Evaluation inhibitory activity of catechins on trypsin by capillary electrophoresis–based immobilized enzyme microreactor with chromogenic substrate

Zhang, Hao; Lü, Min; Jiang, Hui; Wang, Xu; Yang, Feng‐Qing

doi:10.1002/jssc.202000132

Cited by 14 publications

(5 citation statements)

References 26 publications

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“…The same classes of polyphenols inhibiting amylase and lipase activity are also able to inhibit the activity of digestive proteases [5, 22]. The relationship between inhibition of digestive protease activity and health benefits has been discussed several times in the context of the effects of polyphenols from wine and tea [23, 24]. Certain berry polyphenol components inhibit protease activities at levels which could affect protein digestion in the gastrointestinal tract [3].…”

Section: Inhibition Of Protein Metabolismmentioning

confidence: 99%

Plant Polyphenols as Inhibitors of Hydrolases are Regulators of Digestion

Melzig¹

2023

Complement Med Res

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Background There are numerous reports on the use of polyphenol-containing foods and various medicinal plant preparations for the prophylaxis and therapy of metabolic diseases, such as metabolic syndrome and diabetes mellitus, respectively. One unifying aspect to the effect of these natural compounds is their ability to inhibit digestive enzymes, which is the focus of this review. Summary Polyphenols inhibit non-specifically hydrolytic enzymes included in the digestion process, e.g. amylases, proteases, lipases. By that the digestion process is protracted with different consequences as result of the incomplete absorption of monosaccharides, fatty acids and amino acids as well as for the enhanced availability of substrates for the microbiome in ileum and colon. The resulting postprandial blood concentration of monosaccharides, fatty and amino acids is lowered and by that different metabolic pathways proceed more slowly. As another positive result, polyphenols can also modulate the microbiome and thus mediate additional beneficial health effects. Key Messages Many medicinal plants possess a broad spectrum of different polyphenols, thereby mediating the non-specific inhibition of all hydrolytic enzyme activities in the gastrointestinal digestive process. As a consequence of the slowing down of digestive processes, risk factors for the development of metabolic disorders are reduced and the health of the patients with metabolic syndrome improves.

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Section: Inhibition Of Protein Metabolismmentioning

confidence: 99%

Plant Polyphenols as Inhibitors of Hydrolases are Regulators of Digestion

Melzig¹

2023

Complement Med Res

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“…An interesting application of CE beyond the characterization of food samples coupling separation and bioactivity assay of phenolic compounds has recently been presented. The approach is based on the use of CE together with an on‐line immobilized trypsin microreactor to allow the inhibitory activity of phenolic acids and green tea polyphenols [61, 62]. In this method, trypsin is immobilized in the inner wall of the separation capillary and a chromogenic substrate is employed to allow detection.…”

Section: Secondary Metabolitesmentioning

confidence: 99%

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period February 2019–February 2021

et al. 2021

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This work presents a revision of the main applications of capillary electromigration methods in food analysis and Foodomics. Articles that were published during the period February 2019‐February 2021 are included. The work shows the multiple CE methods that have been developed and applied to analyze different types of molecules in foods. Namely, CE methods have been applied to analyze amino acids, biogenic amines, carbohydrates, chiral compounds, contaminants, DNAs, food additives, heterocyclic amines, lipids, secondary metabolites, peptides, pesticides, phenols, pigments, polyphenols, proteins, residues, toxins, vitamins, small organic and inorganic compounds, as well as other minor compounds. The last results on the use of CE for monitoring food interactions and food processing, including recent microchips developments and new applications of CE in Foodomics, are discussed too. The new procedures of CE to investigate food quality and safety, nutritional value, storage and bioactivity are also included in the present review work.

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“…It was validated with the use of the trypsin inhibitor benzamidine and by comparison with a UV‐Vis spectrophotometric method. Finally, four catechins and three polyphenol extracts from tea were tested for their trypsin inhibitory activity [72].…”

Section: Heterogeneous Enzyme Reaction Studiesmentioning

confidence: 99%

Overview of in‐capillary enzymatic reactions using capillary electrophoresis

2021

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This review summarizes the research that has recently been performed on in‐capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At‐inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non‐magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.

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Evaluation inhibitory activity of catechins on trypsin by capillary electrophoresis–based immobilized enzyme microreactor with chromogenic substrate

Cited by 14 publications

References 26 publications

Plant Polyphenols as Inhibitors of Hydrolases are Regulators of Digestion

Plant Polyphenols as Inhibitors of Hydrolases are Regulators of Digestion

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period February 2019–February 2021

Overview of in‐capillary enzymatic reactions using capillary electrophoresis

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