Evaluation of a commercial multiplex PCR for diagnosis of central nervous system (CNS) nosocomial infections

López-Amor, Lucía; García-Prieto, Emilio; Fernández-Suarez, Jonathan; Escudero, D.; Vázquez, Fernando; Fernández, Javier

doi:10.1016/j.mimet.2020.105865

Cited by 6 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All studies used an acceptable reference standard, collected the specimen for both index and reference testing at the same time, used the same reference standard for all patients, and avoided a case‒control study design. In summary, three studies were labelled ‘unclear’ for patient selection [29, 37, 46–48], two studies were labelled ‘high-risk’ for index tests [36, 46], three studies were labelled ‘unclear’ for index tests [29, 38, 49], three studies were labelled ‘unclear’ for reference standards [29, 50, 51], two studies were labelled ‘high-risk’ for flow and timing [29, 48, 52] and six studies were labelled ‘unclear’ for flow and timing [37, 46, 49, 51, 53–55]. Overall, all of the studies had low concern regarding applicability.…”

Section: Resultsmentioning

confidence: 99%

“…FP, false positive; TP, true positive; Table 1. Continued [29,37,[46][47][48], two studies were labelled 'high-risk' for index tests [36,46], three studies were labelled 'unclear' for index tests [29,38,49], three studies were labelled 'unclear' for reference standards [29,50,51], two studies were labelled 'high-risk' for flow and timing [29,48,52] and six studies were labelled 'unclear' for flow and timing [37,46,49,51,[53][54][55]. Overall, all of the studies had low concern regarding applicability.…”

Section: Quality Assessmentmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

Yang,

Tao

2023

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. The FilmArray blood culture identification panel (BCID) panel is a multiplex PCR assay with high sensitivity and specificity to identify the most common pathogens in bloodstream infections (BSIs). Hypothesis. We hypothesize that the BCID panel has good diagnostic performance for BSIs and can be popularized in clinical application. Aim: To provide summarized evidence for the diagnostic accuracy of the BCID panel for the identification of positive blood cultures. Methodology. We searched the MEDLINE, EMBASE and Cochrane databases through March 2021 and assessed the efficacy of the diagnostic test of the BCID panel. We performed a meta-analysis and calculated the summary sensitivity and specificity of the BCID panel. Systematic review protocols were registered in the International Prospective Register of Systematic Reviews (PROSPERO) (registration number CRD42021239176). Results. A total of 16 full-text articles were eligible for analysis. The overall sensitivities of the BCID panel on Gram-positive bacteria, Gram-negative bacteria and fungi were 97 % (95 % CI, 0.96–0.98), 100 % (95 % CI, 0.98–01.00) and 99 % (95 % CI, 0.87–1.00), respectively. The pooled diagnostic specificities were 99 % (95 % CI, 0.97–1.00), 100 % (95 % CI, 1.00–1.00) and 100 % (95 % CI, 1.00–1.00) for Gram-positive bacteria, Gram-negative bacteria and fungi, respectively. Conclusions. The BCID panel has high rule-in value for the early detection of BSI patients. The BCID panel can still provide valuable information for ruling out bacteremia or fungemia in populations with low pretest probability.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Quality Assessmentmentioning

confidence: 99%

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

Yang,

Tao

2023

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…73 A recent study analyzed CSF samples of patients with CNS infections using the BCID panel and reported promising results. 74 Ninety CSF samples were analyzed with the BCID panel achieving a sensitivity of 77.4% and specificity of 100%, while the use of lower cut-offs, improved the sensitivity to 83.9% without any significant impact on a specificity that remained high (98.3%). 74 The turnaround time of both ME and BCID panels is much faster than conventional cultures, approximately 1 hour.…”

Section: Dovepressmentioning

confidence: 99%

“…74 Ninety CSF samples were analyzed with the BCID panel achieving a sensitivity of 77.4% and specificity of 100%, while the use of lower cut-offs, improved the sensitivity to 83.9% without any significant impact on a specificity that remained high (98.3%). 74 The turnaround time of both ME and BCID panels is much faster than conventional cultures, approximately 1 hour. 72,73 An alternative technique for the molecular identification of the pathogen is to process an aliquot of a positive CSF culture into a micro/nanofluidic chip platform that contains many genetic targets, species-specific or resistance determinants.…”

Section: Dovepressmentioning

confidence: 99%

Current Perspectives on the Diagnosis and Management of Healthcare-Associated Ventriculitis and Meningitis

Karvouniaris¹,

Brotis²,

Tsiakos³

et al. 2022

IDR

View full text Add to dashboard Cite

Ventriculitis or post-neurosurgical meningitis or healthcare-associated ventriculitis and meningitis (VM) is a severe infection that complicates central nervous system operations or is related to the use of neurosurgical devices or drainage catheters. It can further deteriorate patients who have already presented significant neurologic injury and is associated with high morbidity, mortality, and poor functional outcome. VM can be difficult to distinguish from aseptic meningitis, inflammation that follows hemorrhagic strokes and neurosurgical operations. The associated microorganisms can be either skin flora or nosocomial pathogens, most commonly, Gram-negative bacteria. Classical microbiology can fail to isolate the culprit pathogen. Novel cerebrospinal fluid (CSF) biomarkers and molecular microbiology can fill the diagnostic gap and expedite pathogen identification and treatment. The pathogens may demonstrate significant resistant patterns and their antibiotic treatment can be difficult, as many important drug classes, including the beta-lactams and the glycopeptides, hardly penetrate to the CSF, and do not achieve therapeutic levels at the site of the infection. Treatment modifications, such as higher daily dose and prolonged or continuous administration, might increase antibiotic levels in the site of infection and facilitate pathogens clearance. However, in the case of therapeutic failure or infection due to difficultto-treat bacteria, the direct antibiotic instillation into the CSF, in addition to the intravenous antibiotic delivery, may help in the resolution of infection. However, intraventricular antibiotic therapy may result in aseptic meningitis and seizures, concerning the administration of aminoglycosides, polymyxins, and vancomycin. Meanwhile, bacteria form biofilms on the catheter or the device that should routinely be removed. Novel neurosurgical treatment modalities comprise endoscopic evacuation of debris and irrigation of the ventricles. VM prevention includes perioperative antibiotics, antimicrobial impregnated catheters, and the implementation of standardized protocols, regarding catheter insertion and manipulation.

show abstract

Utilidad del PCR múltiplex de hemocultivo para el diagnóstico microbiológico de infecciones nosocomiales del sistema nervioso central en pacientes críticos

Mauro,

Verga,

Galiana

et al. 2024

Acta Colombiana de Cuidado Intensivo

View full text Add to dashboard Cite

Evaluation of a commercial multiplex PCR for diagnosis of central nervous system (CNS) nosocomial infections

Cited by 6 publications

References 20 publications

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

Diagnostic efficiency of the FilmArray blood culture identification (BCID) panel: a systematic review and meta-analysis

Current Perspectives on the Diagnosis and Management of Healthcare-Associated Ventriculitis and Meningitis

Utilidad del PCR múltiplex de hemocultivo para el diagnóstico microbiológico de infecciones nosocomiales del sistema nervioso central en pacientes críticos

Contact Info

Product

Resources

About