Evaluation and performance characteristics of the automated coagulation analyzer ACL TOP

Appert-Flory, Anny; Fischer, Florence; Jambou, Didier; Toulon, Pierre

doi:10.1016/j.thromres.2006.12.002

Cited by 30 publications

(25 citation statements)

References 15 publications

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“…Concerning within-and between-run precision, the obtained CVs were within the criteria for acceptance in most evaluated assays, and results are similar to already published evaluations of other fully-automated coagulation analyzers with similar characteristics, such as the STAR (Flanders et al, 2002), and CA 7000 (Fischer et al, 2006;Dorn-Beineke et al, 2005), as well as of the ACL TOP (Appert-Flory et al, 2007;Eschwè ge, Catillon & Robert, 2006).…”

Section: Discussionsupporting

confidence: 86%

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Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Miloš

Herak

Kuric

et al. 2009

Int J Lab Hematology

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ACL TOP is a fully automated coagulation analyzer, designed for simultaneous measurement of routine and special coagulation parameters. We evaluated analytical and technical performance characteristics of the coagulation system composed of the ACL TOP analyzer and HemosIL reagent group for the determination of routine clotting (PT, APTT, fibrinogen, FVII, and FVIII), chromogenic (protein C) and immunological assays (FXIII antigen). Within run and between run CVs ranged from 0.9% to 7.7% and from 2.0% to 14.8% respectively. The obtained CVs for imprecision of calibration curves were <5% of PT and <7% for fibrinogen. The method comparison study showed good correlation between results obtained on the ACL TOP and BCS/BCT analyzers, with correlation co-efficients ranging from 0.709 to 0.955, but with significantly different results for PT INR, APTT, fibrinogen and protein C, and wide dispersion of differences observed in difference plots for most assays. Despite good correlation and agreement for FVIII, problems in measuring FVIII<10% were encountered. The effective througput for the ACL TOP and BCS was 151 and 212 PT/APTT/fibrinogen tests per hour, respectively. Although the ACL TOP is designed to run multiple assays on a large number of samples, software limitations make the instrument suitable rather for mid-sized laboratories.

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Section: Discussionsupporting

confidence: 86%

“…, 2006; Dorn‐Beineke et al. , 2005), as well as of the ACL TOP (Appert‐Flory et al. , 2007; Eschwège, Catillon & Robert, 2006).…”

Section: Discussionmentioning

confidence: 99%

Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Miloš

Herak

Kuric

et al. 2009

Int J Lab Hematology

View full text Add to dashboard Cite

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“…Test results were expressed in percentage (%) where 100% was equal to 1.00 IU mL )1 . It can be mentioned that the ACL TOP analyser has the software capabilities to run factor assays at various dilutions simultaneously with an on-screen presentation of factor parallelism testing [9,10]. However, that option was not used in this study.…”

Section: Reagents and Assay Proceduresmentioning

confidence: 99%

Measurement of factor VIII activity using one‐stage clotting assay: a calibration curve has not to be systematically included in each run

Lattes¹,

Appert-Flory²,

Fischer³

et al. 2010

Haemophilia

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Coagulation factor VIII (FVIII) is usually evaluated using activated partial thromboplastin time-based one-stage clotting assays. Guidelines for clotting factor assays indicate that a calibration curve should be included each time the assay is performed. Therefore, FVIII measurement is expensive, reagent- and time-consuming. The aim of this study was to compare FVIII activities obtained using the same fully automated assay that was calibrated once (stored calibration curve) or each time the assay was performed. Unique lots of reagents were used throughout the study. We analysed 255 frozen plasma samples from patients who were prescribed FVIII measurement including treated and untreated haemophilia A patients. Twenty-six runs were performed on a 28-week period, each including four lyophilized control and at most 10 patient plasma samples. In control samples, FVIII activities were not significantly different when the assay was performed using the stored calibration curve or was daily calibrated. The same applied to FVIII activities in patient plasma samples that were not significantly different throughout the measuring range of activities [68.3% (<1-179) vs. 67.6% (<1-177), P=0.48] and no relevant bias could be demonstrated when data were compared according to Bland and Altman. These results suggest that in the studied technical conditions, performing the FVIII assay using a stored calibration curve is reliable, for at least 6 months. Therefore, as far as the same lots of reagents are used, it is not mandatory to include a calibration curve each time the FVIII assay was performed. However, this strategy has to be validated if the assay is performed in different technical conditions.

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“…For WBC count, specimens were collected in peripheral blood sampling microtainer tube containing K 2 EDTA and analyzed using an automated blood analyzer (Coulter LH 750) within 4 h from collection. The laboratory tests for fibrinogen were performed on blood collected with sodium citrate on ACL TOP (Instrumentation Laboratory, Milan, Italy), according to the manufacturer's specifications and using HemosIL reagent system 17 (Instrumentation Laboratory). To determine hsCRP plasma levels, blood was collected in lithium-heparin tube and concentrations were measured using an immunoassay technique (Siemens Healthcare Diagnostics, Den Hague, the Netherlands) with a lower detection limit of 0.2 mg/L carried out in Dimension® Vista™ 1500 analyzer (Siemens Diagnostics) according to the manufacturer's instructions.…”

Section: Blood Collection and Laboratory Measurementsmentioning

confidence: 99%

Prediction of abdominal aortic aneurysm calcification by means of variation of high-sensitivity C-reactive protein

Garrafa

Giacomelli

Ravanelli

et al. 2016

JRSM Cardiovascular Disease

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ObjectiveAbdominal aortic aneurysms are a major cause of death in developed countries, and thrombus and calcification of the aneurysm have been linked to increased complications. This study was conducted in order to identify the biochemical marker associated to the presence of intraluminal thrombus or calcification progression of the aneurysm.DesignSeveral clinical laboratory parameters were measured in patients with abdominal aortic aneurysms, in particular those already demonstrated to be related to the pathology, such as lipoprotein (a), white blood cell count, fibrinogen and high-sensitivity C-reactive protein. Most of the patients were analysed for the presence of thrombus or aorta calcification using CT angiography.ResultsUnlike previous findings, we found no association between intraluminal thrombus formation and lipoprotein (a), but we evidenced that patients with lower grade of calcification tend to have higher plasma high-sensitivity C-reactive protein values compared with patients with a higher degree of calcification. Instead, no association was found with either white blood cell count or fibrinogen level.ConclusionsThis study suggests that high-sensitivity C-reactive protein is a useful biomarker to assess the evolution of calcification and could be used in triaging patients to identify those who should undergo a rapid imaging, thus allowing prompt initiation of treatment or rule-out suspicious patients from non-essential imaging repetition.

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Evaluation and performance characteristics of the automated coagulation analyzer ACL TOP

Cited by 30 publications

References 15 publications

Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer – HemosIL reagents

Measurement of factor VIII activity using one‐stage clotting assay: a calibration curve has not to be systematically included in each run

Prediction of abdominal aortic aneurysm calcification by means of variation of high-sensitivity C-reactive protein

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