Evaluation of a commercial lateral flow feed test for rapid detection of beef and sheep content in raw and cooked meats

Rao, Qinchun; Hsieh, Y.-H. Peggy

doi:10.1016/j.meatsci.2006.12.011

Cited by 33 publications

(14 citation statements)

References 11 publications

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“…For meat species and meat adulteration detection, many studies were reported. e methods used included molecular biology-based methods [5,6], enzymatic immunological methods [7], chromatographic methods [8,9], spectroscopy methods [10][11][12], and electronic sensory evaluation [9,13].…”

Section: Introductionmentioning

confidence: 99%

Combination of an E-Nose and an E-Tongue for Adulteration Detection of Minced Mutton Mixed with Pork

Xiao

Wang

et al. 2019

Journal of Food Quality

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An E-panel, comprising an electronic nose (E-nose) and an electronic tongue (E-tongue), was used to distinguish the organoleptic characteristics of minced mutton adulterated with different proportions of pork. Meanwhile, the normalization, stepwise linear discriminant analysis (step-LDA), and principle component analysis were employed to merge the data matrix of E-nose and E-tongue. The discrimination results were evaluated and compared by canonical discriminant analysis (CDA) and Bayesian discriminant analysis (BAD). It was shown that the capability of discrimination of the combined system (classification error 0%∼1.67%) was superior or equable to that obtained with the two instruments separately, and E-tongue system (classification error for E-tongue 0∼2.5%) obtained higher accuracy than E-nose (classification error 0.83%∼10.83% for E-nose). For the combined system, the combination of extracted data of 6 PCs of E-nose and 5 PCs of E-tongue was proved to be the most effective method. In order to predict the pork proportion in adulterated mutton, multiple linear regression (MLR), partial least square analysis (PLS), and backpropagation neural network (BPNN) regression models were used, and the results were compared, aiming at building effective predictive models. Good correlations were found between the signals obtained from E-tongue, E-nose, and fusion data of E-nose and E-tongue and proportions of pork in minced mutton with correlation coefficients higher than 0.90 in the calibration and validation data sets. And BPNN was proved to be the most effective method for the prediction of pork proportions with R2 higher than 0.97 both for the calibration and validation data set. These results indicated that integration of E-nose and E-tongue could be a useful tool for the detection of mutton adulteration.

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Section: Introductionmentioning

confidence: 99%

Combination of an E-Nose and an E-Tongue for Adulteration Detection of Minced Mutton Mixed with Pork

Xiao

Wang

et al. 2019

Journal of Food Quality

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“…Several methods such as electrophoresis, chromatography and DNA-based assays are available for the detection of all animal species present in the meat products (Hsieh et al 1995;Macedo-Silva et al 2000;Rodríguez et al 2004;Kesmen et al 2007;Rao & Hsieh 2007). Evaluation of collagen content is the major method for the tissue differentiation in the meat products using spectroscopic and chromatographic techniques (Colgrave et al 2008).…”

mentioning

confidence: 99%

Evaluation of the morphologic method for the detection of animal and herbal content in minced meat

Sadeghinezhad¹,

Hajimohammadi²,

Izadi³

et al. 2015

Czech J. Food Sci.

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Sadeghinezhad J., Hajimohammadi B., Izadi F., Yarmahmoudi F., Latorre R. (2015): Evaluation of the morphologic method for the detection of animal and herbal content in minced meat. Czech J. Food Sci., 33: 564-569.The quantitative and qualitative accuracy of the routine histological method for the determination of unauthorised animal and herbal content in minced meat was to evaluated. Laboratory adulterated minced beef meat; each containing 5, 10, 15 and 20% of soya and chicken gizzard was prepared. Then each sample was divided into three parts and four paraffin embedded blocks were prepared from each part. The sections were stained using haematoxylin and eosin, toluidine blue and Masson's trichrome. The histological examination revealed the soya and gizzard tissues clearly in all the samples. The histometrical analysis showed that there was no significant difference between the estimated percentages of both additive tissues and the real related percentages. Overall, neither was there any significant difference between the data of the three parts of each sample and the real percentages. The findings of the present research suggest the histological technique as an effective method for qualitative and quantitative evaluations of minced meat.

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“…First, using pure water instead of the extraction buffer for sample preparation may cause aberrant result. The salt-soluble protein cannot be extracted adequately from meat by pure water [27]. The target antigen, Mb, is a salt-soluble protein in meat and therefore we chose PBS containing 0.1% BSA as the extraction buffer for this strip.…”

Section: Discussionmentioning

confidence: 99%

Rapid Immune Colloidal Gold Strip for Cetacean Meat Restraining Illegal Trade and Consumption: Implications for Conservation and Public Health

Chin

Chu

et al. 2013

PLoS ONE

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The consumption of cetacean meat is geographically common and often of undetermined sustainability. Besides, it can expose humans to contaminants and zoonotic pathogens. The illegality of possessing cetacean meat was likely under-reported in some countries due to lack of attention paid by the officials although DNA analysis of market products helped to show such practices. We developed two monoclonal antibodies against synthetic peptides of myoglobin (Mb) for constructing a rapid immune colloidal gold strip. Only cetacean Mb is capable of binding to both antibodies and presents positive signal while the Mb from other animals can bind only 1 of the antibodies and presents negative result. The strip for cetacean meat would be an applicable and cost-effective test for field inspectors and even the general public. It contributes to increase the reporting capacity and coverage of illegal cetacean meat possession, which has implications for global cetacean conservation and public health.

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Evaluation of a commercial lateral flow feed test for rapid detection of beef and sheep content in raw and cooked meats

Cited by 33 publications

References 11 publications

Combination of an E-Nose and an E-Tongue for Adulteration Detection of Minced Mutton Mixed with Pork

Combination of an E-Nose and an E-Tongue for Adulteration Detection of Minced Mutton Mixed with Pork

Evaluation of the morphologic method for the detection of animal and herbal content in minced meat

Rapid Immune Colloidal Gold Strip for Cetacean Meat Restraining Illegal Trade and Consumption: Implications for Conservation and Public Health

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