Evaluation of a 2% chlorhexidine gluconate in 70% isopropyl alcohol skin disinfectant

Adams, Debra; Quayum, M.H.; Worthington, Tony; Lambert, Peter A.; Elliott, Tom

doi:10.1016/j.jhin.2005.05.015

Cited by 109 publications

(72 citation statements)

References 5 publications

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“…PBS (250 ml) was added to each well and the plate was sonicated at 50 Hz in a water bath for 30 min at room temperature. The biofilms were recovered from the wells using a scrape and wash procedure (Adams et al, 2005) and the contents of the wells (10 ml) were mixed with molten Letheen broth. Following incubation of the set plates at 37 uC for 24 h, the minimum biofilm inhibition concentrations (MBICs) were determined as the lowest concentration to show growth below or equal to that of the control.…”

Section: Methodsmentioning

confidence: 99%

The synergy of berberine chloride and totarol against Staphylococcus aureus grown in planktonic and biofilm cultures

Guo

Zhao

et al. 2015

Journal of Medical Microbiology

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Staphylococcus aureus (S. aureus) is commonly associated with hospital-acquired infections and is known to form biofilms. Bacteria inside biofilms display an increased resistance to chemotherapeutics and host immune defences. Efficient antibiotics or combination therapy are urgently needed to treat patients with biofilm-associated MRSA infections. The objective of the current study was to evaluate the in vitro antimicrobial activities of totarol alone or in combination with berberine chloride (BBR) against S. aureus grown in planktonic and biofilm cultures. The synergistic antimicrobial effects between BBR and totarol were observed in all tested strains grown in biofilms using a chequerboard microdilution method, with the fractional inhibitory concentration index values ranging from 0.125 to 0.375. No antagonistic activity was observed in any of the strains tested in suspension or biofilm cultures. The synergistic activity against S. aureus biofilms was also corroborated by confocal laser scanning microscopy and adhesion assays. Moreover, the present study demonstrated that combination BBR and totarol treatment effectively decreased the formation of S. aureus biofilms by affecting extracellular genomic DNA release and polysaccharide intercellular adhesin expression. Subsequently, real-time reverse transcriptase PCR analysis revealed that the combination of BBR and totarol effectively inhibited the transcription of the biofilm-related genes sarA, cidA and icaA. These results suggest that the combination of totarol and BBR is momentous for the further development of a therapy protocol against S. aureus biofilms.

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Section: Methodsmentioning

confidence: 99%

The synergy of berberine chloride and totarol against Staphylococcus aureus grown in planktonic and biofilm cultures

Guo

Zhao

et al. 2015

Journal of Medical Microbiology

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“…[6], %70 izopropil alkol (IPA) içinde %2 klorheksidin glukonat (KHG) içeren Yapılan çalışmalar, alkol ile klorheksidin kombinasyonunun cilt antisepsisinde, alkol ve klorheksidini tek başına içeren preparatlara kıyasla daha iyi bir seçenek olabileceğini göstermektedir [17]. Taha ve ark.…”

Section: Tablo 2 Kantitatif Süspansiyon Test Sonuçlarıunclassified

“…Ancak Centers for Disease Control and Prevention bu amaçla klorheksidin bazlı preparatların kullanımını önermektedir. Mikrobiyal kolonizasyon ve sonrasında oluşabilecek sepsis riskini azaltmak amacıyla, cildin antimikrobiyal bir solüsyon ile 30 saniye boyunca dezenfekte edilmesi önerilmektedir [5][6][7][8][9]. Bu çalışmanın amacı, hastanelerde sık kullanılan beş antiseptik solüsyonun biyofilm oluşturan ve oluşturmayan S. epidermidis suşlarına karşı antibakteriyel etkinliğinin araştırılmasıdır.…”

Section: Introductionunclassified

Hastanelerde sık kullanılan bazı antiseptiklerin biyofilm oluşturan ve oluşturmayan Staphylococcus epidermidis suşlarına karşı antibakteriyel etkinliğinin araştırılması

GÜRPINAR¹

2017

Ankara Üniversitesi Eczacılık Fakültesi Dergisi

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“…1), is a clinically important biocide, disinfectant, and preservative which exists in oral rinses and skin cleansers, and, in small quantities, it is used as a preservative [22,23]. A limited number of published papers have been devoted hitherto to study the above antibacterial agents as biological coatings on polymeric supports [21,[24][25][26][27][28][29]. Zhang et al [21,24] immobilized bronopol entities on medicalgrade polyethylene (PE) [21] and PVC [24] by means of plasma treatment and reported excellent antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) strains.…”

Section: Introductionmentioning

confidence: 99%

“…Harnet et al [26] investigated the antibacterial effects obtained with chlorhexidine-functionalized polyelectrolyte films built on different types of suture materials and reported an inhibition of E. coli proliferation by 40% and 99% after 24 and 48 h incubation periods, respectively. Adams et al [27] gave an account of the enhanced antisepsis efficacy of 2% (w/v) chlorhexidine in 70% (v/v) isopropyl alcohol compared to the equal concentration of the same material in pure water used as a common skin disinfectant solution. Yao et al [28] reported a highly efficient antibacterial layer on PU fibrous membranes obtained from quaternary ammonium entities.…”

mentioning

confidence: 99%

An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

Asadinezhad

Novák

Lehocký

et al. 2010

Colloids and Surfaces B: Biointerfaces

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A B S T R A C TMedical-grade polyvinyl chloride was surface modified by a multistep physicochemical approach to improve bacterial adhesion prevention properties. This was fulfilled via surface activation by diffuse coplanar surface barrier discharge plasma followed by radical graft copolymerization of acrylic acid through surfaceinitiated pathway to render a structured high density brush. Three known antibacterial agents, bronopol, benzalkonium chloride, and chlorhexidine, were then individually coated onto functionalized surface to induce biological properties. Various modern surface probe techniques were employed to explore the effects of the modification steps. In vitro bacterial adhesion and biofilm formation assay was performed. Escherichia coli strain was found to be more susceptible to modifications rather than Staphylococcus aureus as up to 85% reduction in adherence degree of the former was observed upon treating with above antibacterial agents, while only chlorhexidine could retard the adhesion of the latter by 50%. Also, plasma treated and graft copolymerized samples were remarkably effective to diminish the adherence of E. coli.

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Evaluation of a 2% chlorhexidine gluconate in 70% isopropyl alcohol skin disinfectant

Cited by 109 publications

References 5 publications

The synergy of berberine chloride and totarol against Staphylococcus aureus grown in planktonic and biofilm cultures

The synergy of berberine chloride and totarol against Staphylococcus aureus grown in planktonic and biofilm cultures

Hastanelerde sık kullanılan bazı antiseptiklerin biyofilm oluşturan ve oluşturmayan Staphylococcus epidermidis suşlarına karşı antibakteriyel etkinliğinin araştırılması

An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

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