Evaluation of 9 rapid diagnostic tests for screening HIV infection, in Lomé, Togo

Dagnra, Anoumou; Dossim, Sika; Salou, Mounérou; Nyasenu, T.; Ali-Edje, K.; Ouro-Medeli, Alassane; Doufan, M.; Ehlan, Amivi; Prince-David, Mireille

doi:10.1016/j.medmal.2014.10.007

Cited by 14 publications

(12 citation statements)

References 10 publications

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“…Although not the focus of our study, these data confirm previous studies evaluating the laboratory performance of the SD BIOLINE HIV/Syphilis Duo which found sensitivities and specificities for HIV antibody detection in sera ranging from 97.9–100% and 99.0–100%, respectively and T. pallidum antibody detection sensitivities and specificities ranging from 93.0–100% and 96.0–100%, respectively [7, 8, 10, 20, 22–25]. Although no previous evaluations of the First Response HIV/Syphilis Combo Card Test have been published, our results are well within the ranges found for other dual HIV/syphilis point of care tests using sera with sensitivities and specificities for HIV antibody detection ranging from 95.7–100% and 91.9–100%, respectively, and for T. pallidum antibody detection ranging from 46.4–97.0% and 93.8–100%, respectively [7–10, 13, 20, 22–27].…”

Section: Discussionsupporting

confidence: 90%

“…Although no previous evaluations of the First Response HIV/Syphilis Combo Card Test have been published, our results are well within the ranges found for other dual HIV/syphilis point of care tests using sera with sensitivities and specificities for HIV antibody detection ranging from 95.7–100% and 91.9–100%, respectively, and for T. pallidum antibody detection ranging from 46.4–97.0% and 93.8–100%, respectively [7–10, 13, 20, 22–27]. …”

Section: Discussionsupporting

confidence: 85%

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Laboratory Evaluation of a Smartphone-Based Electronic Reader of Rapid Dual Point-of-Care Tests for Antibodies to Human Immunodeficiency Virus and Treponema pallidum Infections

et al. 2017

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Background Dual point of care tests for antibodies to human immunodeficiency virus (HIV) and Treponema (T.) pallidum allow for same-day testing and treatment and have been demonstrated to be cost-effective in preventing the adverse outcomes of HIV infection and syphilis. By recording and transmitting data as they are collected, electronic readers address challenges related to the decentralization of point of care testing. Methods We evaluated a smartphone-based electronic reader using 201 sera tested with two dual rapid tests for detection of antibodies to HIV and T. pallidum in Los Angeles, USA and Lima, Peru. Tests were read both visually and with the electronic reader. Enzyme immunoassay followed by Western blot and T. pallidum particle agglutination were the reference tests for HIV and T. pallidum, respectively. Results The sensitivities of the two rapid tests for detection of HIV were 94.1% and 97.0% for electronic readings. Both tests had specificity of 100% for detection of HIV by electronic reading. The sensitivities of the two rapid tests for detection of T. pallidum were 86.5% and 92.4% for electronic readings. The specificities for detection of T. pallidum were 99.1% and 99.0% by electronic reading. There were no significant differences between the accuracies of visual and electronic readings, and the performance did not differ between the two study sites. Conclusions Our results show the electronic reader to be a promising option for increasing the utility of point of care testing programs.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 85%

Laboratory Evaluation of a Smartphone-Based Electronic Reader of Rapid Dual Point-of-Care Tests for Antibodies to Human Immunodeficiency Virus and Treponema pallidum Infections

et al. 2017

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“…Results can be read in less than 1 min. Previous studies of the INSTI HIV-1/HIV-2 antibody test found it to be highly sensitive, specific, and easy to use (5)(6)(7)(8)(9)(10)(11). The INSTI Multiplex assay was developed recently, and no published data on its accuracy, feasibility, or acceptability exist yet.…”

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confidence: 99%

“…Previous studies evaluated dual tests for antibodies to HIV and T. pallidum, including the MedMira Multiplo (MedMira; Halifax, Nova Scotia, Canada), the SD BIOLINE HIV/Syphilis Duo test (Standard Diagnostics; Gyeonggi-do, Republic of Korea), and the Chembio DPP HIV-syphilis assay (Chembio Diagnostics; Medford, NY) (7,(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). These dual tests demonstrated sensitivities and specificities for HIV antibody detection ranging from 93.8% to 100% and 91.9% to 100%, respectively.…”

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confidence: 99%

Laboratory Evaluation of a Point-of-Care Downward-Flow Assay for Simultaneous Detection of Antibodies to Treponema pallidum and Human Immunodeficiency Virus

et al. 2016

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dCombining the detection of syphilis and HIV antibodies into one point-of-care test integrates syphilis screening into already existing HIV screening programs, which may be particularly beneficial in settings such as antenatal care. Using the INSTI Multiplex downward-flow immunoassay, we tested 200 stored serum samples from high-risk patients enrolled in a longitudinal study on HIV infection and syphilis in Peruvian men who have sex with men and transgender women. This rapid assay detected HIV and Treponema pallidum serum antibodies with sensitivities of 100% (95% confidence interval [CI], 95.9% to 100%) and 87.4% (95% CI, 81.4% to 92.0%), respectively, and specificities of 95.5% (95% CI, 89.9% to 98.5%) and 97.0% (95% CI, 84.2% to 99.9%), respectively (n ‫؍‬ 200). The sensitivity for syphilis antibody detection was higher in patients with a rapid plasma reagin titer of >1:8 (97.3%) than in those with a titer of <1:4 (90%) or a nonreactive titer (66.7%). While syphilis, an infection caused by the spirochete Treponema pallidum, is a curable disease, it is estimated that there are 5.6 million new syphilis infections in adults annually (1) compared to approximately 2 million new HIV infections (2). Although new syphilis infections are more common, HIV screening has become a priority in low-and middle-income countries. Syphilis frequently presents atypically, which can make it difficult to clinically differentiate from other sexually transmitted infections; thus, effective diagnostic tests are crucial for correctly identifying and managing syphilis infections (3). Highly accurate diagnostic tests for HIV infection and syphilis exist, but they are often inaccessible to people living in areas with the highest burden of disease. Point-of-care tests provide an opportunity to reach those populations through tests that are inexpensive, easy to perform, and rapid, which enables same-day testing and treatment. Combining the detection of syphilis and HIV antibodies into one point-of-care test integrates syphilis screening into already existing HIV screening programs, which might be particularly beneficial in settings such as antenatal care (4).The INSTI Multiplex downward-flow immunoassay (bioLytical Laboratories, Inc., Canada) is for the simultaneous detection of HIV and T. pallidum antibodies. Results can be read in less than 1 min. Previous studies of the INSTI HIV-1/HIV-2 antibody test found it to be highly sensitive, specific, and easy to use (5-11). The INSTI Multiplex assay was developed recently, and no published data on its accuracy, feasibility, or acceptability exist yet.Using the INSTI Multiplex, we tested 200 stored (Ϫ80°C) serum samples from high-risk patients enrolled in a longitudinal study on HIV infection and syphilis in Peruvian men who have sex with men and transgender women (12). Genital lesions are a sign of primary syphilis, and 14 of the 200 serum samples were from patients who had primary syphilis, confirmed by T. pallidum DNA detection using PCR (12). The sera were tested for HIV and T. pallidum ant...

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“…Most new HIV-1 infections will occur in developing countries, such as in sub-Saharan Africa where non-B subtypes are the most prevalent. We were interested by the evaluation of HIV rapid diagnostic tests in Togo [1], a country reporting generalized HIV epidemics with a prevalence in adults ranging at 2.3% (HIV prevalence in 2013; http://www.unaids.org/en/regionscountries/countries/togo).…”

mentioning

confidence: 99%

Performance of rapid HIV-1/HIV-2 INSTI® on plasma and capillary blood for serological diagnosis of non B subtypes and circulating recombinant forms of HIV-1 in Gabon

Ndjoyi-Mbiguino

Nzengui

Robin

et al. 2015

Médecine et Maladies Infectieuses

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Evaluation of 9 rapid diagnostic tests for screening HIV infection, in Lomé, Togo

Cited by 14 publications

References 10 publications

Laboratory Evaluation of a Smartphone-Based Electronic Reader of Rapid Dual Point-of-Care Tests for Antibodies to Human Immunodeficiency Virus and Treponema pallidum Infections

Laboratory Evaluation of a Smartphone-Based Electronic Reader of Rapid Dual Point-of-Care Tests for Antibodies to Human Immunodeficiency Virus and Treponema pallidum Infections

Laboratory Evaluation of a Point-of-Care Downward-Flow Assay for Simultaneous Detection of Antibodies to Treponema pallidum and Human Immunodeficiency Virus

Performance of rapid HIV-1/HIV-2 INSTI® on plasma and capillary blood for serological diagnosis of non B subtypes and circulating recombinant forms of HIV-1 in Gabon

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