Evaluation Intra-testicular Injection of Platelet-Rich Plasma in Restoration Male Rat Fertility Affected By Cisplatin

Abstract

Cisplatin is considered the first-line chemotherapy, reducing its cytotoxicity is still unmet need. Platelet-rich plasma (PRP) has a potential effect on tissue repair through regeneration and differentiation of tissue progenitor cells. Here, we used PRP as a biomaterial to protect and regenerate testicular tissue against cisplatin toxicity. PRP was applied locally using intra-testicular injection as a single dose per week for three following the cisplatin treatment (intra-peritoneum injection). Cisplatin treatment alone and PRP intra-testicular injection alone were used as control groups. The results show that PRP treatment was able to restore sperm counts and sperm morphology following cisplatin exposure. PRP treatment significantly reintited reproductive hormonal balance in affected rats with cisplatin. The results also showed that PRP treatment minimized histological damaged in testicular tissue resulting by the cisplatin, however, also increasing the number of spermatocytes in all stages of spermatogenesis. As well as elevation in the number of leydig cells and thickness of interstitial tissue was restored. This indicates the potential role of PRP in tissue regeneration in addition to tissue repair which may provide a promising use of PRP in different aspects related to chemotherapy.