Evaluation Of 8-Hydroxy-2-Deoxyguanosine And Malondialdehyde Levels In First-Trimester Miscarriage: A Prospective Cohort Study

Abstract

Objective: This study was aimed to evaluate 8-hydroxy-2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels in patients with first trimester miscarriage. Methods: This prospective cohort study included 35 women, who were diagnosed with miscarriage (patient group) and 35 healthy pregnant women below 14 weeks of gestation (control group), who did not report any complaints. 8-OHdG levels were measured with a competitive ELISA (Enzyme-Linked ImmunoSorbent Assay) high sensitivity kit and MDA levels were also measured in both groups. Results: There wasn't difference in terms of complete blood count, thyroid-stimulating hormone (TSH), homocysteine values, parity, age, body mass index (BMI) and gestational age between the groups. Serum 8-OHdG and MDA levels were significantly higher in the patient group (p=0.001). A moderate positive significant correlation between the gestational week and MDA values and a weak but significant positive correlation between 8-OHdG values and gestational week were detected in the patient group.

Assessment of DNA damage in relation to heavy metal induced oxidative stress in females with recurrent pregnancy loss (RPL)

“…Enhanced production of 8-OHdG in RPL group is reflective of the DNA damage accountable for pregnancy termination in these patients. The data obtained here, is in accordance with previous finding ( Gunduz et al, 2020 ).…”

Recurrent miscarriage and factors that effects its: A review

“…Oxidative stress (OS) occurs due to an elevation in the levels of free radicals, production, and/or reactive oxygen species (ROSs), coupled with a decline in the biological system's ability to efficiently e liminate the reactive intermediates or repair the resulting damage. Because OS plays a pathophysiologic role in the placental enzymes and fetus, increased free radical activity is seen in pregnant women with conditions like preeclampsia, spontaneous abortion, and intrauterine growth restriction [27].…”

Oxidative/nitrosative stress increased the risk of recurrent pregnancy loss–Taiwan Recurrent Pregnancy Loss and Environmental Study (TREPLES)

“…Most studies point out that 8-OHdG is an oxidative base adduct and a predictive biomarker for oxidative induce DNA damage in the sperm [ [25] , [26] , [27] , [28] ]. A prospective cohort study suggested that 8-OHdG and MDA influence the etiopathogenesis of first-trimester miscarriage [ 29 ].…”