Evaluation of a commercial assay for the detection of mumps specific IgM antibodies in oral fluid and serum specimens

Warrener, Lenesha; Samuel, Dhanraj

doi:10.1016/j.jcv.2005.10.008

Cited by 24 publications

(17 citation statements)

References 14 publications

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“…Asymptomatic patients can also transmit the virus. Natural infection was once thought to confer lifelong immunity, but we now know that re-infection can occur, although it tends to be milder and more atypical than in primary infection [40,41].…”

Section: Epidemiologymentioning

confidence: 99%

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The increasing incidence of mumps orchitis: a comprehensive review

et al. 2010

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There has been a recent increase in mumps orchitis among pubertal and postpubertal males. These outbreaks can be attributed to a reduction in the uptake of measles‐mumps‐rubella (MMR) vaccine during the early to mid‐1990s in children who have now matured. The mumps virus is commonly associated with extra‐salivary complications. Unvaccinated postpubertal males diagnosed with mumps virus frequently develop complications such as mumps orchitis. Therefore, it is important that urologists are familiar with the diagnosis, treatment and complications of this condition. Here we review the epidemiology, clinical presentation, diagnostic methods, treatment options and complications of mumps orchitis, as a complication of mumps virus, with particular emphasis on testicular atrophy, subfertility and infertility.

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Section: Epidemiologymentioning

confidence: 99%

“…The virus specific IgM antibody can be measured by direct or indirect ELISA techniques 7-10 days after the onset of symptoms [41,51]. The sensitivity of IgM testing is variable, being as low as 24-51% in one study of five ELISA assays [51].…”

Section: Diagnosismentioning

confidence: 99%

The increasing incidence of mumps orchitis: a comprehensive review

et al. 2010

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“…More than 20 min exposure of the surface to the streptavidin-gold did not make a difference in the amount of gold nanoparticles bound to the surface as determined by absorbance measurements alone. Figure 2 shows the appearance of glass slides with deposited silver on slides exposed to different concentrations of colloidal goldstreptavidin conjugate (0, 2.5, 5, 10, and 20%) for different amounts of time (10,15,20,25,30 and 60 min). The visual appearance of the samples indicates different extents of silver deposition.…”

Section: Resultsmentioning

confidence: 99%

“…Samples with immobilized BSA and biot-BSA were exposed to the different dilutions for different amounts of time (5,10,15,20,25,30 and 60 min). The surfaces were thoroughly rinsed with water, dried with N 2 and the plasmon resonance peak characteristic of gold nanoparticles was measured to assess the gold deposition for the different slides using a Shimadzu UV-2401PC spectrophotometer.…”

Section: Surface Immobilization Of Bsa and Biot-bsamentioning

confidence: 99%

“…In conjunction with commonly used detection schemes such as fluorometric [3,7], spectrophotometric, electrochemical [8], and optical [9], among others, immunoassays are becoming more prominent due to their specificity and reliability. Immunoassays are based on the biospecific molecular recognition interaction of antibodies with the target substances (antigens) to be detected and have been widely used to measure low concentrations of antigens in clinical specimens such as blood and other body fluids [1,10] and the detection of trace amounts of drugs, chemicals (e.g., pesticides), bacteria and viruses in biological and environmental samples [11]. Immunoassays can be enzyme-based (such as the enzyme linked immunosorbent assay or ELISA), fluorescent (with a fluorescent molecule covalently linked to a biomolecule), chemiluminescent (luminescence due to chemical reaction), bioluminescent (luminescence due to biological reaction) or metallo-immunoassay (metal-based labels such as metal ions or colloidal metal nanoparticles).…”

Section: Introductionmentioning

confidence: 99%

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Rapid colorimetric detection of proteins and bacteria using silver reduction/precipitation catalyzed by gold nanoparticles

Betala

Appugounder

Chakraborty

et al. 2007

Sens. & Instrumen. Food Qual.

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A colorimetric immunoassay has been developed based on the reduction and precipitation of silver ions catalyzed by colloidal gold. This method was evaluated for a noncompetitive heterogeneous immunoassay to detect ovalbumin as a model protein and Escherichia coli as model bacterium. The influence of relevant experimental variables, including the reaction time of antigen with antibody, the dilution ratio of the colloidal gold-labeled protein, time allowed for silver reduction/precipitation reaction and other parameters were examined and optimized. This colorimetric method of detection is convenient, easy to use and economic. The presence of the target molecules or antigens can be visually inferred based on the color change that occurs in a short time without using major instrumentation. This method is widely amenable for detection of proteins, viruses and bacteria.

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