Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction

Chen, Xianghui; Cui, Kai; Xiu, Jiancheng; Lin, Hua; Liu, Yi; Zhou, Biying; Liang, Feng; Zha, Daogang; Bin, Jianping; Liu, Yili

doi:10.1007/s10554-009-9474-x

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…After 30 min, 2,3,5-triphenyltetrazolium chloride (TTC) staining was undertaken, to check for necrosis. 16…”

Section: Ventricular Arrhythmia Modelmentioning

confidence: 99%

Stabilizing Microtubules Decreases Myocardial Ischaemia-Reperfusion Injury

Wang

et al. 2011

J Int Med Res

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Microtubules are important components of the cytoskeleton that forms the backbone of myocardial architecture, sustaining its form and size. This study investigated whether stabilizing microtubules with paclitaxel (0.1 or 1 μM) could decrease myocardial ischaemia- reperfusion injury, and reduce myocardial infarct size and the incidence of ischaemic ventricular arrhythmia. Isolated rat hearts were used, with arrhythmia induced by regional ischaemia and myocardial infarcts induced by ischaemia-reperfusion. In these ex vivo rat models, paclitaxel decreased myocardial ischaemia-reperfusion injury, significantly reducing the incidence and severity of ischaemic ventricular arrhythmia and significantly decreasing infarct size.

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“…After 30 min, 2,3,5-triphenyltetrazolium chloride (TTC) staining was undertaken, to check for necrosis. 16…”

Section: Ventricular Arrhythmia Modelmentioning

confidence: 99%

Stabilizing Microtubules Decreases Myocardial Ischaemia-Reperfusion Injury

Wang

et al. 2011

J Int Med Res

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“…Another method of in vivo measurements is echocardiography. A correlation has been reported between infarct size measured by in vivo echocardiography and ex vivo TTC staining in both mice and rat [8,9]. Unfortunately, the individual variations between the two methods have been considerable.…”

Section: Introductionmentioning

confidence: 99%

In vivo visualization and ex vivo quantification of experimental myocardial infarction by indocyanine green fluorescence imaging

Sonin¹,

Papayan²,

Почкаева

et al. 2016

Biomed. Opt. Express

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Abstract:The fluorophore indocyanine green accumulates in areas of ischemia-reperfusion injury due to an increase in vascular permeability and extravasation of the dye. The aim of the study was to validate an indocyanine green-based technique of in vivo visualization of myocardial infarction. A further aim was to quantify infarct size ex vivo and compare this technique with the standard triphenyltetrazolium chloride staining. Wistar rats were subjected to regional myocardial ischemia (30 minutes) followed by reperfusion (n = 7). Indocyanine green (0.25 mg/mL in 1 mL of normal saline) was infused intravenously for 10 minutes starting from the 25th minute of ischemia. Video registration in the near-infrared fluorescence was performed. Epicardial fluorescence of indocyanine green corresponded to the injured area after 30 minutes of reperfusion. Infarct size was similar when determined ex vivo using traditional triphenyltetrazolium chloride assay and indocyanine green fluorescent labeling. Intravital visualization of irreversible injury can be done directly by fluorescence on the surface of the heart. This technique may also be an alternative for ex vivo measurements of infarct size.

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“…TTC is an inexpensive, rapid, and reliable method for myocardial IS measurement and has been used as the sole quantitative method in several investigations involving IS assessment [13], [39], [55], [56]. Moreover, TTC has been extensively utilized as the gold standard technique to validate new imaging methods of infarct area quantification, such as cardiac magnetic resonance imaging [57]–[61], contrast echocardiography [62]–[66], and two-dimensional speckle tracking imaging [67]. Nevertheless, the use of biochemical techniques, such as a dosage of troponin-I, may also be reliable for estimating myocardial IS and could confirm and emphasize our results concerning the effects of OR antagonists in exercise-induced cardioprotection.…”

Section: Discussionmentioning

confidence: 99%

Delta Opioid Receptors: The Link between Exercise and Cardioprotection

et al. 2014

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This study investigated the role of opioid receptor (OR) subtypes as a mechanism by which endurance exercise promotes cardioprotection against myocardial ischemia-reperfusion (IR) injury. Wistar rats were randomly divided into one of seven experimental groups: 1) control; 2) exercise-trained; 3) exercise-trained plus a non-selective OR antagonist; 4) control sham; 5) exercise-trained plus a kappa OR antagonist; 6) exercise-trained plus a delta OR antagonist; and 7) exercise-trained plus a mu OR antagonist. The exercised animals underwent 4 consecutive days of treadmill training (60 min/day at ∼70% of maximal oxygen consumption). All groups except the sham group were exposed to an in vivo myocardial IR insult, and the myocardial infarct size (IS) was determined histologically. Myocardial capillary density, OR subtype expression, heat shock protein 72 (HSP72) expression, and antioxidant enzyme activity were measured in the hearts of both the exercised and control groups. Exercise training significantly reduced the myocardial IS by approximately 34%. Pharmacological blockade of the kappa or mu OR subtypes did not blunt exercise-induced cardioprotection against IR-mediated infarction, whereas treatment of animals with a non-selective OR antagonist or a delta OR antagonist abolished exercise-induced cardioprotection. Exercise training enhanced the activities of myocardial superoxide dismutase (SOD) and catalase but did not increase the left ventricular capillary density or the mRNA levels of HSP72, SOD, and catalase. In addition, exercise significantly reduced the protein expression of kappa and delta ORs in the heart by 44% and 37%, respectively. Together, these results indicate that ORs contribute to the cardioprotection conferred by endurance exercise, with the delta OR subtype playing a key role in this response.

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Evaluation and simplified measurement of infarct size by myocardial contrast echocardiography in a rat model of myocardial infarction

Cited by 10 publications

References 11 publications

Stabilizing Microtubules Decreases Myocardial Ischaemia-Reperfusion Injury

Stabilizing Microtubules Decreases Myocardial Ischaemia-Reperfusion Injury

In vivo visualization and ex vivo quantification of experimental myocardial infarction by indocyanine green fluorescence imaging

Delta Opioid Receptors: The Link between Exercise and Cardioprotection

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