Evaluation of a hospital-based injury surveillance system for monitoring road traffic deaths in Phuket, Thailand

Nittayasoot, Natthaprang; Peterson, Alexis B.; Thammawijaya, Panithee; Parker, Erin; Sathawornwiwat, Apichit; Boonthanapat, Naris; Chantian, Thanawadee; Voradetwitaya, Lujisak; Jiraphongsa, Chuleeporn; Sagarasaeranee, Onpirun; Sansilapin, Chalo; Rattanathamsakul, Thanit; Ketgudee, Lalida; Tantiworrawit, Patcharin

doi:10.1080/15389588.2019.1581924

Cited by 7 publications

(6 citation statements)

References 5 publications

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“…Traumatic brain injury (TBI) is an underrecognized threat to public health. Approximately 2.8 million Americans sustain a TBI each year (1). Injurious inflammatory, hypoxic, ischemic, and excitotoxic mechanisms follow the initial insult increasing the secondary impact of the injury.…”

Section: Introductionmentioning

confidence: 99%

Fecal Microbiota Transfer Attenuates Gut Dysbiosis and Functional Deficits After Traumatic Brain Injury

Davis¹,

Chen

Islam³

et al. 2022

Shock

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Background: Traumatic brain injury (TBI) is an underrecognized public health threat. Survivors of TBI often suffer long-term neurocognitive deficits leading to the progressive onset of neurodegenerative disease. Recent data suggests that the gut-brain axis is complicit in this process. However, no study has specifically addressed whether fecal microbiota transfer (FMT) attenuates neurologic deficits after TBI. Hypothesis: We hypothesized that fecal microbiota transfer would attenuate neurocognitive, anatomic, and pathologic deficits after TBI. Methods: C57Bl/6 mice were subjected to severe TBI (n ¼ 20) or sham-injury (n ¼ 20) via an open-head controlled cortical impact. Post-injury, this cohort of mice underwent weekly oral gavage with a slurry of healthy mouse stool or vehicle alone beginning 1 h post-TBI followed by behavioral testing and neuropathologic analysis. 16S ribosomal RNA sequencing of fecal samples was performed to characterize gut microbial community structure pre-and post-injury. Zero maze and open field testing were used to evaluate post-traumatic anxiety, exploratory behavior, and generalized activity. 3D, contrast enhanced, magnetic resonance imaging was used to determine differences in cortical volume loss and white matter connectivity. Prior to euthanasia, brains were harvested for neuropathologic analysis. Results: Fecal microbiome analysis revealed a large variance between TBI, and sham animals treated with vehicle, while FMT treated TBI mice had restoration of gut dysbiosis back to levels of control mice. Neurocognitive testing demonstrated a rescue of normal anxiety-like and exploratory behavior in TBI mice treated with FMT. FMT treated TBI mice spent a greater percentage of time (22%, P ¼ 0.0001) in the center regions of the Open Field as compared to vehicle treated TBI mice (13%). Vehicle-treated TBI animals also spent less time (19%) in the open areas of zero maze than FMT treated TBI mice (30%, P ¼ 0.0001). Comparing in TBI mice treated with FMT, MRI demonstrated a marked attenuation in ventriculomegaly (P < 0.002) and a significant change in fractional anisotropy (i.e., loss of white matter connectivity) (P < 0.0001). Histologic analysis of brain sections revealed a FMT-injury dependent interaction in the microglia/macrophage-specific ionized calcium-binding protein, Iba1 (P ¼ 0.002). Conclusion: These data suggest that restoring a pre-injury gut microbial community structure may be a promising therapeutic intervention after TBI.

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Section: Introductionmentioning

confidence: 99%

Fecal Microbiota Transfer Attenuates Gut Dysbiosis and Functional Deficits After Traumatic Brain Injury

Davis¹,

Chen

Islam³

et al. 2022

Shock

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“…Its assessment was not considered relevant given the nature of the phenomenon being monitored. An evaluation of the injury surveillance system in monitoring RC deaths in Pakistan estimated its PPV at 99% (Nittayasoot et al, 2019). RTI victims are admitted to health facilities after the crash has occurred.…”

Section: Limitationsmentioning

confidence: 99%

Performance of the routine health information system for epidemiological surveillance of road traffic injuries in Benin

̈houen¹,

̈-Ahanhanzo²,

Sopoh³

et al. 2020

J. Public Health Epidemiol.

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Road crashes are the leading cause of death among people aged 15 and above. The routine health information system is used to monitor common hospital illnesses, including road traffic injuries. The objective of this study was to evaluate its performance in the surveillance of road traffic injuries in Benin in 2019. This was an evaluative study, based on updated guidelines from the Centre for Disease Prevention and Control to evaluate a public health surveillance system. Performance was assessed on the basis of utility and quality criteria (simplicity, flexibility, acceptability, completeness, exhaustiveness, stability, responsiveness, representativeness and reactivity). The data collection techniques used was document review and interview. The system was considered useful and produced indicators on road traffic injuries each year within an average of five months. Its strengths were its representativeness (100%), stability (100%) and simplicity (83%). Acceptability, exhaustiveness and completeness were the main areas for improvement in the system with scores of 51.4, 38.07 and 16.27%, respectively. The routine health information system presents indicators of insufficient acceptability and low integration with other existing collection systems for road traffic injuries. Improving performance for better planning in road safety requires a more holistic approach in the implementation and functionality of the system.

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“…Traumatic brain injury (TBI) is an underrecognized public health threat, with more than 10 million people afflicted globally annually (1,2). Victims of TBI have a high rate of neurocognitive and neuropsychiatric morbidity due to the secondary ischemic, hypoxic, excitotoxic, and inflammatory processes that are hallmarks of TBI.…”

Section: Introductionmentioning

confidence: 99%

Postinjury Fecal Microbiome Transplant Decreases Lesion Size and Neuroinflammation in Traumatic Brain Injury

Davis

Chen

Islam

et al. 2022

Shock

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Background: Traumatic brain injury (TBI) is an underrecognized public health threat. The constitutive activation of microglia after TBI has been linked to long-term neurocognitive deficits and the progression of neurodegenerative disease. Evolving evidence indicates a critical role for the gut-brain axis in this process. Specifically, TBI has been shown to induce the depletion of commensal gut bacteria. The resulting gut dysbiosis is associated with neuroinflammation and disease. Hypothesis: We hypothesized that fecal microbiota transplantation would attenuate microglial activation and improve neuropathology after TBI. Methods: C57Bl/6 mice were subjected to severe TBI (n = 10) or sham injury (n = 10) via an open-head controlled cortical impact. The mice underwent fecal microbiota transplantation (FMT) or vehicle alone via oral gavage once weekly for 4 weeks after injury. At 59 days after TBI, mice underwent three-dimensional, contrast-enhanced magnetic resonance imaging. Following imaging, mice were killed, brains harvested at 60 DPI, and CD45+ cells isolated via florescence-activated cell sorting. cDNA libraries were prepared using the 10x Genomics Chromium Single Cell 3′ Reagent kit followed by sequencing on a HiSeq4000 instrument, and computational analysis was performed. Results: Fecal microbiota transplantation resulted in a >marked reduction of ventriculomegaly (P < 0.002) and preservation of white matter connectivity at 59 days after TBI (P < 0.0001). In addition, microglia from FMT-treated mice significantly reduced inflammatory gene expression and enriched pathways involving the heat-shock response compared with mice treated with vehicle alone. Conclusions: We hypothesized that restoring gut microbial community structure via FMT would attenuate microglial activation and reduce neuropathology after TBI. Our data demonstrated significant preservation of cortical volume and white matter connectivity after an injury compared with mice treated with vehicle alone. This preservation of neuroanatomy after TBI was associated with a marked reduction in inflammatory gene expression within the microglia of FMT-treated mice. Microglia from FMT-treated mice enriched pathways in the heat-shock response, which is known to play a neuroprotective role in TBI and other neurodegenerative disease processes.

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Evaluation of a hospital-based injury surveillance system for monitoring road traffic deaths in Phuket, Thailand

Cited by 7 publications

References 5 publications

Fecal Microbiota Transfer Attenuates Gut Dysbiosis and Functional Deficits After Traumatic Brain Injury

Fecal Microbiota Transfer Attenuates Gut Dysbiosis and Functional Deficits After Traumatic Brain Injury

Performance of the routine health information system for epidemiological surveillance of road traffic injuries in Benin

Postinjury Fecal Microbiome Transplant Decreases Lesion Size and Neuroinflammation in Traumatic Brain Injury

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