Evaluation of a DNA-Hybridization Method for Detection of African and Asian Strains of Neisseria gonorrhoeae in Men with Urethritis

Perine, Peter L.; Totten, P A; Holmes, K. K.; Sng, E H; Ratnam, A. V.; Widy-Wersky, R.; Nsanze, H; Habte-Gabr, Eyassu; Westbrook, W G

doi:10.1093/infdis/152.1.59

Cited by 35 publications

(10 citation statements)

References 16 publications

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“…The aminopeptidase F 16 meningococci showed a typical aminopeptidase profile that is, glutamyl aminopeptidase activity but not hydroxyproline aminopeptidare activity (table 1). The remaining three strains showed the converse, which is typical of N gonorrhoeae or N lactamica.…”

Section: Resultsmentioning

confidence: 99%

Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

Ison¹,

Bellinger²,

Walker³

1986

Journal of Clinical Pathology

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SUMMARY DNA probe hybridisation was used to examine the relation between the cryptic plasmid from Neisseria gonorrhoeae and plasmids carried by pharyngeal isolates of Neisseria meningitidis and Neisseria lactamica. The complete gonococcal cryptic plasmid and HinfI derived digestion fragments subcloned into Escherichia coli were used to probe Southern blots of plasmid extracts. Homology was found to a plasmid of approximate molecular weight 4-5 kilobase pairs (Kb) but not to plasmids of < 3-2 Kb or 6 5 Kb. Eleven of 16 strains of N meningitidis and two of six strains of N lactamica carried plasmids that showed strong hybridisation with the 4 2 Kb gonococcal plasmid. Hybridisation of plasmids from non-gonococcal species of neisseria with the gonococcal cryptic plasmid indicates that caution should be taken when using the cryptic plasmid as a diagnostic probe for gonorrhoea.The genus Neisseria contains two pathogenic species, Neisseria meningitidis and Neisseria gonorrhoeae. N meningitidis colonises the upper respiratory tract and invades to produce systemic disease, whereas N gonorrhoeae infects the columnar epithelium of the genital tract and rectum and only rarely causes systemic disease. Other species of neisseria have occasionally been associated with infection but are generally regarded as commensals.' Within the genus there is strong DNA homology between the species,2 which is highest between N meningitidis and N gonorrhoeae (> 80%). Despite their close genetic association pathogenic neisseria can be distinguished from commensal neisseria by their ability to produce IgAl protease,3 adhere to columnar epithelium,4 and produce an outer membrane antigen designated H8.s N meningitidis and N gonorrhoeae differ from each other in their ability to utilise carbohydrates, requirement for cysteine and cystine, and their aminopeptidase profiles.6 In most clinical laboratories only the ability of N meningitidis to produce acid from maltose in addition to glucose is routinely used for differentiation.A major distinguishing factor is the carriage of plasmids by strains of N gonorrhoeae.7 Several plas-

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Section: Resultsmentioning

confidence: 99%

Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

Ison¹,

Bellinger²,

Walker³

1986

Journal of Clinical Pathology

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“…The probe assay was 91% sensitive and 96% specific when compared with the chromogenic cephalosporin test performed on isolated colonies of N. gonorrhoeae (26). More studies of this type (i.e., parallel identification and susceptibility testing with probes) would be of value for determining the feasibility of probes as rapid diagnostic aids for physicians offices, outpatient clinics, and Third World countries.…”

Section: Aminoglycoside Resistance Genesmentioning

confidence: 99%

Studies of antimicrobial resistance genes using DNA probes

Tenover¹

1986

Antimicrob Agents Chemother

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“…Five "false negatives" turned out to harbor plasmid-lacking Neisseria. Perine et al (94) detected Neisseria urethritis in urethral exudates of men with clinical symptoms of gonococcal urethritis using a similar assay and reported that 180 of 216 samples were positive by hybridization and culture; 27 were negative by both tests. Hyyp'a et al …”

Section: Sexually Transmitted Diseases and Genital Infectionsmentioning

confidence: 99%

The state of the art of clinical gene diagnosis

Gillespie

1987

Clinical Laboratory Analysis

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Gene diagnosis is defined and evaluated in light of existing methods of clinical diagnosis. Gene diagnosis for diseases caused by somatic or inherited mutations is more powerful than existing clinical methods, but the state of this art is too cumbersome and expensive for routine diagnosis. Gene diagnosis for infectious disease is more specific than existing detection methods, but usually is not yet as sensitive as culture or as advanced. The format for both forms of gene diagnosis will evolve to liquid hybridization followed by a “dot” format for evaluating results.

show abstract

Evaluation of a DNA-Hybridization Method for Detection of African and Asian Strains of Neisseria gonorrhoeae in Men with Urethritis

Cited by 35 publications

References 16 publications

Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

Studies of antimicrobial resistance genes using DNA probes

The state of the art of clinical gene diagnosis

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