Evaluation of a combination of Citrus aurantium var. Dulcis essential oil and albendazole for the treatment of sheep gastrointestinal nematodes

Sousa, Dauana Mesquita; Campos, Nagilla RCL; Pereira, Juliana R. F.; Gomes, Matheus N; Silva, Carolina R; Cutrim-Júnior, Jose AA; Brito, Danilo RB; Sampaio, Romildo M; Sargison, Neil; Lifschitz, Adrián Luis; Costa-Júnior, Lívio Martins

doi:10.1016/j.vetpar.2023.109929

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…Mesquita-Sousa et al . [ 18 ] noted that Citrus aurantium var. is effective when used in combination.…”

Section: Resultsmentioning

confidence: 99%

Anthelmintic effects of Podang mango (Mangifera indica) fruit peel waste extract through in vivo application on Indonesian Etawa goat production and health

Prasetyo,

Rokana,

Baihaqi

et al. 2024

Vet World

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Background and Aim: The continuous use of anthelmintic drugs has led to global issues of resistance. One breakthrough to address this problem is the utilization of bio-anthelmintics derived from active compounds in agro-industrial waste. This in vivo study investigated the effectiveness of Podang mango (Mangifera indica L.) fruit peel waste extract for anthelmintic purposes, using concentrations up to 5%. Materials and Methods: This study included 28 Etawa crossbred goats aged 17 months. Goats were randomly assigned to four groups: A negative control, an aqueous fruit peel extract (AFPE) group at 2.5%, another AFPE group at 5%, and a positive control receiving ivermectin. Goats chosen had egg per gram (EPG) counts surpassing 1000 before exposure to Haemonchus contortus. For 7 days within a 9-week study, AFPE from Podang mangoes was given. On the 7th day, the positive control group was administered ivermectin. AFPE dosage relied on the average abomasum fluid per kilogram of animal weight. The feeding regimen consisted of concentrate and Pennisetum purpureum cv. Mott is customized for the nutritional needs of livestock. Data on feed consumption, digestibility, average daily gain, percentage reduction in fecal egg count, body condition score, and clinical parameters were collected throughout the study. Results: At higher treatment levels (AFPE), there was a greater reduction in both EPG and fecal egg counts. The expected and normal ranges were maintained for consumption and digestibility. While body weight increased, FAMACHA parameters showed a decrease. Compared to the negative control group, substantial disparities (p < 0.05) existed for hemoglobin, red blood cells, and hematocrit in both the positive control and the treatment groups. Blood urea nitrogen and creatinine, indicative of liver and kidney health, were within normal ranges. Conclusion: At a concentration of up to 5%, Podang mango waste extract (AFPE) can function as a substitute for traditional helminth medicines or bio-anthelmintics in goats, enhancing their production. Keywords: anthelmintic, feed additive, gastrointestinal helminth infection, goat, Haemonchus contortus, Mangifera indica L.

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“…Mesquita-Sousa et al . [ 18 ] noted that Citrus aurantium var. is effective when used in combination.…”

Section: Resultsmentioning

confidence: 99%

Anthelmintic effects of Podang mango (Mangifera indica) fruit peel waste extract through in vivo application on Indonesian Etawa goat production and health

Prasetyo,

Rokana,

Baihaqi

et al. 2024

Vet World

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The phytotherapy properties of winter savory (Satureja montana L.) essential oil against gastrointestinal nematodes of sheep

Štrbac,

Krnjajić,

Ratajac

et al. 2024

Preprint

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Background: The increasing difficulties in combating anthelmintic resistance in gastrointestinal nematodes (GINs) of sheep worldwide and the residues of chemical drugs in animal products and the environment necessitate the search for alternatives. Previous studies have shown that plant essential oils (EOs) could be valuable anthelmintic agents. The aim of this study was to evaluate the possibility of using winter savory (Satureja montana L.) EO against sheep GINs through performing in vitro and in vivo efficacy tests and preliminary toxicity studies. Methods: The chemical composition of the S. montana EO was determined by gas chromatography-mass spectrometry (GC-MS). For the evaluation of anthelmintic potential in vitro, the Egg Hatch Test (EHT) was performed at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml). Field efficacy was evaluated using the Faecal Egg Count Reduction Test (FECRT) on two farms where the oil was applied intraruminally to sheep at a mean dose of 150 mg/kg. The toxic effect of the oil was evaluated by physical observation of the tested animals and by collecting blood samples for further haematological and biochemical analyses. Coproculture examination of faecal samples from the tested sheep before and after treatment was also performed to identify the GIN genera present. Results: Main compounds of the S. montana oil identified by GC-MS analysis were p-cymene (42.8%), carvacrol (28.1%) and y-terpinene (14.6%). The in vitro EHT showed a dose-dependent (R2=0.94) anthelmintic potential of the tested oil with ovicidal activity varying from 17.0-83.3% and determined IC50 value of 0.59 mg/ml. The field efficacy reached 33% (at group level) and 50% (at individual level) at D14 after treatment. In vivo efficacy was significantly higher in farm 2 (FEC above 65% at group level, p<0.05) where sheep were kept in pens during treatment. No toxic effects were observed either in the physical observation of the test animals or in their liver and kidney function. Conclusion: The moderate efficacy on EHT and FECRT, without adverse effects on the sheep, suggests that S. montana EO is suitable for the control of sheep GINs as part of an integrated parasite management. Further studies should be conducted to further increase efficacy.

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Evaluation of a combination of Citrus aurantium var. Dulcis essential oil and albendazole for the treatment of sheep gastrointestinal nematodes

Cited by 2 publications

References 39 publications

Anthelmintic effects of Podang mango (Mangifera indica) fruit peel waste extract through in vivo application on Indonesian Etawa goat production and health

Anthelmintic effects of Podang mango (Mangifera indica) fruit peel waste extract through in vivo application on Indonesian Etawa goat production and health

The phytotherapy properties of winter savory (Satureja montana L.) essential oil against gastrointestinal nematodes of sheep

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