Evaluation by different diagnostic techniques of bovine abortion associated with Neospora caninum in Spain

Pereira-Bueno, J.; Quintanilla-Gozalo, A.; Pérez-Pérez, V.; Espi-Felgueroso, A.; Álvarez‐García, Gema; Collantes-Fernández, Esther; Ortega‐Mora, Luis Miguel

doi:10.1016/s0304-4017(02)00361-8

Cited by 55 publications

(33 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar findings were described by nested PCR where 80% and 57.7% (MEDINA et al, 2006;YAO et al, 2009) of aborted fetuses were positive for N. caninum, in Mexico and China, respectively. However, other studies (PEREIRA-BUENO et al, 2003;RAZMI et al, 2006RAZMI et al, , 2010SAGER et al, 2001) have described values that ranged from 11.9 to 33%. Thus, the results of this investigation are in agreement with the diagnosis of N. caninum-associated abortions in dairy cattle worldwide, where it was demonstrated that 12 to 45% of aborted fetuses are infected by this protozoan parasite (DUBEY, 2005).…”

Section: Discussionmentioning

confidence: 89%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

The aim of the present study was to investigate the occurrence of N. caninum associated with abortions of dairy cattle from Santa Catarina state, southern Brazil by using enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and polymerase chain reaction (PCR). Blood from dairy cows that aborted along with intrathoracic fluid and tissue samples (brain, heart, liver, and lung) from their fetuses were collected and used for serology; PCR, histopathological, and immunohistochemistry (IHC) evaluations were also conducted. Twenty-one cows (51.2%) out of 41, and eight fetuses (26.7%) out of 30 were ELISA (HerdCheck, IDEXX) positive for N. caninum. Dams > 36 months of age had a higher risk of being serum positive than younger animals. PCR and IHC revealed that 38.8% (14/36) and 25.0% (9/36) of the fetuses were positive for N. caninum, respectively for each of the tests. Seropositive cows had a higher frequency of fetuses that were also positive by either intrathoracic fluid, PCR, or IHC. In summary, the present study observed a high frequency of N. caninum in abortions from dairy cows from southern Brazil, with a higher N. caninum prevalence found in cows that were older than 36 months. In addition, serology, PCR, and IHC should be used all together for better diagnosis of neosporosis in cattle.Keywords: Neosporosis, fetuses, lesion, serology, cattle. ResumoO objetivo deste estudo foi avaliar a ocorrência de N. caninum associado a abortamentos em vacas de leite do estado de Santa Catarina, sul do Brasil pelo uso das técnicas de ELISA (HerdCheck, IDEXX), reação em cadeia pela polimerase (PCR) e imunohistoquímica (IHC). O sangue das vacas leiteiras que abortaram, bem como, o líquido intratorácico e amostras de tecidos (cérebro, coração, fígado e pulmão) de seus fetos foram coletados e usados para sorologia, PCR (Np21+ e Np6+), e IHC. Vinte e uma vacas (51,2%) de um total de 41, bem como, oito fetos (26,7%) de um total de 30 foram positivos no ELISA (IDEXX) para N. caninum. As vacas > 36 meses de idade tiveram um maior risco de serem soropositivas do que os animais mais jovens. PCR e IHC revelaram que 38,8% e 25,0% dos fetos foram positivos para N. caninum, respectivamente para cada um dos testes. As vacas soropositivas tiveram uma maior frequência de fetos que também foram positivos no fluído intratorácico, na PCR ou na IHC. Em resumo, o presente estudo observou uma alta frequência de N. caninum em abortos de vacas leiteiras na região estudada, com maior prevalência de N. caninum em vacas com mais de 36 meses de idade. Além disso, sorologia, PCR e IHC deveriam ser utilizadas conjuntamente para melhor diagnóstico de neosporose em bovinos.

show abstract

Section: Discussionmentioning

confidence: 89%

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Macedo

Miura

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

show abstract

“…PCR methods generally have a higher sensitivity than IHC methods and also a high specificity (van Maanen et al 2004). Fair agreement has been observed between both probes (Pereira-Bueno et al 2003, Medina et al 2006. Several PCRbased methods have been developed in the last few years targeting the parasite ITS1 region (Holmdahl and Mattsson 1996) and the repeated Neospora-specific Nc5 sequence (Muller et al 1996) with different modifications, such as nested or seminested PCR test, in an attempt to increase the sen- sitivity and specificity of the technique.…”

Section: Parasite Detectionmentioning

confidence: 99%

“…Infected cows of any age may abort from 3 months gestation to term, with most abortions occurring between 5 to 7 months of gestation (Pereira-Bueno et al 2003, Dubey 2005, and a cow may abort in successive gestations. Foetuses may die in utero and be reabsorbed, mummified, and/or autolysed (Dubey 2005).…”

Section: Transmission and Clinical Signsmentioning

confidence: 99%

Diagnosis of bovine neosporosis: Recent advances and perspectives

Ortega‐Mora

Fernández-García

Gómez-Bautista

2006

Acta Parasitologica

View full text Add to dashboard Cite

Neospora caninum is considered a major cause of abortion in cattle. Appropriate techniques for diagnosis of bovine neosporosis, both in vivo and in aborted foetuses, have been developed in the last ten years and some of them are commercially available. For diagnosis in live animals, detection of antibodies in serum or milk has been shown to be the best option both at the herd and the individual level. These techniques are excellent tools to examine N. caninum-associated abortion problems and to adopt some basic herd-control measures. Concerning foetal diagnosis, detection of compatible lesions by histological examination and parasites by PCR in brain (as well as heart and liver) are the best choices. Diagnostic criteria to distinguish foetal infection and Neospora-associated abortion are based not only on the demonstration of the parasite in the foetus but also on the extent and severity of the lesions in the foetus, foetal age and the assessment of neosporosis at the herd level. In the near future, new tools to diagnose infection should help to detect animals with parasite reactivation by testing the immune response to stage-specific antigens and lead to the development of molecular typing methods to characterise different parasite isolates. Finally, uniform diagnostic procedures need to be established between laboratories and countries in order to standardise result interpretation. The role of National or Regional Reference Laboratories is essential in countries or regions where control programmes for the disease are being developed.

show abstract

“…As differences have been reported in the tissue distribution of T. gondii and N. caninum Pereira-Bueno et al 2002) we investigated this aspect using the aborted lamb tissue samples. Samples for brain, heart and cord, where available, were collected from N. caninum-positive lambs and analysed for N. caninum presence, a similar number of T. gondii-positive lambs tissues were compared.…”

Section: Tissue Distribution Of Neospora Caninum In Lambsmentioning

confidence: 99%

The prevalence ofNeospora caninumand co-infection withToxoplasma gondiiby PCR analysis in naturally occurring mammal populations

et al. 2005

View full text Add to dashboard Cite

Neospora caninumandToxoplasma gondiiare closely related intracellular protozoan parasites associated with bovine and ovine abortion respectively. Little is known about the extent ofNeospora/Toxoplasmaco-infection in naturally infected populations of animals. Using nested PCR techniques, based on primers from the Nc5 region ofN. caninumand SAG1 forT. gondii, the prevalence ofN. caninumand its co-infection withT. gondiiwere investigated in populations ofMus domesticus,Rattus norvegicusand aborted lambs (Ovis aries). A low frequency of infection withN. caninumwas detected in theMus domesticus(3%) andRattus norvegicus(4·4%) populations. A relatively high frequency of infection withN. caninumwas detected in the brains of aborted lambs (18·9%). There was no significant relationship betweenN. caninumandT. gondiico-infection. Investigation of the tissue distribution ofNeospora, in aborted lambs, showed thatNeosporacould not be detected in tissues other than brain and this was in contrast toToxoplasmawhere the parasite could be frequently detected in a range of tissues.

show abstract

Evaluation by different diagnostic techniques of bovine abortion associated with Neospora caninum in Spain

Cited by 55 publications

References 31 publications

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Occurrence of abortions induced by Neospora caninum in dairy cattle from Santa Catarina, southern Brazil

Diagnosis of bovine neosporosis: Recent advances and perspectives

The prevalence ofNeospora caninumand co-infection withToxoplasma gondiiby PCR analysis in naturally occurring mammal populations

Contact Info

Product

Resources

About