species in food

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