Evaluation and validation of reference genes for qPCR analysis to study climate change-induced stresses in Sinularia cf. cruciata (Octocorallia: Alcyonidae)

Shimpi, Gaurav G.; Vargas, Sergio; Wörheide, Gert

doi:10.1016/j.jembe.2016.06.001

Cited by 5 publications

(3 citation statements)

References 79 publications

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“…The dramatic upregulation of EF1A, typically considered a housekeeping gene, in the low salinity population (Vermilion Bay) and low salinity sampling date (Sister Lake B) is not reflected in the laboratory exposure. In an evaluation of qPCR reference genes used in climate change stress studies, Shimpi et al (2016) found EF1A to be a highly variable housekeeping gene in the octocoral Sinularia cf. cruciata during low-pH and thermal stress (Shimpi, Vargas, & Wörheide, 2016).…”

Section: Contrasting Laboratory and Wild Expressionmentioning

confidence: 99%

“…In an evaluation of qPCR reference genes used in climate change stress studies, Shimpi et al (2016) found EF1A to be a highly variable housekeeping gene in the octocoral Sinularia cf. cruciata during low-pH and thermal stress (Shimpi, Vargas, & Wörheide, 2016). EF1A has also been shown to play a part in the production of proteins associated with the heat shock response (Buckley, Andrew, & George, 2006;Wares & Schiebelhut, 2016).…”

Section: Contrasting Laboratory and Wild Expressionmentioning

confidence: 99%

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Synergistic Effects of Temperature and Salinity on the Gene Expression and Physiology of Crassostrea virginica

Jones

Johnson

Kelly

2019

Integrative and Comparative Biology

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The eastern oyster, Crassostrea virginica, forms reefs that provide critical services to the surrounding ecosystem. These reefs are at risk from climate change, in part because altered rainfall patterns may amplify local fluctuations in salinity, impacting oyster recruitment, survival, and growth. As in other marine organisms, warming water temperatures might interact with these changes in salinity to synergistically influence oyster physiology. In this study, we used comparative transcriptomics, measurements of physiology, and a field assessment to investigate what phenotypic changes C. virginica uses to cope with combined temperature and salinity stress in the Gulf of Mexico. Oysters from a historically low salinity site (Sister Lake, LA) were exposed to fully crossed temperature (20°C and 30°C) and salinity (25, 15, and 7 PSU) treatments. Using comparative transcriptomics on oyster gill tissue, we identified a greater number of genes that were differentially expressed (DE) in response to low salinity at warmer temperatures. Functional enrichment analysis showed low overlap between genes DE in response to thermal stress compared with hypoosmotic stress and identified enrichment for gene ontologies associated with cell adhesion, transmembrane transport, and microtubule-based process. Experiments also showed that oysters changed their physiology at elevated temperatures and lowered salinity, with significantly increased respiration rates between 20°C and 30°C. However, despite the higher energetic demands, oysters did not increase their feeding rate. To investigate transcriptional differences between populations in situ, we collected gill tissue from three locations and two time points across the Louisiana Gulf coast and used quantitative PCR to measure the expression levels of seven target genes. We found an upregulation of genes that function in osmolyte transport, oxidative stress mediation, apoptosis, and protein synthesis at our low salinity site and sampling time point. In summary, oysters altered their phenotype more in response to low salinity at higher temperatures as evidenced by a higher number of DE genes during laboratory exposure, increased respiration (higher energetic demands), and in situ differential expression by season and location. These synergistic effects of hypoosmotic stress and increased temperature suggest that climate change will exacerbate the negative effects of low salinity exposure on eastern oysters.

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Section: Contrasting Laboratory and Wild Expressionmentioning

confidence: 99%

Section: Contrasting Laboratory and Wild Expressionmentioning

confidence: 99%

Synergistic Effects of Temperature and Salinity on the Gene Expression and Physiology of Crassostrea virginica

Jones

Johnson

Kelly

2019

Integrative and Comparative Biology

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“…Despite the observed higher tolerance of octocorals to climate change-induced stress, their physiological response to these stimuli remains poorly studied. Wiens et al (2000) and Shimpi et al (2016) observed heat-shock protein overexpression in thermally stressed Dendronephthya klunzingeri and Sinularia cf. cruciata colonies, respectively.…”

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confidence: 99%

Transcriptional response of the calcification and stress response toolkits in an octocoral under heat and pH stress

et al. 2021

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Up to one‐third of all described marine species inhabit coral reefs, but the future of these hyperdiverse ecosystems is insecure due to local and global threats, such as overfishing, eutrophication, ocean warming and acidification. Although these impacts are expected to have a net detrimental effect on reefs, it has been shown that some organisms such as octocorals may remain unaffected, or benefit from, anthropogenically induced environmental change, and may replace stony corals in future reefs. Despite their potential importance in future shallow‐water coastal environments, the molecular mechanisms leading to the resilience to anthropogenically induced stress observed in octocorals remain unknown. Here, we use manipulative experiments, proteomics and transcriptomics to show that the molecular toolkit used by Pinnigorgia flava, a common Indo‐Pacific gorgonian octocoral, to deposit its calcium carbonate skeleton is resilient to heat and seawater acidification stress. Sublethal heat stress triggered a stress response in P. flava but did not affect the expression of 27 transcripts encoding skeletal organic matrix (SOM) proteins. Exposure to seawater acidification did not cause a stress response but triggered the downregulation of many transcripts, including an osteonidogen homologue present in the SOM. The observed transcriptional decoupling of the skeletogenic and stress‐response toolkits provides insights into the mechanisms of resilience to anthropogenically driven environmental change observed in octocorals.

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Molecular Responses of Sponges to Climate Change

Aguilar-Camacho

McCormack

2017

Climate Change, Ocean Acidification and Sponges

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Evaluation and validation of reference genes for qPCR analysis to study climate change-induced stresses in Sinularia cf. cruciata (Octocorallia: Alcyonidae)

Cited by 5 publications

References 79 publications

Synergistic Effects of Temperature and Salinity on the Gene Expression and Physiology of Crassostrea virginica

Synergistic Effects of Temperature and Salinity on the Gene Expression and Physiology of Crassostrea virginica

Transcriptional response of the calcification and stress response toolkits in an octocoral under heat and pH stress

Molecular Responses of Sponges to Climate Change

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