Evaluation of 11 single‐locus and seven multilocusDNAbarcodes inLamiumL. (Lamiaceae)

Abstract

The aim of this work was to evaluate the suitability of selected DNA regions in the barcoding of plants, based on the species belonging to the genus Lamium (Lamiaceae). For this purpose, nine chloroplast barcodes, that is, accD, matK, rbcL, rpoA, rpoB, rpoC1, rpoC2, trnH-psbA, trnL-trnF, as well as ITS nuclear region, and intron of mitochondrial nad5 gene were tested. Among the single-locus barcodes, most effective in the identification of Lamium species was the trnH-psbA spacer and matK gene. The high level of variability and resolving power was also observed in the case of rpoA and rpoC2 genes. Despite the high interspecies variability of ITS region, it turned out to be inapplicable in Lamium identification. An important disadvantage of ITS as a barcode is a limitation of its use in polyploid plants, samples contaminated with fungal material or samples with partially degraded DNA. We have also evaluated five-two-locus and two-three-locus barcode regions created from a combination of most effective single loci. The best-performing barcode combinations were matK + trnH-psbA and matK + rpoA. Both of them had equally high discriminative power to identify Lamium species.

Nutlet micromorphology and its taxonomic utility in Lamium L. (Lamiaceae)

“…laevigatum a separate taxon. This result is consistent with the analysis of variation of barcoding sequences in the genus Lamium, which also indicated the distinctiveness of this subspecies (Krawczyk et al 2014;.…”

“…The results of phylogenetic studies showed that L. galeobdolon did not form a separate phylogenetic line in relation to the genus Lamium and thereby confirmed that the presence of L. galeobdolon in Lamium was justified . Molecular studies confirmed a close relationship of L. flexuosum, L. galeobdolon and L. orvala (Krawczyk et al 2014). The infrageneric classification based on macromorphological features also emphasizes the similarity of L. galeobdolon and L. flexuosum which constitute the subgenus Galeobdolon, while L. orvala belongs to the monospecific subgenus Orvala (Mennema 1989).…”

Identification of Species in <i>Tripterygium</i> (Celastraceae) Based on DNA Barcoding

“…Recently, several candidate DNA regions, such as the internal transcribed spacer 2 (ITS2), megakaryocyte-associated tyrosine kinase (matK), a large subunit of the ribulose-1,4-bisphosphate carboxylase gene (rbcL), and the intergenic spacer photosystem II protein D1-tRNA-His (psbA-trnH), or a combination of them have been popularly used as suitable DNA barcodes in plants. [18][19][20][21] Lahaye et al recommended matK as a universal DNA barcode for flowering plants. 17) The Plant Working Group of the Consortium for the Barcode of Life (CBOL) officially proposed that rbcL and matK were able to serve as core barcodes, and psbA-trnH or ITS regions could serve as supplemental loci for differentiating plant species.…”

Plastid and nuclear genomic resources of a relict and endangered plant species: Chamaedaphne calyculata (L.) Moench (Ericaceae)

“…Mean pairwise identity for coding regions was 96.6%. The nucleotide variation in the gene coding Acetyl-CoA carboxylase (accD) is not surprising, since it has proven its usefulness many times in plant barcoding studies (Newmaster et al, 2008;Krawczyk et al, 2014). In coding regions, the total number of nonsynonymous and synonymous mutations was similar at 354 and 375, respectively ( Figure 5).…”