“…After 4 washings over 1 h, the blots were incubated with horse radish peroxidase (POD)-conjugated GAH IgG and IgM (Dianova, Hamburg, FRG, dilution 1 : 4,000), washed again 4 times and developed with peroxidase substrate (20 mg 3-amino-9-ethylcarbazole, 2.5 ml N, N, dimethylformamid, 50 p1 30% H202 in 50 ml 10 mM tris, pH 8.0). After [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] min, the reactions were stopped with 0.02% NaN3 in washing buffer.…”