Evaluation and Validation of Utility of BD Glucose Vacutainer for Glycosylated Hemoglobin Assay for Timely Therapeutic Management of Diabetes Mellitus

Abstract

Blood glucose and glycosylated haemoglobin are the two vital biochemical tests widely prescribed for the therapeutic management of diabetes and require different anticoagulants during specimen collection. If both tests can be performed using single vacutainer, it reduces the laboratory costs at a larger scale and facilitates timely intervention. The anticoagulants NaF/Na 2 EDTA and K2 EDTA are used conventionally for blood glucose and HbA1c estimations respectively for specimen collection. In the current study we have investigated whether NAF/Na 2 EDTA can be used for HbA1c assay without the need of separate vacutainer i.e.K2EDTA. A total of 125 subjects (25 non-diabetic and 100 diabetic) were enrolled for this study. Parallel samples were collected in NaF/Na 2 EDTA and K2 EDTA to compare HbA1c levels in both the anticoagulants. The stability of HbA1c was elucidated in both anticoagulants till 72hrs of collection. The HbA1c levels in both anticoagulants showed excellent correlation (R 2 : 0.998). Bland and Altman analysis revealed that the bias between methods is -0.22 to +0.22%. The changes in HbA1c levels were unaltered till 72 hrs of collection in NaF/Na 2 EDTA and K2EDTA (mean SD: 0.066 vs. 0.058; %CV: 0.94 vs. 0.81). These results conclude that the same vacutainer used for glucose estimation can be used even for HbA1c till 72 hr of collection thus preventing the need of second sampling specifically in monitoring diabetic control. This study emphasized that a single vacutainer of NaF/Na 2 EDTA can be used safely for both glucose and HbA1c assays.

Analisa Kadar HbA1c Darah Vena dengan Antikoagulan EDTA dan Heparin Menggunakan Metode Imunofluoresens

“…14 Aditif ini digunakan dalam pengujian hematologi karena bertindak untuk mengawetkan komponen seluler darah, pada pemeriksaan ini bisa menggunakan tabung heparin. 15 Heparin adalah antikoagulan aditif yang direkomendasikan dalam kimia klinis. Antikoagulan aditif heparin, yaitu terdiri dari mucoitin polysulfuric acid yang sering digunakan dalam jumlah kecil sebagai antikoagulan yang efektif tanpa efek signifikan pada banyak determinan.…”