Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae)

Zhang, Weiwei; Duan, Xiao-Juan; Huang, Hongbo; Zhang, Yi; Wang, Bin‐Gui

doi:10.1007/s10811-006-9115-x

Cited by 98 publications

(65 citation statements)

References 26 publications

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“…The total phenolic contents of macroalgae were determined by using Folin-Ciocalteu assay (Zhang et al 2007) in 96-well plates. Methanol extracts (10 mg mL -1 ) were loaded into 96-well plate containing 90 lL of deionized water and 10 lL 2N FolinCiocalteu's reagent (Sigma-Aldrich) in each well.…”

Section: Determination Of Dpph Radical Scavenging Activitymentioning

confidence: 99%

“…Macroalgae are prolific sources for numerous natural compounds with promising potential for both prevention (Lee et al 2013) and treatment of several diseases including cancer (Hiroishi et al 2001;RademakerLakhai et al 2005) and diabetes (Nwosu et al 2011). Many studies have attributed beneficial effects of macroalgae to their high antioxidant capacity (Zhang et al 2007;Zubia et al 2009;Murugan and Iyer 2013) thus naming their consumption ''antioxidant phytomedicine'' (Scalbert et al 2005;Yuan and Walsh 2006;Gupta and Abu-Ghannam 2011;Nwosu et al 2011). Dietary antioxidant intake and decreased oxidative stress are known to reduce the risk of type II diabetes (Valko et al 2004) and cancer (Montonen et al 2004) as both conditions are associated with oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Macroalgae of Izmir Gulf: Dictyotaceae exhibit high in vitro anti-cancer activity independent from their antioxidant capabilities

2016

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In this study, 24 marine macroalgae collected from the coastline of Izmir Gulf were examined for their antioxidant activities and their effects on cell proliferation. Crude extracts were obtained from samples with cold methanol extraction. Antioxidant activity was evaluated as 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and total phenolic content (TPC); growth inhibitory effects of the extracts were determined by using WST-8. Amongst the species, Polysiphonia denuata (Rhodophyta) and Cystoseira species (Phaeophyceae) have been noticed by their high DPPH radical scavenging activities and TPCs. As expected, there was a strong correlation between these tests. Dictyota dichotoma (Phaeophyceae) showed the highest anti-cancer activity on MCF-7 cells with an IC 50 of 17.2 ng mL -1 . Flow cytometry analyses demonstrated that D. dichotoma methanolic extract strongly induced apoptosis.This extract exhibited moderate viability inhibition on MCF10A cells (IC 50 : 49.3 ng mL -1 ), suggesting a potential use of the extracts or its compounds for cancer therapy. There was no correlation between anticancer potential and antioxidant content of the extracts.

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Section: Determination Of Dpph Radical Scavenging Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Macroalgae of Izmir Gulf: Dictyotaceae exhibit high in vitro anti-cancer activity independent from their antioxidant capabilities

2016

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“…The radical cation was prepared by mixing a 7 mM ABTS stock solution with 2.45 mM potassium persulfate (1/1, v/v) and leaving the mixture for [4][5][6][7][8][9][10][11][12][13][14][15][16] h until the reaction was completed and the absorbance was stable. The ABTS .+ solution was diluted with ethanol to an absorbance of (0.70 暲0.05) at 734 nm for measurements.…”

Section: Abts Radical Cation Scavenging Assaymentioning

confidence: 99%

“…Several bioactive metabolites produced by cyanobacteria and algae have been discovered by screening programs, employing target organisms quite unrelated to those for which the metabolites evolved [4] .…”

Section: Introductionmentioning

confidence: 99%

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Shanab

Mostafa

Shalaby

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

166

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“…These polar substances were found in extracts of different red, brown and green seaweeds (Matsukawa et al, 1997;Anggediredja et al, 1997;Lim et al, 2002;Santoso et al, 2004;Zhang et al, 2007;Shanab, 2007;Shalaby, 2008) as well as in micro algae and cyanobacterial species Reddy et al, 2003;Benedetti et al, 2004;Shalaby, 2004) these substances mainly include phycocyanin pigment, sulphated polysaccharides and phenolic compounds which are largely present in most macro, micro and cyanobacterial species which exhibited pronounced antioxidant activity. Non-polar antioxidant substances were recorded also in microalgae as Chlorella vulgaris, Dunaliella salina (Nirupama, 2004;Murthy et al, 2005;Zhang-Bao et al, 2004) in cyanobacteria (Shalaby, 2004) in addition to the different seaweed species (Krinsky, 1989;Hyun et al, 2003;Xi et al, 2003;Sook et al, 2004;Yuan et al, 2005;Shanab, 2007;Shalaby, 2008).…”

Section: Nacl (M)mentioning

confidence: 99%

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

Shalaby¹,

Shanab²,

Singh³

2010

J. Med. Plants Res.

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Cultivation of Spirulina platensis under salt stress conditions (0.02 M as control), 0.04 and 0.08 M NaCl led to a remarkable alteration of algal metabolism as well as an enhancement or induction of biologically active compounds. Concerning algal growth, salt stress caused a decrease in dry weight, chlorophyll a content as well as certain xanthophylls (neoxanthin and violaxanthin) while β-carotene production was stimulated especially at higher salt concentrations. Biochemical analysis of salt stressed algal revealed that lipid content was slightly increased together with certain saturated and unsaturated fatty acids especially the polyunsaturated ones (γ-inolenic acid, omega 3 fatty acid). Electrophoretic analysis of soluble protein pointed out that certain high molecular weight protein bands were not detected comparing with the protein marker. Five new protein bands of molecular weights 190, 158, 113, 77 and 28 KDa were recorded, in addition to an increase in the intensity of 6 already existing bands. Phosphate buffer and water extracts of the algal exhibited antiviral activities against both Hepatitis-A-virus-type-MBB (HAV-MBB strain, RNA virus) and Herpes simplex-virus-type-1 (HSV-1, DNA virus). Water extracts were found to be more effective than phosphate buffer extracts in inducing antiviral activities (98%) especially against HSV-1 virus. The same water extract of the salt stressed algal demonstrated higher anticoagulating activity compared with those of heparin and the positive control measured by clotting time assay. Antioxidant activity of the algal successive extracts against 2, 2 diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (ethylbenzthiazoline-6-sulfonic acid) radical methods revealed moderate antioxidant activity of the non-polar algal extracts (petroleum ether) which were doubled with increasing extract concentration. The lowest activity was recorded by the partially polar (ethyl acetate) algal extract of both concentrations at all salinity levels. While the polar extracts (ethanol and water) showed higher antioxidant activities which were doubled with increasing extract concentration. Ethanolic algal extract (100 µg/ ml at 0.08 M NaCl) exhibited the highest antioxidant activity compared with those of the synthetic antioxidant butylated hydroxy anisol as standard (85.0, 89.9 and 86.0, 91.8% respectively).Key words: Spirulina platensis, antioxidant-antivirus, biochemical studies, salt stress. INTRODUCTIONMany cyanobacteria and micro algae were considered as a natural source of various biologically and pharmacologically active compounds with structurally complex molecules which are difficult or impossible to be *Corresponding author. E-mail: dremad2009@yahoo.com.produced by chemical synthesis (Smith and Doan, 1999). Genus Spirulina has gained an importance and international demand for its high phytonutrients value and pigments which have applications in healthy foods, animal feed, therapeutics and diagnostics (Becker, 1994;Vonshak and Tomaselli, 2000). Spirulina has been used as food and nutritional ...

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Evaluation of 28 marine algae from the Qingdao coast for antioxidative capacity and determination of antioxidant efficiency and total phenolic content of fractions and subfractions derived from Symphyocladia latiuscula (Rhodomelaceae)

Cited by 98 publications

References 26 publications

Macroalgae of Izmir Gulf: Dictyotaceae exhibit high in vitro anti-cancer activity independent from their antioxidant capabilities

Macroalgae of Izmir Gulf: Dictyotaceae exhibit high in vitro anti-cancer activity independent from their antioxidant capabilities

Aqueous extracts of microalgae exhibit antioxidant and anticancer activities

Salt stress enhancement of antioxidant and antiviral efficiency of Spirulina platensis

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