Evaluation of a Laser-Based Three-Part Leukocyte Differential Analyzer In Detection of Clinical Abnormalities

Tisdall, Philip A.

doi:10.1093/labmed/16.4.228

Cited by 6 publications

(4 citation statements)

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“…All these findings suggest that caution should be exercised in interpreting three-part dif results in samples with cell morphology deviating from normal. Findings similar to those in this study have been reported earlier for the correlation of results of manual differential counts and three-part difs assayed with other cytometers based on aperture-impedance (20 -22), as well as those based on light-scattering methods (23). The correlation coefficients varied between 0.91 and 0.98 for lymphocytes (typical and variant lymphocytes), between 0.94 and 0.99 for granulocytes (segmented neutrophils and bands), and between 0.56 and 0.65 for mononuclear cells.…”

Section: Discussionsupporting

confidence: 91%

Performance of a Three-Part Dif Impedance Cytometer (Sysmex E-4000) in Comparison with a Cytochemical Cytometer (Technicon H 6000)

Smeets

Wersch²

1988

Clinical Chemistry and Laboratory Medicine

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Particle-size analysis of leukocytes with cytometers based on the impedance method makes it possible to distinguish three types of white cells in blood (small cells, medium-sized cells and large cells). In order to test the ability of such a three-part dif cytometer to screen for abnormal cells of the leukocyte series, we assayed the differentiation of white cells in 786 blood samples with the Sysmex E-4000 and compared the results with those of the Technicon H6000, a cytochemical cytometer. The small cell and large cell fractions of the Sysmex E-4000 correlated very well with the percentages of lymphocytes and neutrophils measured with the Technicon instrument and showed good sensitivity and specificity in discriminating samples with normal and abnormal results. The percentage of the medium-sized cells correlated significantly with the contents of eosinophilic, monocytic and basophilic cells as assayed with the Technicon R6000; but for a satisfactory determination of each of these cell lines (sensitivity of more than 90% and ca. 70% specificity) it was necessary for them to represent more than 10% of the medium-sized cell population. The red cell parameters of both cytometers were compared in 695 samples. All parameters, which were not based on measurement of the red cell volume, exhibited an excellent agreement for both instruments. The mean haematocrit and the average mean corpuscular volume (MCV) were significantly higher and the average mean corpuscular haemoglobin concentration (MCHC) and absolute distribution width (ADW) were significantly lower in the Sysmex E-4000. The mean difference of MCV between both cytometers increased for microcytic and macrocytic cells. There was a great interindividual variation of Δ MCV. No relation between MCHC and mean corpuscular haemoglobin (MCH) was found with the Sysmex E-4000 for normocytic and macrocytic cells, although a significant relation existed for small red cells as a result of overestimation of the MCV in cells with low MCH. In the Technicon H6000 the MCHC correlated significantly with the MCH in normocytic and macrocytic cells, due to underestimation of MCV in these cells. With the Sysmex E-4000 a greater interindividual variation of MCV, and of MCHC in relation to MCH was measured than with the Technicon H6000. Possible explanations of these findings are discussed. ') Abbreviations used: SCR. small cell ratio LCR large cell ratio MCR middle cell ratio MCV mean corpuscular volume = haematocrit/erythrocyte count MCH mean corpuscular haemoglobin = Hb concentration/erythrocyte count MCHC mean corpuscular haemoglobin content = Hb concentration/haematocrit ADW absolute distribution width of the red blood cell volume histogram RDW relative distribution width of the red blood cell volume histogram LUC large unstained cell in Technicon H6000 r Pearson correlation coefficient

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Section: Discussionsupporting

confidence: 91%

Performance of a Three-Part Dif Impedance Cytometer (Sysmex E-4000) in Comparison with a Cytochemical Cytometer (Technicon H 6000)

Smeets

Wersch²

1988

Clinical Chemistry and Laboratory Medicine

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“…Under linear conditions, an r of 1 or −1 reflects total consistency between the results of both methods. An r of >0.95 can be rated as very good and an r of >0.8 as acceptable (Tisdall 1985;Bollinger et al 1987;Tvedten and Wilkins 1988;Winkler et al 1995). In addition to the determination of r, the intercept and the slope have to been considered (Tvedten and Korcal 1996).…”

Section: Linearity Of the Measurement Rangementioning

confidence: 99%

Evaluation of the LaserCyte: an in-house hematology analyzer for dogs and cats

et al. 2006

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In the present study, the LaserCyte instrument, a fully automated flow cytometer for use in veterinary practice, was evaluated for dogs and cats. Precision (coefficient of variation, CV) for red blood cell (RBC) parameters was 3.9%, for reticulocytes between 14.9 and 102%, for white blood cells (WBC) between 3 and 9.5%, for neutrophils between 3.9 and 6.5%, for lymphocytes between 7 and 17.9%, for monocytes between 4.9 and 13.1%, for eosinophils between 10.4 and 32.1%, for basophils between 7.8 and 32%, for platelets between 3.1 and 13.2%, and for platelet indices between 0 and 28.2%. The range of linearity extended the reference ranges. The agreement with reference methods (coefficient of correlation, r) were 0.96 (RBC), 0.94 (hematocrit), 0.96 (hemoglobin), 0.95 (mean corpuscular volume), 0.94 (WBC), 0.93 (neutrophils), 0.77 (lymphocytes), 0.77 (monocytes), 0.29 (eosinophils), 0.03 (basophils), 0.13 (reticulocytes), and 0.86 (platelets). The LaserCyte allowed the correct assessment of RBC and WBC parameters with respect to clinical relevance in the majority of samples. Lymphocytopenia was detected in only 51 out of 89 cases and monocytopenia in one out of 11 cases. The reticulocyte counts were correctly estimated in 85 out of 149 cases. It was concluded that the LaserCyte allowed reliable determination of the RBC parameters, WBCs, neutrophils in both species and platelets in dogs. Based on its capability to reliably determine feline platelets and of the parameters mentioned above, this instrument is considered a useful in-house analyzer for the veterinary practice. Qualitative microscopic assessment of blood smears is still necessary for detecting abnormal cell morphologies, certain cell precursors and blood parasites.

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“…Presently, several measuring tively). The resulting Information yields an effective principles are routinely used in the haematological screening for pathological blood samples, providing lab: The electrical resistance inethod (5), optical meth-important diagnostic Information and reducing the ods (6,7) and cytochemical methods (8,9,10). These number of manual differentiations in the haematocell counting techniques are being continuously im-logical laboratory.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Automated Haematology Analyser Sysmex M-2000

Pohland

1989

Clinical Chemistry and Laboratory Medicine

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Summary:The automated Sysmex M-2000 was evaluated according to the ICSH (International Committee for Standardization in Haematology) protocol. After dilution of packed eells with cell-free plasma, blood cell counts were linear. The overall precision of the measured parameters was good; the CV's ranged between 0.64% and 2.06%. The carry-over was neglible; platelets showed the biggest carry-over with 0.25% in the Whole Blood Mode, while red blood cells (RBC) showed a carry-over of 0.55% in the Prediluted Mode. Pathological samples showed no interference with the blood count. Leukocyte counts less than 1000 χ 10 9 /1 did not effect the measurement of haemoglobin. During the period of evaluation, no Instrument malfunctions occurred.Because of its precision and reliability, the Sysmex M-2000 is well suited for routine work and stat analysis in medium-sized laboratories. Introduction.

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Evaluation of a Laser-Based Three-Part Leukocyte Differential Analyzer In Detection of Clinical Abnormalities

Cited by 6 publications

References 0 publications

Performance of a Three-Part Dif Impedance Cytometer (Sysmex E-4000) in Comparison with a Cytochemical Cytometer (Technicon H 6000)

Performance of a Three-Part Dif Impedance Cytometer (Sysmex E-4000) in Comparison with a Cytochemical Cytometer (Technicon H 6000)

Evaluation of the LaserCyte: an in-house hematology analyzer for dogs and cats

Evaluation of the Automated Haematology Analyser Sysmex M-2000

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