Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits

Shao, Xin; Hutmacher, Dietmar W.; Ho, Saey Tuan Barnabas; Goh, James Cho Hong; Lee, Eng Hin

doi:10.1016/j.biomaterials.2005.07.040

Cited by 187 publications

(141 citation statements)

References 34 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, MSCs and chondrocytes cultured on such scaffolds showed different tissue morphologies over time [48] . Similar experiments using fibrin glue to mimic the cartilage tissue have also been reported [50,51] . 3D printing using selective laser sintering is also a facile technique to create gradient porosity [52,53] .…”

Section: Three Dimensional (3d) Printing For Osteochondral Defect Heasupporting

confidence: 68%

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Datta

Dhawan

et al. 2024

IJB

View full text Add to dashboard Cite

Abstract:Osteochondral tissue regeneration has remained a critical challenge in orthopaedic surgery, especially due to complications of arthritic degeneration arising out of mechanical dislocations of joints. The common gold standard of autografting has several limitations in presenting tissue engineering strategies to solve the unmet clinical need. However, due to the complexity of joint anatomy, and tissue heterogeneity at the interface, the conventional tissue engineering strategies have certain limitations. The advent of bioprinting has now provided new opportunities for osteochondral tissue engineering. Bioprinting can uniquely mimic the heterogeneous cellular composition and anisotropic extra-cellular matrix (ECM) organization, while allowing for targeted gene delivery to achieve heterotypic differentiation. In this perspective, we discuss the current advances made towards bioprinting of composite osteochondral tissues and present an account of challenges-in terms of tissue integration, long-term survival, and mechanical strength at the time of implantation-required to be addressed for effective clinical translation of bioprinted tissues. Finally, we highlight some of the future trends related to osteochondral bioprinting with the hope of in-clinical translation. Keywords: bioprinting; osteochondral injuries; zonal anisotropy; bioink; tissue engineering

show abstract

Section: Three Dimensional (3d) Printing For Osteochondral Defect Heasupporting

confidence: 68%

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Datta

Dhawan

et al. 2024

IJB

View full text Add to dashboard Cite

show abstract

“…Bone marrow stromal cells (BMSCs) were isolated from five New Zealand White rabbits (average weight, 2.5 kg) according to procedures described previously, 29 and cultured in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C in an incubator with 5% CO 2 . Three kinds of ligaments were seeded with BMSCs at a density of 7.8×10 4 cell/cm 2 .…”

Section: Characterization Of Three Different Ligament Samplesmentioning

confidence: 99%

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Jiang¹,

Wan

Yang

et al. 2014

IJN

View full text Add to dashboard Cite

Background Application of artificial ligament in anterior cruciate ligament reconstruction is one of the research focuses of sports medicine but the biological tendon–bone healing still remains a problem. The preliminary study of hydroxyapatite (HAP) coating on the polyethylene terephthalate (PET) surface could effectively induce the osteoblast differentiation, but the tendon–bone healing was still not stable. As a green synthesis process, the biomimetic mineralization can simulate the natural bone growth in vitro and in vivo. Methods HAP crystals were grown under the guide of silk fibroin (SF) PET surface by biomimetic route. Several techniques including scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy were utilized for proving the introduction of both SF and HAP. The viability and osseointegration of bone marrow stromal cells on the surface of three kinds of ligament, including PET group (non-coating group), PET+SF group (SF-coating group), and PET+SF+HAP group (combined HAP- and SF-coating group), were analyzed by CCK-8 assays and alkaline phosphatase (ALP) detection. Seventy-two mature male New Zealand rabbits were randomly divided into three groups. Among them, 36 rabbits were sacrificed for mechanical testing, and histological examination for the others. Results The SF and SF+HAP were successfully coated on the surface of PET fiber. The CCK-8 assay showed that the cell proliferation on PET+SF+HAP group was better than the other two groups from 24 to 120 hours. After 14 days of culture, the cells in the PET+SF+HAP group delivered higher levels of ALP than the other two groups. After 3 days of culture, the expression level of integrin β1 in the PET+SF+HAP group and PET+SF group were higher than in the PET group. The mean load to failure and the stiffness value of the PET+SF+HAP group were both higher than the other two groups. Hematoxylin and eosin staining showed that new bone tissue formation was only found in the PET+SF+HAP group 8 weeks postoperatively. Masson staining showed that in the PET+SF+HAP group 8 weeks postoperatively, the PET fibers were almost completely encircled by collagen. Histomorphometric analysis showed that the width of the graft–bone interface in the PET+SF+HAP group was narrower than that in the other two groups 4 and 8 weeks postoperatively. The mRNA level of BMP-7 in the PET+SF+HAP groups was significantly higher than those in the other two groups 4 and 8 weeks postoperatively. Conclusion The study showed that the combined SF and HAP coating by biomimetic route on the surface of PET artificial ligament could induce graft osseointegration in the bone tunnel, providing theoretical and experimental foundation for manufacturing novel artificial ligaments meeting the clinical needs.

show abstract

“…As previously described, imaging was performed with 68 mm scan width, an Al-1 mm filter, 35 µm pixel size, using an average of 4 sets of scan data per sample (Shao et al 2006). All samples were scanned through a 180° rotation angle with a rotation step of 0.8° at 35 μm resolution.…”

Section: Micro Computed Tomography (µCt) Scanningmentioning

confidence: 99%

Sustained release and osteogenic potential of heparan sulfate-doped fibrin glue scaffolds within a rat cranial model

et al. 2007

Self Cite

View full text Add to dashboard Cite

This paper explores the potential therapeutic role of the naturally occurring sugar heparan sulfate for the augmentation of bone repair. Scaffolds comprising fibrin glue loaded with 5 µg of embryonically-derived heparan sulfate were assessed, firstly as a releasereservoir, and secondly as a scaffold to stimulate bone regeneration in a critical size rat cranial defect. We show heparan sulfate-loaded scaffolds have a uniform distribution of heparan sulfate, which was readily released with a typical burst phase, quickly followed by a prolonged delivery lasting several days. Importantly, the released heparan sulfate contributed to improved wound healing over a 3 month period as determined by µCT scanning, histology, histomorphometry and PCR for osteogenic markers. In all cases, only minimal healing was observed after 1 and 3 months in the absence of heparan sulfate. In contrast, marked healing was observed by 3 months following heparan sulfate treatment, with nearly full closure of the defect site. PCR analysis showed significant increases in the gene expression of the osteogenic markers Runx2, alkaline phosphatase and osteopontin in the heparin sulfate group compared with controls. These results further emphasize the important role heparan sulfate plays in augmenting wound healing, and its successful delivery in a hydrogel provides a novel alternative to autologous bone graft and growth factor-based therapies.

show abstract

Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits

Cited by 187 publications

References 34 publications

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Sustained release and osteogenic potential of heparan sulfate-doped fibrin glue scaffolds within a rat cranial model

Contact Info

Product

Resources

About

Evaluation of a hybrid scaffold/cell construct in repair of high-load-bearing osteochondral defects in rabbits

Cited by 187 publications

References 34 publications

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Bioprinting of osteochondral tissues: A perspective on current gaps and future trends

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of&nbsp;silk&nbsp;fibroin and depositing of hydroxyapatite

Sustained release and osteogenic potential of heparan sulfate-doped fibrin glue scaffolds within a rat cranial model

Contact Info

Product

Resources

About

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite