Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Tran, Na T. D.; Nu, Phuong Anh Ton; Intuyod, Kitti; Dao, Ly T. K.; Pinlaor, Porntip; Nawa, Yukifumi; Choowongkomon, Kiattawee; Geadkaew-Krenc, Amornrat; Kosa, Nanthawat; Grams, Rudi; Pinlaor, Somchai

doi:10.4269/ajtmh.18-0833

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Comparison with other methods that can identify samples from cattle with pre-patent or early infections distinguishes antigen detection, which is undetectable by the usual parasitological tests. In addition, these assays give a more accurate indication of the current state of infections [13]. In a previous study, Attallah et al [10] identified a band at 27 kDa during E/S antigenic examinations in the adult worm of F. gigantica and the sera of humans infected with F. gigantica.…”

Section: Discussionmentioning

confidence: 99%

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

et al. 2021

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Background and Aim: Diagnosis of fascioliasis depends on clinical symptoms and routine laboratory tests. Recently, antibodies and circulating antigens of Fasciola were used for detecting active infections. Therefore, this study aimed to identify Fasciola gigantica antigens in the sera of infected cattle using Western blotting and enzyme-linked immunosorbent assay (ELISA) for an accurate diagnosis of cattle infected with F. gigantica. Materials and Methods: Serum samples were obtained from 108, 23, and 19 cattle infected with Fasciola gigantica, Paramphistomum cervi, and Strongylids, respectively, including 57 non-infected cattle that were used as healthy cattle for the study. Western blotting and ELISA were then used to detect circulating Fasciola antigens at 27 kDa. Results: The target epitope was detected in an F. gigantica adult-worm antigen preparation, excretory/secretory products, and serum from cattle infected with F. gigantica. However, it was absent in sera from P. cervi, Strongylids, and healthy cattle. The purified 27 kDa F. gigantica (FPA-27) antigen was also detected in cattle serum using ELISA with high degrees of sensitivity and specificity (94% and 82%, respectively), and the area under the receiver operating characteristic curve was 0.89 with a highly significant correlation of p<0.0001. Conclusion: The FPA-27 is proposed to be a promising candidate for the serodiagnosis of fascioliasis in cattle.

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Section: Discussionmentioning

confidence: 99%

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

et al. 2021

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“…In the hepatic phase, serological tests like enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) tests are expected to have a high diagnostic yield. 3,8 But due to a lack of easy availability, both of these tests could not be used on our patient.…”

Section: Discussionmentioning

confidence: 99%

“…Owing to a long period of latency and a biphasic mode of presentation, early identification of fascioliasis continues to pose a diagnostic challenge. In the hepatic phase, serological tests like enzyme‐linked immunosorbent assay (ELISA) and indirect hemagglutination (IHA) tests are expected to have a high diagnostic yield 3,8 . But due to a lack of easy availability, both of these tests could not be used on our patient.…”

Section: Discussionmentioning

confidence: 99%

Liver fluke in a young Nepalese girl: A rare diagnostic puzzle

Pandey,

Mishra,

Khadka

et al. 2023

Clinical Case Reports

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Key Clinical messageFascioliasis poses diagnostic challenges to clinicians for its broad spectrum of hepatobiliary symptoms and lower detection rates. Timely and precise identification avoids long‐standing hepatic complications.AbstractFasciola hepatica, a trematode parasite, inhabits snails and sheep. Human hepatic fascioliasis is a neglected tropical disease with no specific tests. In this instance, the significance of clinical awareness, appropriate imaging, and serological investigations is demonstrated. A young Nepalese girl was diagnosed and managed successfully.

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“…Recombinant proteins and synthetic peptides based on cysteine proteases are used in immunodiagnostic assays (Intapan et al 2005;Tantrawatpan et al 2005;Gonzales-Santana et al 2013;Meshgi et al 2018). The cysteine protease-based ELISAs were further simplified by using cystatin-capture (CC)-ELISA to detect anti-cysteine protease antibodies without the need for a purified or recombinant cysteine protease; CC-ELISA using whole worm extract was reported suitable for practical diagnosis of fasciolosis (Ikeda 1998;Tantrawatpan et al 2005;Tran et al 2019). Pen-side diagnostic tests have been evaluated to make diagnosis easier, faster, and more cost-effective.…”

Section: Diagnostic Approaches For Human Fasciolosismentioning

confidence: 99%

“…2005; Tran et al . 2019). Pen-side diagnostic tests have been evaluated to make diagnosis easier, faster, and more cost-effective.…”

Section: Diagnostic Approaches For Human Fasciolosismentioning

confidence: 99%

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Aftab,

Raina,

Maxton

et al. 2024

J. Helminthol.

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Fasciolosis, caused by Fasciola hepatica and F. gigantica, is an impediment to the livestock industry’s expansion and has a massively negative socio-economic impact due to its widespread prevalence in livestock. It is a waterborne zoonosis affecting human populations in the countries where rural economies are associated with livestock rearing. Conventional diagnosis of Fasciola infection is done by detecting parasite eggs in the faeces of infected animals or by immunological methods. Accurate and quick immunodiagnosis of Fasciola infection in animals and humans is based on the detection of antibodies and specific antigens expressed in the prepatent stage of the parasite. Both molecular and serodiagnostic tests developed thus far have enhanced the reliability of Fasciola diagnosis in both man and animals but are not widely available in resource-poor nations. A pen-side diagnostic test based on a lateral flow assay or a DNA test like loop-mediated isothermal amplification (LAMP) would be simple, fast, and cost-effective, enabling clinicians to treat animals in a targeted manner and avoid the development of drug resistance to the limited flukicides. This review focuses on the recent advances made in the diagnosis of this parasite infection in animals and humans.

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Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit and In-House Fasciola gigantica Cysteine Proteinases-Based Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Fascioliasis

Cited by 5 publications

References 38 publications

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

Liver fluke in a young Nepalese girl: A rare diagnostic puzzle

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

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